Micropropagation of 'Trifoliata' through axiliary buds in vitro culture.

Detalhes bibliográficos
Autor(a) principal: Pasqual, Moacir
Data de Publicação: 2014
Outros Autores: Ando, Akihiko
Tipo de documento: Artigo
Idioma: por
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/14149
Resumo: Axiliary buds of Trifoliata (Poncirus trifoliata Raf.) excised from seedlings obtained in vitro were cultived on "MS" medium aditionated all of the possible combinations of NAS and BAP (0,0; 0,1; 1,0; 5.0 e 10.0 mg/l). The highest frequency of bud multiplication occurred with NAA- 10 + BAP: 1.0 mg/l and rooting with IBA from 1.0 to 2.0 mg/l or NAA-5,0 + BAP-0,1 mg/l or NAA- 1,0 + IBA-2,0 mg/l. High frequency of embryogenic callus was observed on the increased doses of BAP (up 1,0 mg/l).
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spelling Micropropagation of 'Trifoliata' through axiliary buds in vitro culture.Micropropagação de 'Trifoliata' através de cultura de gemas axilares in vitrotissues culture; citrus.cultura de tecidos; citros.Axiliary buds of Trifoliata (Poncirus trifoliata Raf.) excised from seedlings obtained in vitro were cultived on "MS" medium aditionated all of the possible combinations of NAS and BAP (0,0; 0,1; 1,0; 5.0 e 10.0 mg/l). The highest frequency of bud multiplication occurred with NAA- 10 + BAP: 1.0 mg/l and rooting with IBA from 1.0 to 2.0 mg/l or NAA-5,0 + BAP-0,1 mg/l or NAA- 1,0 + IBA-2,0 mg/l. High frequency of embryogenic callus was observed on the increased doses of BAP (up 1,0 mg/l).Gemas axilares de 'Trifoliata' (Poncirus trifoliata Raf.) excisadas de plantas obtidas através de germinação de sementes in vitro, foram cultivadas em meio básico "MS" suplementado de todas as combinações possíveis de NAA e BAP nas concentrações de 0,0; 0,1; 1,0; 5,0 e 10,0 mg/l. O melhor índice de multiplicação de gamas se deu com NAA-1,0 + BAP-1,0 mg/l e de enraizamento de brotos com IBA-1,0 a 2,0 mg/l ou NAA-5,0 + BAP-0,1 mg/l ou ainda NAA-1,0 + IBA-2, O mg/l. As doses mais elevadas de BAP (a partir de 1,0 mg/l) evidenciaram alta percentagem de explantes que formaram calos, os quais mostraram elevada capacidade de regeneração de plantas.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraPasqual, MoacirAndo, Akihiko2014-04-17info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/14149Pesquisa Agropecuaria Brasileira; v.24, n.2, fev. 1989; 217-220Pesquisa Agropecuária Brasileira; v.24, n.2, fev. 1989; 217-2201678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/14149/8092info:eu-repo/semantics/openAccess2014-08-21T19:16:04Zoai:ojs.seer.sct.embrapa.br:article/14149Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-08-21T19:16:04Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Micropropagation of 'Trifoliata' through axiliary buds in vitro culture.

Micropropagação de 'Trifoliata' através de cultura de gemas axilares in vitro
title Micropropagation of 'Trifoliata' through axiliary buds in vitro culture.
spellingShingle Micropropagation of 'Trifoliata' through axiliary buds in vitro culture.
Pasqual, Moacir
tissues culture; citrus.
cultura de tecidos; citros.
title_short Micropropagation of 'Trifoliata' through axiliary buds in vitro culture.
title_full Micropropagation of 'Trifoliata' through axiliary buds in vitro culture.
title_fullStr Micropropagation of 'Trifoliata' through axiliary buds in vitro culture.
title_full_unstemmed Micropropagation of 'Trifoliata' through axiliary buds in vitro culture.
title_sort Micropropagation of 'Trifoliata' through axiliary buds in vitro culture.
author Pasqual, Moacir
author_facet Pasqual, Moacir
Ando, Akihiko
author_role author
author2 Ando, Akihiko
author2_role author
dc.contributor.none.fl_str_mv


dc.contributor.author.fl_str_mv Pasqual, Moacir
Ando, Akihiko
dc.subject.none.fl_str_mv
dc.subject.por.fl_str_mv tissues culture; citrus.
cultura de tecidos; citros.
topic tissues culture; citrus.
cultura de tecidos; citros.
description Axiliary buds of Trifoliata (Poncirus trifoliata Raf.) excised from seedlings obtained in vitro were cultived on "MS" medium aditionated all of the possible combinations of NAS and BAP (0,0; 0,1; 1,0; 5.0 e 10.0 mg/l). The highest frequency of bud multiplication occurred with NAA- 10 + BAP: 1.0 mg/l and rooting with IBA from 1.0 to 2.0 mg/l or NAA-5,0 + BAP-0,1 mg/l or NAA- 1,0 + IBA-2,0 mg/l. High frequency of embryogenic callus was observed on the increased doses of BAP (up 1,0 mg/l).
publishDate 2014
dc.date.none.fl_str_mv 2014-04-17
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/14149
url https://seer.sct.embrapa.br/index.php/pab/article/view/14149
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/14149/8092
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.24, n.2, fev. 1989; 217-220
Pesquisa Agropecuária Brasileira; v.24, n.2, fev. 1989; 217-220
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
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institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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