In vitro conservation of Piper aduncum and Piper hispidinervum under slow-growth conditions

Detalhes bibliográficos
Autor(a) principal: da Silva, Tatiane Loureiro
Data de Publicação: 2011
Outros Autores: Scherwinski-Pereira, Jonny Everson
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/9758
Resumo: The objective of this work was to evaluate in vitro storage of Piper aduncum and P. hispidinervum under slow-growth conditions. Shoots were stored at low temperatures (10, 20 and 25°C), and the culture medium was supplemented with osmotic agents (sucrose and mannitol – at 1, 2 and 3%) and abiscisic acid – ABA (0, 0.5, 1.0, 2.0 and 3.0 mg L-1). After six-months of storage, shoots were evaluated for survival and regrowth. Low temperature at 20°C was effective for the in vitro conservation of P. aduncum and P. hispidinervum shoots. In vitro cultures maintained at 20°C on MS medium showed 100% survival with slow-growth shoots. The presence of mannitol or ABA, in the culture medium, negatively affected shoot growth, which is evidenced by the low rate of recovered shoots.
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spelling In vitro conservation of Piper aduncum and Piper hispidinervum under slow-growth conditionsConservação in vitro de Piper aduncum e Piper hispidinervum sob condições de crescimento lentoex situ conservation; germplasm bank; micropropagationconservação ex situ; banco do germoplasma; micropropagaçãoThe objective of this work was to evaluate in vitro storage of Piper aduncum and P. hispidinervum under slow-growth conditions. Shoots were stored at low temperatures (10, 20 and 25°C), and the culture medium was supplemented with osmotic agents (sucrose and mannitol – at 1, 2 and 3%) and abiscisic acid – ABA (0, 0.5, 1.0, 2.0 and 3.0 mg L-1). After six-months of storage, shoots were evaluated for survival and regrowth. Low temperature at 20°C was effective for the in vitro conservation of P. aduncum and P. hispidinervum shoots. In vitro cultures maintained at 20°C on MS medium showed 100% survival with slow-growth shoots. The presence of mannitol or ABA, in the culture medium, negatively affected shoot growth, which is evidenced by the low rate of recovered shoots. O objetivo deste trabalho foi avaliar a conservação in vitro de Piper aduncum e P. hispidinervum, em condições de crescimento lento. Os brotos foram armazenados a baixas temperaturas (10, 20 e 25ºC), e o meio de cultura foi suplementado com agentes osmóticos (sucrose e manitol – a 1, 2 e 3%) e ácido abcísico – ABA (0, 0,5, 1,0, 2,0 e 3,0 mg L-1). Após seis meses de armazenamentos os brotos foram avaliados quanto à sobrevivência e à rebrotação. A baixa temperatura de 20°C foi efetiva para a conservação in vitro de brotos de P. aduncum e P. hispidinervum. Culturas in vitro, mantidas a 20°C em meio MS apresentaram 100% de sobrevivência com o crescimento lento dos brotos. A presença de manitol ou ABA, no meio de cultura, afetou negativamente o crescimento de brotos, o que foi evidenciado pelo baixo índice de sobrevivência dos brotos. Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraCNPqCAPESda Silva, Tatiane LoureiroScherwinski-Pereira, Jonny Everson2011-06-22info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/9758Pesquisa Agropecuaria Brasileira; v.46, n.4, abr. 2011; 384-389Pesquisa Agropecuária Brasileira; v.46, n.4, abr. 2011; 384-3891678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAenghttps://seer.sct.embrapa.br/index.php/pab/article/view/9758/6302https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/9758/5004info:eu-repo/semantics/openAccess2014-05-23T19:00:52Zoai:ojs.seer.sct.embrapa.br:article/9758Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-05-23T19:00:52Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv In vitro conservation of Piper aduncum and Piper hispidinervum under slow-growth conditions
Conservação in vitro de Piper aduncum e Piper hispidinervum sob condições de crescimento lento
title In vitro conservation of Piper aduncum and Piper hispidinervum under slow-growth conditions
spellingShingle In vitro conservation of Piper aduncum and Piper hispidinervum under slow-growth conditions
da Silva, Tatiane Loureiro
ex situ conservation; germplasm bank; micropropagation
conservação ex situ; banco do germoplasma; micropropagação
title_short In vitro conservation of Piper aduncum and Piper hispidinervum under slow-growth conditions
title_full In vitro conservation of Piper aduncum and Piper hispidinervum under slow-growth conditions
title_fullStr In vitro conservation of Piper aduncum and Piper hispidinervum under slow-growth conditions
title_full_unstemmed In vitro conservation of Piper aduncum and Piper hispidinervum under slow-growth conditions
title_sort In vitro conservation of Piper aduncum and Piper hispidinervum under slow-growth conditions
author da Silva, Tatiane Loureiro
author_facet da Silva, Tatiane Loureiro
Scherwinski-Pereira, Jonny Everson
author_role author
author2 Scherwinski-Pereira, Jonny Everson
author2_role author
dc.contributor.none.fl_str_mv
CNPq
CAPES
dc.contributor.author.fl_str_mv da Silva, Tatiane Loureiro
Scherwinski-Pereira, Jonny Everson
dc.subject.por.fl_str_mv ex situ conservation; germplasm bank; micropropagation
conservação ex situ; banco do germoplasma; micropropagação
topic ex situ conservation; germplasm bank; micropropagation
conservação ex situ; banco do germoplasma; micropropagação
description The objective of this work was to evaluate in vitro storage of Piper aduncum and P. hispidinervum under slow-growth conditions. Shoots were stored at low temperatures (10, 20 and 25°C), and the culture medium was supplemented with osmotic agents (sucrose and mannitol – at 1, 2 and 3%) and abiscisic acid – ABA (0, 0.5, 1.0, 2.0 and 3.0 mg L-1). After six-months of storage, shoots were evaluated for survival and regrowth. Low temperature at 20°C was effective for the in vitro conservation of P. aduncum and P. hispidinervum shoots. In vitro cultures maintained at 20°C on MS medium showed 100% survival with slow-growth shoots. The presence of mannitol or ABA, in the culture medium, negatively affected shoot growth, which is evidenced by the low rate of recovered shoots.
publishDate 2011
dc.date.none.fl_str_mv 2011-06-22
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/9758
url https://seer.sct.embrapa.br/index.php/pab/article/view/9758
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/9758/6302
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/9758/5004
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.46, n.4, abr. 2011; 384-389
Pesquisa Agropecuária Brasileira; v.46, n.4, abr. 2011; 384-389
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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