Survival of sugarcane shoot tips after cryopreservation by droplet-vitrification

Detalhes bibliográficos
Autor(a) principal: Nogueira, Gabriela Ferreira
Data de Publicação: 2014
Outros Autores: Pasqual, Moacir, Scherwinski-Pereira, Jonny Everson
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/17488
Resumo: The objective of this work was to evaluate the phytotoxicity of a plant vitrification solution (PVS2), and the survival of shoot tips of the sugarcane variety SP716949, after cryopreservation by droplet‑vitrification. Shoot tips were precultured for 24 hours in MS medium containing 0.3 mol L-1 sucrose, and exposed to PVS2 for 0, 20 or 30 min. Shoot tips were then immersed in liquid nitrogen. Thawing was fast in concentrated sucrose solution (1.2 mol L-1). PVS2 is a nontoxic to shoot tips, which in turn are sensitive to liquid nitrogen. The best results occurred when shoot tips were maintained for up to 20 min in PVS2 solution, before freezing, with 20% survival.
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spelling Survival of sugarcane shoot tips after cryopreservation by droplet-vitrificationSobrevivência de ápices caulinares de cana‑de‑açúcar após criopreservação por “droplet-vitrification”Saccharum; in vitro conservation; PVS2; regenerationSaccharum; conservação in vitro; PVS2; regeneraçãoThe objective of this work was to evaluate the phytotoxicity of a plant vitrification solution (PVS2), and the survival of shoot tips of the sugarcane variety SP716949, after cryopreservation by droplet‑vitrification. Shoot tips were precultured for 24 hours in MS medium containing 0.3 mol L-1 sucrose, and exposed to PVS2 for 0, 20 or 30 min. Shoot tips were then immersed in liquid nitrogen. Thawing was fast in concentrated sucrose solution (1.2 mol L-1). PVS2 is a nontoxic to shoot tips, which in turn are sensitive to liquid nitrogen. The best results occurred when shoot tips were maintained for up to 20 min in PVS2 solution, before freezing, with 20% survival.O objetivo deste trabalho foi avaliar a fitotoxicidade da solução de vitrificação de plantas (PVS2) e a sobrevivência de ápices caulinares de cana‑de‑açúcar, variedade SP716949, após criopreservação por “droplet‑vitrification”. Ápices caulinares foram pré‑cultivados por 24 horas em meio MS com sacarose a 0,3 mol L-1 e expostos por 0, 20 ou 30 min a PVS2. Em seguida, os ápices foram mergulhados em nitrogênio líquido. O descongelamento em solução concentrada de sacarose (1,2 mol L-1) foi rápido. A PVS2 não tem efeito tóxico aos ápices que, no entanto, são sensíveis ao nitrogênio líquido. O melhor resultado ocorreu, quando os ápices foram mantidos por 20 min na solução PVS2, antes do congelamento, com 20% de sobrevivência.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraEmbrapa, CNPq, CapesNogueira, Gabriela FerreiraPasqual, MoacirScherwinski-Pereira, Jonny Everson2014-02-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/17488Pesquisa Agropecuaria Brasileira; v.48, n.11, nov. 2013; 1524-1527Pesquisa Agropecuária Brasileira; v.48, n.11, nov. 2013; 1524-15271678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAenghttps://seer.sct.embrapa.br/index.php/pab/article/view/17488/12463info:eu-repo/semantics/openAccess2014-02-11T19:52:57Zoai:ojs.seer.sct.embrapa.br:article/17488Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-02-11T19:52:57Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Survival of sugarcane shoot tips after cryopreservation by droplet-vitrification
Sobrevivência de ápices caulinares de cana‑de‑açúcar após criopreservação por “droplet-vitrification”
title Survival of sugarcane shoot tips after cryopreservation by droplet-vitrification
spellingShingle Survival of sugarcane shoot tips after cryopreservation by droplet-vitrification
Nogueira, Gabriela Ferreira
Saccharum; in vitro conservation; PVS2; regeneration
Saccharum; conservação in vitro; PVS2; regeneração
title_short Survival of sugarcane shoot tips after cryopreservation by droplet-vitrification
title_full Survival of sugarcane shoot tips after cryopreservation by droplet-vitrification
title_fullStr Survival of sugarcane shoot tips after cryopreservation by droplet-vitrification
title_full_unstemmed Survival of sugarcane shoot tips after cryopreservation by droplet-vitrification
title_sort Survival of sugarcane shoot tips after cryopreservation by droplet-vitrification
author Nogueira, Gabriela Ferreira
author_facet Nogueira, Gabriela Ferreira
Pasqual, Moacir
Scherwinski-Pereira, Jonny Everson
author_role author
author2 Pasqual, Moacir
Scherwinski-Pereira, Jonny Everson
author2_role author
author
dc.contributor.none.fl_str_mv Embrapa, CNPq, Capes
dc.contributor.author.fl_str_mv Nogueira, Gabriela Ferreira
Pasqual, Moacir
Scherwinski-Pereira, Jonny Everson
dc.subject.por.fl_str_mv Saccharum; in vitro conservation; PVS2; regeneration
Saccharum; conservação in vitro; PVS2; regeneração
topic Saccharum; in vitro conservation; PVS2; regeneration
Saccharum; conservação in vitro; PVS2; regeneração
description The objective of this work was to evaluate the phytotoxicity of a plant vitrification solution (PVS2), and the survival of shoot tips of the sugarcane variety SP716949, after cryopreservation by droplet‑vitrification. Shoot tips were precultured for 24 hours in MS medium containing 0.3 mol L-1 sucrose, and exposed to PVS2 for 0, 20 or 30 min. Shoot tips were then immersed in liquid nitrogen. Thawing was fast in concentrated sucrose solution (1.2 mol L-1). PVS2 is a nontoxic to shoot tips, which in turn are sensitive to liquid nitrogen. The best results occurred when shoot tips were maintained for up to 20 min in PVS2 solution, before freezing, with 20% survival.
publishDate 2014
dc.date.none.fl_str_mv 2014-02-11
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/17488
url https://seer.sct.embrapa.br/index.php/pab/article/view/17488
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/17488/12463
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.48, n.11, nov. 2013; 1524-1527
Pesquisa Agropecuária Brasileira; v.48, n.11, nov. 2013; 1524-1527
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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