Genetic diversity of piracanjuba used in stock enhancement programs with microsatellite markers

Detalhes bibliográficos
Autor(a) principal: Rodriguez-Rodriguez, Maria del Pilar
Data de Publicação: 2010
Outros Autores: Lopera-Barrero, Nelson Mauricio, Ribeiro, Ricardo Pereira, Povh, Jayme Aparecido, Vargas, Lauro, Sirol, Rodolfo Nardez, Jacometo, Carolina Bespalhok
Tipo de documento: Artigo
Idioma: spa
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/2430
Resumo: The objective of this work was to estimate the genetic diversity of a Brycon orbignyanus lot used in stock enhancement programs, using microsatellite markers. Samples of 44 broodstocks, 70 larvae and 69 fingerlings, were analyzed with amplification of five loci described for Brycon opalinus. The number of alleles, the observed (Ho) and expected (He) heterozygosity, Shannon index (IS), Nei’s genetic diversity (DGN), the inbreeding coefficient (Fis), distance (DG) and genetic identity (IG), the effective number of alleles, the test of Hardy-Weinberg equilibrium (EHW) and the linkage disequilibrium were calculated. Broodstocks and offspring had a similar number of alleles at the tested loci. Ho average, IS, DGN, DG and IG showed that there is less genetic distance between parental and larvae and a decrease of genetic variability in the fingerlings. Deviations in the EHW and disequilibrium ligation were observed in six pairs of loci. Inbreeding coefficient showed excess of heterozygotes in parental and larvae and heterozygote deficiencies in fingerlings. The broodstock is in process of allele losses and the genetic variability decreased between larva and fingerling stages.
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spelling Genetic diversity of piracanjuba used in stock enhancement programs with microsatellite markersGenetic diversity of piracanjuba used in stock enhancement programs with microsatellite markersDiversidad genética de piracanjuba usada en programas de repoblación con marcadores microsatélitesBrycon orbignyanus; fingerling; conservation genetics; larvae; reproductive management; genetic variabilityBrycon orbignyanus; fingerling; conservation genetics; larvae; reproductive management; genetic variabilityBrycon orbignyanus; alevino; genética de la conservación; larva; manejo reproductivo; variabilidad genéticaThe objective of this work was to estimate the genetic diversity of a Brycon orbignyanus lot used in stock enhancement programs, using microsatellite markers. Samples of 44 broodstocks, 70 larvae and 69 fingerlings, were analyzed with amplification of five loci described for Brycon opalinus. The number of alleles, the observed (Ho) and expected (He) heterozygosity, Shannon index (IS), Nei’s genetic diversity (DGN), the inbreeding coefficient (Fis), distance (DG) and genetic identity (IG), the effective number of alleles, the test of Hardy-Weinberg equilibrium (EHW) and the linkage disequilibrium were calculated. Broodstocks and offspring had a similar number of alleles at the tested loci. Ho average, IS, DGN, DG and IG showed that there is less genetic distance between parental and larvae and a decrease of genetic variability in the fingerlings. Deviations in the EHW and disequilibrium ligation were observed in six pairs of loci. Inbreeding coefficient showed excess of heterozygotes in parental and larvae and heterozygote deficiencies in fingerlings. The broodstock is in process of allele losses and the genetic variability decreased between larva and fingerling stages.The objective of this work was to estimate the genetic diversity of a Brycon orbignyanus lot used in stock enhancement programs, using microsatellite markers. Samples of 44 broodstocks, 70 larvae and 69 fingerlings, were analyzed with amplification of five loci described for Brycon opalinus. The number of alleles, the observed (Ho) and expected (He) heterozygosity, Shannon index (IS), Nei’s genetic diversity (DGN), the inbreeding coefficient (Fis), distance (DG) and genetic identity (IG), the effective number of alleles, the test of Hardy-Weinberg equilibrium (EHW) and the linkage disequilibrium were calculated. Broodstocks and offspring had a similar number of alleles at the tested loci. Ho average, IS, DGN, DG and IG showed that there is less genetic distance between parental and larvae and a decrease of genetic variability in the fingerlings. Deviations in the EHW and disequilibrium ligation were observed in six pairs of loci. Inbreeding coefficient showed excess of heterozygotes in parental and larvae and heterozygote deficiencies in fingerlings. The broodstock is in process of allele losses and the genetic variability decreased between larva and fingerling stages.El objetivo de este trabajo fue estimar la diversidad genética de un lote de Brycon orbignyanus usado en programas de repoblación, a través de marcadores microsatélites. Se analizaron muestras de 44 reproductores, de 70 larvas y de 69 alevinos, con la amplificación de cinco loci descritos para Brycon opalinus. El número de alelos, la heterozigosidad observada (Ho) y esperada (He), el índice de Shannon (IS), la diversidad genética de Nei (DGN), el coeficiente de endogamia (Fis), la distancia (DG) e identidad genética (IG), el número efectivo de alelos, el test del equilibrio de Hardy-Weinberg (EHW) y el desequilibrio de ligación fueron calculados. Reproductores y progenie tuvieron un número similar de alelos en los loci evaluados. La Ho media, IS, DGN, DG e IG mostraron que existe menor distancia genética entre parentales y larvas y una disminución de variabilidad genética en los alevinos. Fueron observados desvíos en EHW y desequilibrio de ligación en seis pares de loci. El Fis mostró exceso de heterocigotos en parentales y larvas y déficit de heterocigotos en los alevinos. El lote de reproductores está en proceso de pérdida de alelos y hubo disminución de la variabilidad genética entre la fase de larva y alevino.