Morphocultural characterization, autoinducer biosynthesis and biofilm formation in rhizobacteria isolated from vegetable crops

Detalhes bibliográficos
Autor(a) principal: Pinton, Rachel
Data de Publicação: 2011
Outros Autores: Dias, Anelise, Xavier, Terezinha Ferreira, Rouws, Luc Felicianus Marie, Xavier, Gustavo Ribeiro, Rumjanek, Norma Gouvêa, Ribeiro, Raul de Lucena Duarte
Tipo de documento: Artigo
Idioma: por
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/3066
Resumo: The objective of this work was to characterize and group rhizobacteria isolated from vegetable crops for culture morphology, richness and diversity, and to evaluate the biosynthesis of N-acyl homoserine lactone (AHL) autoinducers and the capacity to form biofilms. Seven strains were also assessed for their potential to promote plant growth of Brassica oleraceae var. acephala in greenhouse. To test the production of AHL, the indicator strain Agrobacterium tumefaciens NT1 was used. The formation of biofilms was evaluated by cultivating the isolates in liquid medium. Growth promotion was evaluated after the inoculation of the strains in kale plants and through determination of the fresh and dry mass production. The largest morphocultural diversity was found among the strains isolated from kale. From a total of 112 tested strains, 13% were positive for AHL production, among 91 strains, 96% were capable to form biofilms, and among 79 strains, 11% were positive for both characteristics. Significant differences were observed in root dry mass of plants inoculated with 109 UFC mL-1 of strain R142 that increased in 55% the root dry mass in comparison to the control. There is no relationship between the capacity to form biofilms and the detection of the AHL produced by the evaluated rhizobacteria.
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spelling Morphocultural characterization, autoinducer biosynthesis and biofilm formation in rhizobacteria isolated from vegetable cropsCaracterização morfocultural, biossíntese de autoindutor e formação de biofilme por rizobactérias de hortaliçasautoinducers; growth promotion; rhizobacteriaautoindutores; promoção de crescimento; rizobactériasThe objective of this work was to characterize and group rhizobacteria isolated from vegetable crops for culture morphology, richness and diversity, and to evaluate the biosynthesis of N-acyl homoserine lactone (AHL) autoinducers and the capacity to form biofilms. Seven strains were also assessed for their potential to promote plant growth of Brassica oleraceae var. acephala in greenhouse. To test the production of AHL, the indicator strain Agrobacterium tumefaciens NT1 was used. The formation of biofilms was evaluated by cultivating the isolates in liquid medium. Growth promotion was evaluated after the inoculation of the strains in kale plants and through determination of the fresh and dry mass production. The largest morphocultural diversity was found among the strains isolated from kale. From a total of 112 tested strains, 13% were positive for AHL production, among 91 strains, 96% were capable to form biofilms, and among 79 strains, 11% were positive for both characteristics. Significant differences were observed in root dry mass of plants inoculated with 109 UFC mL-1 of strain R142 that increased in 55% the root dry mass in comparison to the control. There is no relationship between the capacity to form biofilms and the detection of the AHL produced by the evaluated rhizobacteria.O objetivo deste trabalho foi caracterizar e agrupar rizobactérias, isoladas de hortaliças, quanto à morfologia cultural, riqueza e diversidade e avaliar a biossíntese de autoindutores N-acil lactonas homoserinas (ALH) e a capacidade de formação de biofilmes. Sete estirpes também foram avaliadas quanto ao potencial de promoção de crescimento de Brassica oleraceae var. acephala em casa de vegetação. Para verificar a produção de ALH, foram realizados ensaios com Agrobacterium tumefaciens estirpe NT1 como sistema repórter. A formação de biofilme foi avaliada pelo cultivo do isolado em meio líquido. A promoção do crescimento foi avaliada após inoculação das estirpes em plantas de couve-de-folha pela determinação da produção de massa de matérias fresca e seca. A maior diversidade morfocultural foi encontrada entre as estirpes isoladas de couve-de-folha. De um total de 112 estirpes testadas, 13% foram positivas quanto à produção de ALH; de 91 estirpes, 96% foram capazes de formar biofilmes; e de 79 estirpes, 11% foram positivas para ambas as características. Foram observadas diferenças significativas na massa de matéria seca das raízes com inoculação de 109 UFC mL-1 da estirpe R142, que incrementou em 55% a massa de matéria seca das raízes de couve, em relação ao controle. Não há relação entre a capacidade de formar biofilme e a detecção de ALH produzidos pelas rizobactérias avaliadas.