Morphocultural characterization, autoinducer biosynthesis and biofilm formation in rhizobacteria isolated from vegetable crops
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Pesquisa Agropecuária Brasileira (Online) |
Texto Completo: | https://seer.sct.embrapa.br/index.php/pab/article/view/3066 |
Resumo: | The objective of this work was to characterize and group rhizobacteria isolated from vegetable crops for culture morphology, richness and diversity, and to evaluate the biosynthesis of N-acyl homoserine lactone (AHL) autoinducers and the capacity to form biofilms. Seven strains were also assessed for their potential to promote plant growth of Brassica oleraceae var. acephala in greenhouse. To test the production of AHL, the indicator strain Agrobacterium tumefaciens NT1 was used. The formation of biofilms was evaluated by cultivating the isolates in liquid medium. Growth promotion was evaluated after the inoculation of the strains in kale plants and through determination of the fresh and dry mass production. The largest morphocultural diversity was found among the strains isolated from kale. From a total of 112 tested strains, 13% were positive for AHL production, among 91 strains, 96% were capable to form biofilms, and among 79 strains, 11% were positive for both characteristics. Significant differences were observed in root dry mass of plants inoculated with 109 UFC mL-1 of strain R142 that increased in 55% the root dry mass in comparison to the control. There is no relationship between the capacity to form biofilms and the detection of the AHL produced by the evaluated rhizobacteria. |
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Morphocultural characterization, autoinducer biosynthesis and biofilm formation in rhizobacteria isolated from vegetable cropsCaracterização morfocultural, biossíntese de autoindutor e formação de biofilme por rizobactérias de hortaliçasautoinducers; growth promotion; rhizobacteriaautoindutores; promoção de crescimento; rizobactériasThe objective of this work was to characterize and group rhizobacteria isolated from vegetable crops for culture morphology, richness and diversity, and to evaluate the biosynthesis of N-acyl homoserine lactone (AHL) autoinducers and the capacity to form biofilms. Seven strains were also assessed for their potential to promote plant growth of Brassica oleraceae var. acephala in greenhouse. To test the production of AHL, the indicator strain Agrobacterium tumefaciens NT1 was used. The formation of biofilms was evaluated by cultivating the isolates in liquid medium. Growth promotion was evaluated after the inoculation of the strains in kale plants and through determination of the fresh and dry mass production. The largest morphocultural diversity was found among the strains isolated from kale. From a total of 112 tested strains, 13% were positive for AHL production, among 91 strains, 96% were capable to form biofilms, and among 79 strains, 11% were positive for both characteristics. Significant differences were observed in root dry mass of plants inoculated with 109 UFC mL-1 of strain R142 that increased in 55% the root dry mass in comparison to the control. There is no relationship between the capacity to form biofilms and the detection of the AHL produced by the evaluated rhizobacteria.O objetivo deste trabalho foi caracterizar e agrupar rizobactérias, isoladas de hortaliças, quanto à morfologia cultural, riqueza e diversidade e avaliar a biossíntese de autoindutores N-acil lactonas homoserinas (ALH) e a capacidade de formação de biofilmes. Sete estirpes também foram avaliadas quanto ao potencial de promoção de crescimento de Brassica oleraceae var. acephala em casa de vegetação. Para verificar a produção de ALH, foram realizados ensaios com Agrobacterium tumefaciens estirpe NT1 como sistema repórter. A formação de biofilme foi avaliada pelo cultivo do isolado em meio líquido. A promoção do crescimento foi avaliada após inoculação das estirpes em plantas de couve-de-folha pela determinação da produção de massa de matérias fresca e seca. A maior diversidade morfocultural foi encontrada entre as estirpes isoladas de couve-de-folha. De um total de 112 estirpes testadas, 13% foram positivas quanto à produção de ALH; de 91 estirpes, 96% foram capazes de formar biofilmes; e de 79 estirpes, 11% foram positivas para ambas as características. Foram observadas diferenças significativas na massa de matéria seca das raízes com inoculação de 109 UFC mL-1 da estirpe R142, que incrementou em 55% a massa de matéria seca das raízes de couve, em relação ao controle. Não há relação entre a capacidade de formar biofilme e a detecção de ALH produzidos pelas rizobactérias avaliadas.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraCNPq, CAPES e FAPERJPinton, RachelDias, AneliseXavier, Terezinha FerreiraRouws, Luc Felicianus MarieXavier, Gustavo RibeiroRumjanek, Norma GouvêaRibeiro, Raul de Lucena Duarte2011-01-18info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/3066Pesquisa Agropecuaria Brasileira; v.45, n.3, mar. 2010; 284-293Pesquisa Agropecuária Brasileira; v.45, n.3, mar. 2010; 284-2931678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/3066/6017https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/3066/2598info:eu-repo/semantics/openAccess2014-10-17T16:33:06Zoai:ojs.seer.sct.embrapa.br:article/3066Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-10-17T16:33:06Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Morphocultural characterization, autoinducer biosynthesis and biofilm formation in rhizobacteria isolated from vegetable crops Caracterização morfocultural, biossíntese de autoindutor e formação de biofilme por rizobactérias de hortaliças |
