Phenotypic diversity and symbiotic efficiency of Bradyrhizobium spp. strains from Amazonian soils

Detalhes bibliográficos
Autor(a) principal: Lima, Adriana Silva
Data de Publicação: 2005
Outros Autores: Pereira, João Paulo Andrade Resende, Moreira, Fátima Maria de Souza
Tipo de documento: Artigo
Idioma: por
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/7070
Resumo: This work aimed to evaluate the phenotypic diversity and symbiotic efficiency of Bradyrhizobium strains isolated from Amazonian soils, under different land use systems (crop, fallow, pasture, forest and agroforestry system). Total protein profiles obtained by polyacrylamide gel electrophoresis (SDS-PAGE) of 46 strains showed great diversity, while 11 groups presented similarity above 80%. One of these groups contained the reference strain of B. elkanii: BR29, recommended as soybean inoculant. Twenty-two strains, tested in Leonard jars for symbiotic efficiency with Vigna unguiculata (L.) Walp. (cowpea) produced shoot dry matter, N-content, and relative efficiency higher than the control (without N, with inoculation). Production of shoot dry matter and relative efficiency were induced by 13 strains in a way similar to the N-control (with N, without inoculation); five strains induced higher nitrogen content than N-control. Native populations comprise high diversity of strains with variable symbiotic efficiency, and some of them could be recommended for agronomic efficiency assays.
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spelling Phenotypic diversity and symbiotic efficiency of Bradyrhizobium spp. strains from Amazonian soilsDiversidade fenotípica e eficiência simbiótica de estirpes de Bradyrhizobium spp. de solos da AmazôniaVigna unguiculata; biological N2 fixation; Leguminosae nodulating bacteriaVigna unguiculata; fixação biológica de N2; bactérias nodulíferas This work aimed to evaluate the phenotypic diversity and symbiotic efficiency of Bradyrhizobium strains isolated from Amazonian soils, under different land use systems (crop, fallow, pasture, forest and agroforestry system). Total protein profiles obtained by polyacrylamide gel electrophoresis (SDS-PAGE) of 46 strains showed great diversity, while 11 groups presented similarity above 80%. One of these groups contained the reference strain of B. elkanii: BR29, recommended as soybean inoculant. Twenty-two strains, tested in Leonard jars for symbiotic efficiency with Vigna unguiculata (L.) Walp. (cowpea) produced shoot dry matter, N-content, and relative efficiency higher than the control (without N, with inoculation). Production of shoot dry matter and relative efficiency were induced by 13 strains in a way similar to the N-control (with N, without inoculation); five strains induced higher nitrogen content than N-control. Native populations comprise high diversity of strains with variable symbiotic efficiency, and some of them could be recommended for agronomic efficiency assays.Este trabalho teve como objetivo avaliar a diversidade fenotípica e a eficiência simbiótica de estirpes de Bradyrhizobium, isoladas de solos da Amazônia, sob diferentes sistemas de uso da terra (monocultura, capoeira, pastagem, floresta e sistema agroflorestal). A análise dos perfis de proteína total de 46 estirpes, obtidos por eletroforese em gel de poliacrilamida (SDS-PAGE), mostrou grande diversidade, tendo formado 11 grupos com similaridade acima de 80%. Apenas um dos grupos continha a estirpe referência de B. elkanii: BR29, recomendada como inoculante para soja. Vinte e duas estirpes testadas em vasos de Leonard, com caupi (Vigna unguiculata (L.) Walp.), induziram à produção de matéria seca e ao acúmulo de nitrogênio, na parte aérea da planta, e à eficiência relativa superiores aos da testemunha (sem N e sem inoculação). Entre as estirpes testadas, 13 induziram à produção de matéria seca e à eficiência relativa similares às da testemunha nitrogenada (com N, sem inoculação); cinco estirpes induziram a acúmulo de N superior ao da testemunha nitrogenada. Essas populações nativas são constituídas por grande diversidade de estirpes, com eficiência simbiótica variável, algumas das quais podem ser recomendadas para testes de eficiência agronômica.