Citrus asymmetric somatic hybrids produced via fusion of gamma‑irradiated and iodoacetamide‑treated protoplasts
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Pesquisa Agropecuária Brasileira (Online) |
Texto Completo: | https://seer.sct.embrapa.br/index.php/pab/article/view/1832 |
Resumo: | The objective of this study was to produce citrus somatic asymmetric hybrids by fusing gamma-irradiated protoplasts with iodoacetamide-treated protoplasts. Protoplasts were isolated from embryogenic suspension cells of grapefruit (Citrus paradisi Macfad.) cultivars Ruby Red and Flame, sweet oranges (C. sinensis Osbeck) 'Itaboraí', 'Natal', Valencia', and 'Succari', from 'Satsuma' (C. unshiu Marcow.) and 'Changsha' mandarin (C. reticulata Blanco) and 'Murcott' tangor (C. reticulata x C. sinensis). Donor protoplasts were exposed to gamma rays and receptor protoplasts were treated with 3 mmol L-1 iodoacetamide (IOA), and then they were fused for asymmetric hybridization. Asymmetric embryos were germinated, and the resulting shoots were either grafted onto sour orange, rough lemon or 'Swingle' (C. paradisi x Poncirus trifoliata) x 'Sunki' mandarin rootstock seedlings, or rooted after dipping their bases in indol-butyric acid (IBA) solution. The products were later acclimatized to greenhouse conditions. Ploidy was analyzed by flow cytometry, and hybridity was confirmed by amplified fragment length polymorphism (AFLP) analysis of plantlet DNA samples. The best treatment was the donor-recipient fusion combination of 80 Gy-irradiated 'Ruby Red' protoplasts with 20 min IOA-treated 'Succari' protoplasts. Tetraploid and aneuploid plants were produced. Rooting recalcitrance was solved by dipping shoots' stems in 3,000 mg L-1 IBA solution for 10 min. |
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Citrus asymmetric somatic hybrids produced via fusion of gamma‑irradiated and iodoacetamide‑treated protoplastsHíbridos somáticos assimétricos de citros produzidos pela fusão de protoplastos irradiados e tratados com iodoacetamidaCitrus sp.; gamma irradiation; grapefruit; protoplast fusion; somatic hybridization; sweet orangeCitrus sp.; irradiação gama; pomelo; fusão de protoplasto; hibridação somática; laranja doceThe objective of this study was to produce citrus somatic asymmetric hybrids by fusing gamma-irradiated protoplasts with iodoacetamide-treated protoplasts. Protoplasts were isolated from embryogenic suspension cells of grapefruit (Citrus paradisi Macfad.) cultivars Ruby Red and Flame, sweet oranges (C. sinensis Osbeck) 'Itaboraí', 'Natal', Valencia', and 'Succari', from 'Satsuma' (C. unshiu Marcow.) and 'Changsha' mandarin (C. reticulata Blanco) and 'Murcott' tangor (C. reticulata x C. sinensis). Donor protoplasts were exposed to gamma rays and receptor protoplasts were treated with 3 mmol L-1 iodoacetamide (IOA), and then they were fused for asymmetric hybridization. Asymmetric embryos were germinated, and the resulting shoots were either grafted onto sour orange, rough lemon or 'Swingle' (C. paradisi x Poncirus trifoliata) x 'Sunki' mandarin rootstock seedlings, or rooted after dipping their bases in indol-butyric acid (IBA) solution. The products were later acclimatized to greenhouse conditions. Ploidy was analyzed by flow cytometry, and hybridity was confirmed by amplified fragment length polymorphism (AFLP) analysis of plantlet DNA samples. The best treatment was the donor-recipient fusion combination of 80 Gy-irradiated 'Ruby Red' protoplasts with 20 min IOA-treated 'Succari' protoplasts. Tetraploid and aneuploid plants were produced. Rooting recalcitrance was solved by dipping shoots' stems in 3,000 mg L-1 IBA solution for 10 min.O objetivo deste trabalho foi produzir híbridos somáticos assimétricos de citros pela fusão de protoplastos irradiados com raios gama e protoplastos tratados com iodoacetamida. Protoplastos foram isolados de suspensões celulares embriogênicas de pomelo (Citrus paradisi Macfad.), cultivares Ruby Red e Flame, de laranja doce (C. sinensis Osbeck) 'Itaboraí', 'Natal', Valencia' e 'Succari', de tangerinas 'Satsuma' (C. unshiu Marcow.) e 'Changsha' (C. reticulata Blanco) e de tangor 'Murcott' (C. reticulata x C. sinensis). Protoplastos doadores foram expostos a raios gama e protoplastos receptores foram tratados com 3 mmol L-1 de iodoacetamida (IOA) e fusionados para hibridação assimétrica. Embriões assimétricos foram germinados e as brotações produzidas foram enxertadas sobre porta-enxertos de sementes de 'Laranja azeda', 'Limão rugoso' ou citrumelo 'Swingle' (C. paradisi x Poncirus trifoliata) x 'Sunki' ou enraizadas após embebição em solução de -- (IBA) e aclimatadas em casa de vegetação. A análise de ploidia foi realizada por meio de citometria de fluxo e a hibridicidade foi confirmada por análise de polimorfismo de comprimento de fragmentos amplificados (AFLP) de amostras de DNA das plântulas. A melhor combinação doador-recipiente foi de protoplastos de 'Ruby Red' irradiados com 80 Gy, fusionados com protoplastos de 'Succari' tratados por 20 min com IOA. Plantas tetraploides e aneuploides foram produzidas e o problema de recalcitrância ao enraizamento foi resolvido pela introdução das bases das brotações em 3.000 mg L-1 de IBA por 10 min.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraCAPESCAPESBona, Claudine Maria deGould, Jean HoweJr, J. Creighton MillerStelly, DavidLouzada, Eliezer Silva2010-11-18info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/1832Pesquisa Agropecuaria Brasileira; v.44, n.5, maio 2009; 454-462Pesquisa Agropecuária Brasileira; v.44, n.5, maio 2009; 454-4621678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/1832/5714https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/1832/1231https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/1832/1867https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/1832/1894info:eu-repo/semantics/openAccess2012-06-17T11:49:50Zoai:ojs.seer.sct.embrapa.br:article/1832Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2012-06-17T11:49:50Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Citrus asymmetric somatic hybrids produced via fusion of gamma‑irradiated and iodoacetamide‑treated protoplasts Híbridos somáticos assimétricos de citros produzidos pela fusão de protoplastos irradiados e tratados com iodoacetamida |