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraCNPq, CAPES e FAPERJPinton, RachelDias, AneliseXavier, Terezinha FerreiraRouws, Luc Felicianus MarieXavier, Gustavo RibeiroRumjanek, Norma GouvêaRibeiro, Raul de Lucena Duarte2011-01-18info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/3066Pesquisa Agropecuaria Brasileira; v.45, n.3, mar. 2010; 284-293Pesquisa Agropecuária Brasileira; v.45, n.3, mar. 2010; 284-2931678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/3066/6017https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/3066/2598info:eu-repo/semantics/openAccess2014-10-17T16:33:06Zoai:ojs.seer.sct.embrapa.br:article/3066Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-10-17T16:33:06Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Morphocultural characterization, autoinducer biosynthesis and biofilm formation in rhizobacteria isolated from vegetable crops
Caracterização morfocultural, biossíntese de autoindutor e formação de biofilme por rizobactérias de hortaliças
title Morphocultural characterization, autoinducer biosynthesis and biofilm formation in rhizobacteria isolated from vegetable crops
spellingShingle Morphocultural characterization, autoinducer biosynthesis and biofilm formation in rhizobacteria isolated from vegetable crops
Pinton, Rachel
autoinducers; growth promotion; rhizobacteria
autoindutores; promoção de crescimento; rizobactérias
title_short Morphocultural characterization, autoinducer biosynthesis and biofilm formation in rhizobacteria isolated from vegetable crops
title_full Morphocultural characterization, autoinducer biosynthesis and biofilm formation in rhizobacteria isolated from vegetable crops
title_fullStr Morphocultural characterization, autoinducer biosynthesis and biofilm formation in rhizobacteria isolated from vegetable crops
title_full_unstemmed Morphocultural characterization, autoinducer biosynthesis and biofilm formation in rhizobacteria isolated from vegetable crops
title_sort Morphocultural characterization, autoinducer biosynthesis and biofilm formation in rhizobacteria isolated from vegetable crops
author Pinton, Rachel
author_facet Pinton, Rachel
Dias, Anelise
Xavier, Terezinha Ferreira
Rouws, Luc Felicianus Marie
Xavier, Gustavo Ribeiro
Rumjanek, Norma Gouvêa
Ribeiro, Raul de Lucena Duarte
author_role author
author2 Dias, Anelise
Xavier, Terezinha Ferreira
Rouws, Luc Felicianus Marie
Xavier, Gustavo Ribeiro
Rumjanek, Norma Gouvêa
Ribeiro, Raul de Lucena Duarte
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv
CNPq, CAPES e FAPERJ
dc.contributor.author.fl_str_mv Pinton, Rachel
Dias, Anelise
Xavier, Terezinha Ferreira
Rouws, Luc Felicianus Marie
Xavier, Gustavo Ribeiro
Rumjanek, Norma Gouvêa
Ribeiro, Raul de Lucena Duarte
dc.subject.por.fl_str_mv autoinducers; growth promotion; rhizobacteria
autoindutores; promoção de crescimento; rizobactérias
topic autoinducers; growth promotion; rhizobacteria
autoindutores; promoção de crescimento; rizobactérias
description The objective of this work was to characterize and group rhizobacteria isolated from vegetable crops for culture morphology, richness and diversity, and to evaluate the biosynthesis of N-acyl homoserine lactone (AHL) autoinducers and the capacity to form biofilms. Seven strains were also assessed for their potential to promote plant growth of Brassica oleraceae var. acephala in greenhouse. To test the production of AHL, the indicator strain Agrobacterium tumefaciens NT1 was used. The formation of biofilms was evaluated by cultivating the isolates in liquid medium. Growth promotion was evaluated after the inoculation of the strains in kale plants and through determination of the fresh and dry mass production. The largest morphocultural diversity was found among the strains isolated from kale. From a total of 112 tested strains, 13% were positive for AHL production, among 91 strains, 96% were capable to form biofilms, and among 79 strains, 11% were positive for both characteristics. Significant differences were observed in root dry mass of plants inoculated with 109 UFC mL-1 of strain R142 that increased in 55% the root dry mass in comparison to the control. There is no relationship between the capacity to form biofilms and the detection of the AHL produced by the evaluated rhizobacteria.
publishDate 2011
dc.date.none.fl_str_mv 2011-01-18
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/3066
url https://seer.sct.embrapa.br/index.php/pab/article/view/3066
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/3066/6017
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/3066/2598
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.45, n.3, mar. 2010; 284-293
Pesquisa Agropecuária Brasileira; v.45, n.3, mar. 2010; 284-293
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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