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraLima, Adriana SilvaPereira, João Paulo Andrade ResendeMoreira, Fátima Maria de Souza2005-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/7070Pesquisa Agropecuaria Brasileira; v.40, n.11, nov. 2005; 1095-1104Pesquisa Agropecuária Brasileira; v.40, n.11, nov. 2005; 1095-11041678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/7070/4118info:eu-repo/semantics/openAccess2014-09-19T17:40:01Zoai:ojs.seer.sct.embrapa.br:article/7070Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-09-19T17:40:01Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Phenotypic diversity and symbiotic efficiency of Bradyrhizobium spp. strains from Amazonian soils
Diversidade fenotípica e eficiência simbiótica de estirpes de Bradyrhizobium spp. de solos da Amazônia
title Phenotypic diversity and symbiotic efficiency of Bradyrhizobium spp. strains from Amazonian soils
spellingShingle Phenotypic diversity and symbiotic efficiency of Bradyrhizobium spp. strains from Amazonian soils
Lima, Adriana Silva
Vigna unguiculata; biological N2 fixation; Leguminosae nodulating bacteria
Vigna unguiculata; fixação biológica de N2; bactérias nodulíferas
title_short Phenotypic diversity and symbiotic efficiency of Bradyrhizobium spp. strains from Amazonian soils
title_full Phenotypic diversity and symbiotic efficiency of Bradyrhizobium spp. strains from Amazonian soils
title_fullStr Phenotypic diversity and symbiotic efficiency of Bradyrhizobium spp. strains from Amazonian soils
title_full_unstemmed Phenotypic diversity and symbiotic efficiency of Bradyrhizobium spp. strains from Amazonian soils
title_sort Phenotypic diversity and symbiotic efficiency of Bradyrhizobium spp. strains from Amazonian soils
author Lima, Adriana Silva
author_facet Lima, Adriana Silva
Pereira, João Paulo Andrade Resende
Moreira, Fátima Maria de Souza
author_role author
author2 Pereira, João Paulo Andrade Resende
Moreira, Fátima Maria de Souza
author2_role author
author
dc.contributor.none.fl_str_mv

dc.contributor.author.fl_str_mv Lima, Adriana Silva
Pereira, João Paulo Andrade Resende
Moreira, Fátima Maria de Souza
dc.subject.por.fl_str_mv Vigna unguiculata; biological N2 fixation; Leguminosae nodulating bacteria
Vigna unguiculata; fixação biológica de N2; bactérias nodulíferas
topic Vigna unguiculata; biological N2 fixation; Leguminosae nodulating bacteria
Vigna unguiculata; fixação biológica de N2; bactérias nodulíferas
description This work aimed to evaluate the phenotypic diversity and symbiotic efficiency of Bradyrhizobium strains isolated from Amazonian soils, under different land use systems (crop, fallow, pasture, forest and agroforestry system). Total protein profiles obtained by polyacrylamide gel electrophoresis (SDS-PAGE) of 46 strains showed great diversity, while 11 groups presented similarity above 80%. One of these groups contained the reference strain of B. elkanii: BR29, recommended as soybean inoculant. Twenty-two strains, tested in Leonard jars for symbiotic efficiency with Vigna unguiculata (L.) Walp. (cowpea) produced shoot dry matter, N-content, and relative efficiency higher than the control (without N, with inoculation). Production of shoot dry matter and relative efficiency were induced by 13 strains in a way similar to the N-control (with N, without inoculation); five strains induced higher nitrogen content than N-control. Native populations comprise high diversity of strains with variable symbiotic efficiency, and some of them could be recommended for agronomic efficiency assays.
publishDate 2005
dc.date.none.fl_str_mv 2005-11-01
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/7070
url https://seer.sct.embrapa.br/index.php/pab/article/view/7070
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/7070/4118
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.40, n.11, nov. 2005; 1095-1104
Pesquisa Agropecuária Brasileira; v.40, n.11, nov. 2005; 1095-1104
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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