title |
Citrus asymmetric somatic hybrids produced via fusion of gamma‑irradiated and iodoacetamide‑treated protoplasts |
spellingShingle |
Citrus asymmetric somatic hybrids produced via fusion of gamma‑irradiated and iodoacetamide‑treated protoplasts Bona, Claudine Maria de Citrus sp.; gamma irradiation; grapefruit; protoplast fusion; somatic hybridization; sweet orange Citrus sp.; irradiação gama; pomelo; fusão de protoplasto; hibridação somática; laranja doce |
title_short |
Citrus asymmetric somatic hybrids produced via fusion of gamma‑irradiated and iodoacetamide‑treated protoplasts |
title_full |
Citrus asymmetric somatic hybrids produced via fusion of gamma‑irradiated and iodoacetamide‑treated protoplasts |
title_fullStr |
Citrus asymmetric somatic hybrids produced via fusion of gamma‑irradiated and iodoacetamide‑treated protoplasts |
title_full_unstemmed |
Citrus asymmetric somatic hybrids produced via fusion of gamma‑irradiated and iodoacetamide‑treated protoplasts |
title_sort |
Citrus asymmetric somatic hybrids produced via fusion of gamma‑irradiated and iodoacetamide‑treated protoplasts |
author |
Bona, Claudine Maria de |
author_facet |
Bona, Claudine Maria de Gould, Jean Howe Jr, J. Creighton Miller Stelly, David Louzada, Eliezer Silva |
author_role |
author |
author2 |
Gould, Jean Howe Jr, J. Creighton Miller Stelly, David Louzada, Eliezer Silva |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
CAPES CAPES |
dc.contributor.author.fl_str_mv |
Bona, Claudine Maria de Gould, Jean Howe Jr, J. Creighton Miller Stelly, David Louzada, Eliezer Silva |
dc.subject.por.fl_str_mv |
Citrus sp.; gamma irradiation; grapefruit; protoplast fusion; somatic hybridization; sweet orange Citrus sp.; irradiação gama; pomelo; fusão de protoplasto; hibridação somática; laranja doce |
topic |
Citrus sp.; gamma irradiation; grapefruit; protoplast fusion; somatic hybridization; sweet orange Citrus sp.; irradiação gama; pomelo; fusão de protoplasto; hibridação somática; laranja doce |
description |
The objective of this study was to produce citrus somatic asymmetric hybrids by fusing gamma-irradiated protoplasts with iodoacetamide-treated protoplasts. Protoplasts were isolated from embryogenic suspension cells of grapefruit (Citrus paradisi Macfad.) cultivars Ruby Red and Flame, sweet oranges (C. sinensis Osbeck) 'Itaboraí', 'Natal', Valencia', and 'Succari', from 'Satsuma' (C. unshiu Marcow.) and 'Changsha' mandarin (C. reticulata Blanco) and 'Murcott' tangor (C. reticulata x C. sinensis). Donor protoplasts were exposed to gamma rays and receptor protoplasts were treated with 3 mmol L-1 iodoacetamide (IOA), and then they were fused for asymmetric hybridization. Asymmetric embryos were germinated, and the resulting shoots were either grafted onto sour orange, rough lemon or 'Swingle' (C. paradisi x Poncirus trifoliata) x 'Sunki' mandarin rootstock seedlings, or rooted after dipping their bases in indol-butyric acid (IBA) solution. The products were later acclimatized to greenhouse conditions. Ploidy was analyzed by flow cytometry, and hybridity was confirmed by amplified fragment length polymorphism (AFLP) analysis of plantlet DNA samples. The best treatment was the donor-recipient fusion combination of 80 Gy-irradiated 'Ruby Red' protoplasts with 20 min IOA-treated 'Succari' protoplasts. Tetraploid and aneuploid plants were produced. Rooting recalcitrance was solved by dipping shoots' stems in 3,000 mg L-1 IBA solution for 10 min. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-11-18 |
dc.type.none.fl_str_mv |
|
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.sct.embrapa.br/index.php/pab/article/view/1832 |
url |
https://seer.sct.embrapa.br/index.php/pab/article/view/1832 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://seer.sct.embrapa.br/index.php/pab/article/view/1832/5714 https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/1832/1231 https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/1832/1867 https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/1832/1894 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
publisher.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
dc.source.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira; v.44, n.5, maio 2009; 454-462 Pesquisa Agropecuária Brasileira; v.44, n.5, maio 2009; 454-462 1678-3921 0100-104x reponame:Pesquisa Agropecuária Brasileira (Online) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Pesquisa Agropecuária Brasileira (Online) |
collection |
Pesquisa Agropecuária Brasileira (Online) |
repository.name.fl_str_mv |
Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
pab@sct.embrapa.br || sct.pab@embrapa.br |
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1793416661353627648 |