Mycoplasma agalactiae in semen and milk of goat from Pernambuco state, Brazil
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Pesquisa Veterinária Brasileira (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2013001100004 |
Resumo: | In goat and sheep flocks, mycoplasmosis is a disease that may cause severe economical losses associated with polyarthritis, mastitis, agalactia, conjunctivitis, pneumonia and reproductive failure. The latter may involve repeat breeding, granular vulvovaginitis, infertility and abortions. The aim of the present study was to assess the occurrence of Mycoplasma agalactiae (Ma) in semen and milk samples from naturally infected goat in the semiarid region from Pernambuco State, Northeast from Brazil. Thirty-nine semen samples and 81 milk samples were submitted to DNA extraction using a commercially available kit and following the manufacturer's instructions. The polymerase chain reaction (PCR) was then performed in accordance with protocols described in the literature. The results of the present study revealed the presence of Ma in the DNA of 17.9% (7/39) of the semen samples and 3.7% (3/81) of the milk samples. The results obtained in the present study confirm the elimination of the DNA of Ma in the semen and milk samples. The presence of this agent in goat flocks is considered very risky in terms of reproductive disorders and contagious agalactia outbreaks in the Northeast region of Brazil. |
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Mycoplasma agalactiae in semen and milk of goat from Pernambuco state, BrazilMycoplasma agalactiaesemenmilkgoatreproductive disorderIn goat and sheep flocks, mycoplasmosis is a disease that may cause severe economical losses associated with polyarthritis, mastitis, agalactia, conjunctivitis, pneumonia and reproductive failure. The latter may involve repeat breeding, granular vulvovaginitis, infertility and abortions. The aim of the present study was to assess the occurrence of Mycoplasma agalactiae (Ma) in semen and milk samples from naturally infected goat in the semiarid region from Pernambuco State, Northeast from Brazil. Thirty-nine semen samples and 81 milk samples were submitted to DNA extraction using a commercially available kit and following the manufacturer's instructions. The polymerase chain reaction (PCR) was then performed in accordance with protocols described in the literature. The results of the present study revealed the presence of Ma in the DNA of 17.9% (7/39) of the semen samples and 3.7% (3/81) of the milk samples. The results obtained in the present study confirm the elimination of the DNA of Ma in the semen and milk samples. The presence of this agent in goat flocks is considered very risky in terms of reproductive disorders and contagious agalactia outbreaks in the Northeast region of Brazil.Colégio Brasileiro de Patologia Animal - CBPA2013-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2013001100004Pesquisa Veterinária Brasileira v.33 n.11 2013reponame:Pesquisa Veterinária Brasileira (Online)instname:Colégio Brasileiro de Patologia Animal (CBPA)instacron:EMBRAPA10.1590/S0100-736X2013001100004info:eu-repo/semantics/openAccessAlves,Bruno H.L.S.Silva,José G.Mota,André R.Campos,Ana C.Júnior,José W.P.Santos,Sandra B.Mota,Rinaldo A.eng2014-01-21T00:00:00Zoai:scielo:S0100-736X2013001100004Revistahttp://www.pvb.com.br/https://old.scielo.br/oai/scielo-oai.phpcolegio@cbpa.org.br||pvb@pvb.com.br0100-736X1678-5150opendoar:2014-01-21T00:00Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA)false |
dc.title.none.fl_str_mv |
Mycoplasma agalactiae in semen and milk of goat from Pernambuco state, Brazil |
title |
Mycoplasma agalactiae in semen and milk of goat from Pernambuco state, Brazil |
spellingShingle |
Mycoplasma agalactiae in semen and milk of goat from Pernambuco state, Brazil Alves,Bruno H.L.S. Mycoplasma agalactiae semen milk goat reproductive disorder |
title_short |
Mycoplasma agalactiae in semen and milk of goat from Pernambuco state, Brazil |
title_full |
Mycoplasma agalactiae in semen and milk of goat from Pernambuco state, Brazil |
title_fullStr |
Mycoplasma agalactiae in semen and milk of goat from Pernambuco state, Brazil |
title_full_unstemmed |
Mycoplasma agalactiae in semen and milk of goat from Pernambuco state, Brazil |
title_sort |
Mycoplasma agalactiae in semen and milk of goat from Pernambuco state, Brazil |
author |
Alves,Bruno H.L.S. |
author_facet |
Alves,Bruno H.L.S. Silva,José G. Mota,André R. Campos,Ana C. Júnior,José W.P. Santos,Sandra B. Mota,Rinaldo A. |
author_role |
author |
author2 |
Silva,José G. Mota,André R. Campos,Ana C. Júnior,José W.P. Santos,Sandra B. Mota,Rinaldo A. |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Alves,Bruno H.L.S. Silva,José G. Mota,André R. Campos,Ana C. Júnior,José W.P. Santos,Sandra B. Mota,Rinaldo A. |
dc.subject.por.fl_str_mv |
Mycoplasma agalactiae semen milk goat reproductive disorder |
topic |
Mycoplasma agalactiae semen milk goat reproductive disorder |
description |
In goat and sheep flocks, mycoplasmosis is a disease that may cause severe economical losses associated with polyarthritis, mastitis, agalactia, conjunctivitis, pneumonia and reproductive failure. The latter may involve repeat breeding, granular vulvovaginitis, infertility and abortions. The aim of the present study was to assess the occurrence of Mycoplasma agalactiae (Ma) in semen and milk samples from naturally infected goat in the semiarid region from Pernambuco State, Northeast from Brazil. Thirty-nine semen samples and 81 milk samples were submitted to DNA extraction using a commercially available kit and following the manufacturer's instructions. The polymerase chain reaction (PCR) was then performed in accordance with protocols described in the literature. The results of the present study revealed the presence of Ma in the DNA of 17.9% (7/39) of the semen samples and 3.7% (3/81) of the milk samples. The results obtained in the present study confirm the elimination of the DNA of Ma in the semen and milk samples. The presence of this agent in goat flocks is considered very risky in terms of reproductive disorders and contagious agalactia outbreaks in the Northeast region of Brazil. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-11-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2013001100004 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2013001100004 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0100-736X2013001100004 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Colégio Brasileiro de Patologia Animal - CBPA |
publisher.none.fl_str_mv |
Colégio Brasileiro de Patologia Animal - CBPA |
dc.source.none.fl_str_mv |
Pesquisa Veterinária Brasileira v.33 n.11 2013 reponame:Pesquisa Veterinária Brasileira (Online) instname:Colégio Brasileiro de Patologia Animal (CBPA) instacron:EMBRAPA |
instname_str |
Colégio Brasileiro de Patologia Animal (CBPA) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Pesquisa Veterinária Brasileira (Online) |
collection |
Pesquisa Veterinária Brasileira (Online) |
repository.name.fl_str_mv |
Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA) |
repository.mail.fl_str_mv |
colegio@cbpa.org.br||pvb@pvb.com.br |
_version_ |
1754122233704349696 |