Somatic embryogenesis inanthurium (Anthurium andraeanum cv. Eidibel) as affected by different explants

• Autor(a) principal: Pinheiro, Marcos Vinícius Marques
• Data de Publicação: 2014
• Outros Autores: Cruz, Ana Claudia Ferreira da, Oliveira, Evelyn Jardim de, Otoni, Wagner Campos, Martins, Fabrina Bolzan, Carvalho, Ana Cristina Portugal Pinto de
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: http://dx.doi.org/10.4025/actasciagron.v36i1.16557; http://www.locus.ufv.br/handle/123456789/25472
• topic: Auxins; Nodal segments; Somatic embryos; Auxinas; Segmentos nodais; Embriões somáticos

This study establishes a protocol for the induction of somatic embryogenesis in Anthurium andraeanum cv. Eidibel. The experiment was arranged in a completely randomized 5 x 5 x 5 factorial design using five explant types (whole leaves, half leaves; petiole; nodal segments and root segments) from in vitro plantlets; five auxins: indole-3-acetic acid (IAA), α-naphthaleneacetic acid (NAA), indole-3-butyric acid (IBA), 2,4-dichlorophenoxyacetic acid (2,4-D), and 4-amino-3,5,6-trichloropicolinic acid (Picloram); at five concentrations (0, 2.5, 5, 7.5 and 10 µM), with five replications using five Petri dishes. The cultures were maintained in a growth room at 25 ± 2ºC in the dark. The explant type was investigated for the induction of somatic embryogenesis in anthurium cv. Eidibel, and nodal segments were shown to be the most suitable explant for this process. After 60 days in culture, the highest number of embryogenic calli was recorded for the nodal segments cultured in NAA (5, 7.5 and 10 µM), 2,4-D (10 µM) and Picloram