Effects of different sucrose and mineral salt concentrations on in vitro propagation of Nephrolepis exaltata. an ornamental fern

Detalhes bibliográficos
Autor(a) principal: Pasqual, Moacir
Data de Publicação: 1994
Outros Autores: Hoshika, Edna, Ishida, Jorge Susumu
Tipo de documento: Artigo
Idioma: por
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/4222
Resumo: The purpose of the present work was to make possible the rapid propagation of ornamental fern (Nephrolepis exaltata) by tissues culture techniques. The explants used were shoots from in vitro plants. All the treatments consisted of combinations of mineral salts of 'MS' medium (0,0; 25,0; 50,0; 75,0 and 100,0%) and sucrose (0; 15; 30; 45 and 100 g/l) concentrations. The best results were obtained in mineral salts 25% and sucrose 30 g/l, producing 2,6 new shoots. Sucrose 15 - 30 g/l had the best number of leaves and the mineral salt concentrations did not effect that character. It was also observed that higher levels of mineral salts and sucrose reduced the growth of leaves.
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spelling Effects of different sucrose and mineral salt concentrations on in vitro propagation of Nephrolepis exaltata. an ornamental fernInfluência de diferentes concentrações de sacarose e sais minerais sobre a multiplicação in vitro de Nephrolepis exaltata. uma samambaia ornamentaltissue culture; micropropagationcultura de tecidos; micropropagaçãoThe purpose of the present work was to make possible the rapid propagation of ornamental fern (Nephrolepis exaltata) by tissues culture techniques. The explants used were shoots from in vitro plants. All the treatments consisted of combinations of mineral salts of 'MS' medium (0,0; 25,0; 50,0; 75,0 and 100,0%) and sucrose (0; 15; 30; 45 and 100 g/l) concentrations. The best results were obtained in mineral salts 25% and sucrose 30 g/l, producing 2,6 new shoots. Sucrose 15 - 30 g/l had the best number of leaves and the mineral salt concentrations did not effect that character. It was also observed that higher levels of mineral salts and sucrose reduced the growth of leaves.Objetivou-se, com o presente trabalho, viabilizar a propagação rápida de diversas samambaias ornamentais (Nephrolepis exaltata) através da técnica de cultura de tecidos. Os explantes utilizados foram brotações obtidas a partir do cultivo in vitro de estolhos terminais. Os tratamentos constaram de combinações entre concentrações de sais do meio básico de Murashige & Skoog (1962) - MS (0, 25, 50, 75 a 100%) e sacarose (0, 15, 30, 45 e 60 g/l). Os melhores resultados quanto a número de brotações foram obtidos na presença de 25% de sais e 30 g/l de sacarose, produzindo 2,6 novas brotações. Dosagens de sacarose entre 15 e 30 g/l maximizaram a produção de folhas, e a concentração de sais não teve efeito significativo neste parâmetro. Observou-se, ainda, que taxas mais elevadas de sacarose e sais inibiram o crescimento das folhas produzidas.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraPasqual, MoacirHoshika, EdnaIshida, Jorge Susumu1994-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/4222Pesquisa Agropecuaria Brasileira; v.29, n.11, nov. 1994; 1681-1684Pesquisa Agropecuária Brasileira; v.29, n.11, nov. 1994; 1681-16841678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/4222/1513info:eu-repo/semantics/openAccess2010-04-09T12:10:47Zoai:ojs.seer.sct.embrapa.br:article/4222Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2010-04-09T12:10:47Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Effects of different sucrose and mineral salt concentrations on in vitro propagation of Nephrolepis exaltata. an ornamental fern
Influência de diferentes concentrações de sacarose e sais minerais sobre a multiplicação in vitro de Nephrolepis exaltata. uma samambaia ornamental
title Effects of different sucrose and mineral salt concentrations on in vitro propagation of Nephrolepis exaltata. an ornamental fern
spellingShingle Effects of different sucrose and mineral salt concentrations on in vitro propagation of Nephrolepis exaltata. an ornamental fern
Pasqual, Moacir
tissue culture; micropropagation
cultura de tecidos; micropropagação
title_short Effects of different sucrose and mineral salt concentrations on in vitro propagation of Nephrolepis exaltata. an ornamental fern
title_full Effects of different sucrose and mineral salt concentrations on in vitro propagation of Nephrolepis exaltata. an ornamental fern
title_fullStr Effects of different sucrose and mineral salt concentrations on in vitro propagation of Nephrolepis exaltata. an ornamental fern
title_full_unstemmed Effects of different sucrose and mineral salt concentrations on in vitro propagation of Nephrolepis exaltata. an ornamental fern
title_sort Effects of different sucrose and mineral salt concentrations on in vitro propagation of Nephrolepis exaltata. an ornamental fern
author Pasqual, Moacir
author_facet Pasqual, Moacir
Hoshika, Edna
Ishida, Jorge Susumu
author_role author
author2 Hoshika, Edna
Ishida, Jorge Susumu
author2_role author
author
dc.contributor.none.fl_str_mv

dc.contributor.author.fl_str_mv Pasqual, Moacir
Hoshika, Edna
Ishida, Jorge Susumu
dc.subject.por.fl_str_mv tissue culture; micropropagation
cultura de tecidos; micropropagação
topic tissue culture; micropropagation
cultura de tecidos; micropropagação
description The purpose of the present work was to make possible the rapid propagation of ornamental fern (Nephrolepis exaltata) by tissues culture techniques. The explants used were shoots from in vitro plants. All the treatments consisted of combinations of mineral salts of 'MS' medium (0,0; 25,0; 50,0; 75,0 and 100,0%) and sucrose (0; 15; 30; 45 and 100 g/l) concentrations. The best results were obtained in mineral salts 25% and sucrose 30 g/l, producing 2,6 new shoots. Sucrose 15 - 30 g/l had the best number of leaves and the mineral salt concentrations did not effect that character. It was also observed that higher levels of mineral salts and sucrose reduced the growth of leaves.
publishDate 1994
dc.date.none.fl_str_mv 1994-11-01
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/4222
url https://seer.sct.embrapa.br/index.php/pab/article/view/4222
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/4222/1513
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.29, n.11, nov. 1994; 1681-1684
Pesquisa Agropecuária Brasileira; v.29, n.11, nov. 1994; 1681-1684
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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