title |
Morphocultural characterization, autoinducer biosynthesis and biofilm formation in rhizobacteria isolated from vegetable crops |
spellingShingle |
Morphocultural characterization, autoinducer biosynthesis and biofilm formation in rhizobacteria isolated from vegetable crops Pinton, Rachel autoinducers; growth promotion; rhizobacteria autoindutores; promoção de crescimento; rizobactérias |
title_short |
Morphocultural characterization, autoinducer biosynthesis and biofilm formation in rhizobacteria isolated from vegetable crops |
title_full |
Morphocultural characterization, autoinducer biosynthesis and biofilm formation in rhizobacteria isolated from vegetable crops |
title_fullStr |
Morphocultural characterization, autoinducer biosynthesis and biofilm formation in rhizobacteria isolated from vegetable crops |
title_full_unstemmed |
Morphocultural characterization, autoinducer biosynthesis and biofilm formation in rhizobacteria isolated from vegetable crops |
title_sort |
Morphocultural characterization, autoinducer biosynthesis and biofilm formation in rhizobacteria isolated from vegetable crops |
author |
Pinton, Rachel |
author_facet |
Pinton, Rachel Dias, Anelise Xavier, Terezinha Ferreira Rouws, Luc Felicianus Marie Xavier, Gustavo Ribeiro Rumjanek, Norma Gouvêa Ribeiro, Raul de Lucena Duarte |
author_role |
author |
author2 |
Dias, Anelise Xavier, Terezinha Ferreira Rouws, Luc Felicianus Marie Xavier, Gustavo Ribeiro Rumjanek, Norma Gouvêa Ribeiro, Raul de Lucena Duarte |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
CNPq, CAPES e FAPERJ |
dc.contributor.author.fl_str_mv |
Pinton, Rachel Dias, Anelise Xavier, Terezinha Ferreira Rouws, Luc Felicianus Marie Xavier, Gustavo Ribeiro Rumjanek, Norma Gouvêa Ribeiro, Raul de Lucena Duarte |
dc.subject.por.fl_str_mv |
autoinducers; growth promotion; rhizobacteria autoindutores; promoção de crescimento; rizobactérias |
topic |
autoinducers; growth promotion; rhizobacteria autoindutores; promoção de crescimento; rizobactérias |
description |
The objective of this work was to characterize and group rhizobacteria isolated from vegetable crops for culture morphology, richness and diversity, and to evaluate the biosynthesis of N-acyl homoserine lactone (AHL) autoinducers and the capacity to form biofilms. Seven strains were also assessed for their potential to promote plant growth of Brassica oleraceae var. acephala in greenhouse. To test the production of AHL, the indicator strain Agrobacterium tumefaciens NT1 was used. The formation of biofilms was evaluated by cultivating the isolates in liquid medium. Growth promotion was evaluated after the inoculation of the strains in kale plants and through determination of the fresh and dry mass production. The largest morphocultural diversity was found among the strains isolated from kale. From a total of 112 tested strains, 13% were positive for AHL production, among 91 strains, 96% were capable to form biofilms, and among 79 strains, 11% were positive for both characteristics. Significant differences were observed in root dry mass of plants inoculated with 109 UFC mL-1 of strain R142 that increased in 55% the root dry mass in comparison to the control. There is no relationship between the capacity to form biofilms and the detection of the AHL produced by the evaluated rhizobacteria. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-01-18 |
dc.type.none.fl_str_mv |
|
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.sct.embrapa.br/index.php/pab/article/view/3066 |
url |
https://seer.sct.embrapa.br/index.php/pab/article/view/3066 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://seer.sct.embrapa.br/index.php/pab/article/view/3066/6017 https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/3066/2598 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
publisher.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
dc.source.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira; v.45, n.3, mar. 2010; 284-293 Pesquisa Agropecuária Brasileira; v.45, n.3, mar. 2010; 284-293 1678-3921 0100-104x reponame:Pesquisa Agropecuária Brasileira (Online) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Pesquisa Agropecuária Brasileira (Online) |
collection |
Pesquisa Agropecuária Brasileira (Online) |
repository.name.fl_str_mv |
Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
pab@sct.embrapa.br || sct.pab@embrapa.br |
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1793416656816439296 |