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraRodriguez-Rodriguez, Maria del PilarLopera-Barrero, Nelson MauricioRibeiro, Ricardo PereiraPovh, Jayme AparecidoVargas, LauroSirol, Rodolfo NardezJacometo, Carolina Bespalhok2010-12-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/2430Pesquisa Agropecuaria Brasileira; v.45, n.1, jan. 2010; 56-63Pesquisa Agropecuária Brasileira; v.45, n.1, jan. 2010; 56-631678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAspahttps://seer.sct.embrapa.br/index.php/pab/article/view/2430/5934https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/2430/1838https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/2430/1839info:eu-repo/semantics/openAccess2014-10-15T19:42:51Zoai:ojs.seer.sct.embrapa.br:article/2430Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-10-15T19:42:51Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Genetic diversity of piracanjuba used in stock enhancement programs with microsatellite markers
Genetic diversity of piracanjuba used in stock enhancement programs with microsatellite markers
Diversidad genética de piracanjuba usada en programas de repoblación con marcadores microsatélites
title Genetic diversity of piracanjuba used in stock enhancement programs with microsatellite markers
spellingShingle Genetic diversity of piracanjuba used in stock enhancement programs with microsatellite markers
Rodriguez-Rodriguez, Maria del Pilar
Brycon orbignyanus; fingerling; conservation genetics; larvae; reproductive management; genetic variability
Brycon orbignyanus; fingerling; conservation genetics; larvae; reproductive management; genetic variability
Brycon orbignyanus; alevino; genética de la conservación; larva; manejo reproductivo; variabilidad genética
title_short Genetic diversity of piracanjuba used in stock enhancement programs with microsatellite markers
title_full Genetic diversity of piracanjuba used in stock enhancement programs with microsatellite markers
title_fullStr Genetic diversity of piracanjuba used in stock enhancement programs with microsatellite markers
title_full_unstemmed Genetic diversity of piracanjuba used in stock enhancement programs with microsatellite markers
title_sort Genetic diversity of piracanjuba used in stock enhancement programs with microsatellite markers
author Rodriguez-Rodriguez, Maria del Pilar
author_facet Rodriguez-Rodriguez, Maria del Pilar
Lopera-Barrero, Nelson Mauricio
Ribeiro, Ricardo Pereira
Povh, Jayme Aparecido
Vargas, Lauro
Sirol, Rodolfo Nardez
Jacometo, Carolina Bespalhok
author_role author
author2 Lopera-Barrero, Nelson Mauricio
Ribeiro, Ricardo Pereira
Povh, Jayme Aparecido
Vargas, Lauro
Sirol, Rodolfo Nardez
Jacometo, Carolina Bespalhok
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv


dc.contributor.author.fl_str_mv Rodriguez-Rodriguez, Maria del Pilar
Lopera-Barrero, Nelson Mauricio
Ribeiro, Ricardo Pereira
Povh, Jayme Aparecido
Vargas, Lauro
Sirol, Rodolfo Nardez
Jacometo, Carolina Bespalhok
dc.subject.por.fl_str_mv Brycon orbignyanus; fingerling; conservation genetics; larvae; reproductive management; genetic variability
Brycon orbignyanus; fingerling; conservation genetics; larvae; reproductive management; genetic variability
Brycon orbignyanus; alevino; genética de la conservación; larva; manejo reproductivo; variabilidad genética
topic Brycon orbignyanus; fingerling; conservation genetics; larvae; reproductive management; genetic variability
Brycon orbignyanus; fingerling; conservation genetics; larvae; reproductive management; genetic variability
Brycon orbignyanus; alevino; genética de la conservación; larva; manejo reproductivo; variabilidad genética
description The objective of this work was to estimate the genetic diversity of a Brycon orbignyanus lot used in stock enhancement programs, using microsatellite markers. Samples of 44 broodstocks, 70 larvae and 69 fingerlings, were analyzed with amplification of five loci described for Brycon opalinus. The number of alleles, the observed (Ho) and expected (He) heterozygosity, Shannon index (IS), Nei’s genetic diversity (DGN), the inbreeding coefficient (Fis), distance (DG) and genetic identity (IG), the effective number of alleles, the test of Hardy-Weinberg equilibrium (EHW) and the linkage disequilibrium were calculated. Broodstocks and offspring had a similar number of alleles at the tested loci. Ho average, IS, DGN, DG and IG showed that there is less genetic distance between parental and larvae and a decrease of genetic variability in the fingerlings. Deviations in the EHW and disequilibrium ligation were observed in six pairs of loci. Inbreeding coefficient showed excess of heterozygotes in parental and larvae and heterozygote deficiencies in fingerlings. The broodstock is in process of allele losses and the genetic variability decreased between larva and fingerling stages.
publishDate 2010
dc.date.none.fl_str_mv 2010-12-28
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/2430
url https://seer.sct.embrapa.br/index.php/pab/article/view/2430
dc.language.iso.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/2430/5934
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/2430/1838
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/2430/1839
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.45, n.1, jan. 2010; 56-63
Pesquisa Agropecuária Brasileira; v.45, n.1, jan. 2010; 56-63
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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