Incidence of Garlic common latent virus in Argentina, and phylogenetic and recombination analyses of isolates
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Pesquisa Agropecuária Brasileira (Online) |
Texto Completo: | https://seer.sct.embrapa.br/index.php/pab/article/view/20498 |
Resumo: | The objective of this work was to estimate the incidence and prevalence of Garlic common latent virus (GarCLV) in the main production regions of garlic (Allium sativum) in Argentina, and to perform phylogenetic and recombination analyses in isolates from these regions. Leaf samples (3,050) were taken from four garlic commercial types, in 13 departments of the four main garlic‑producing provinces of Argentina, in a 1,175‑ha sampling area. Virus infection was evaluated with DAS‑Elisa test using specific antiserum, and the phylogenetic and recombination analyses were done with capsid protein (CP) nucleotide sequence of seven GarCLV isolates from the provinces. The incidence of GarCLV in the evaluated provinces varied between 6.7 and 22% of the samples, whereas the prevalence varied between 52.6 and 70%. In the analysis of garlic commercial types, Morado showed the highest incidence of the virus, in the province of San Juan, whereas Rosado Paraguayo had the lowest incidence, in the province of Cordoba. Nucleotide identity in the CP sequences ranged between 80.3 and 97.6%. The phylogenetic analysis shows the presence of two main groups of GarCLV and of a possible third group that would include only a German isolate. The recombination analysis between isolates from different parts of the world evidences the presence of recombinant isolates from Poland and Australia. |
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Incidence of Garlic common latent virus in Argentina, and phylogenetic and recombination analyses of isolatesIncidência de Garlic common latent virus na Argentina, e análise filogenética e de recombinação de isoladosAllium sativum; Carlavirus; GarCLV; prevalence; viral diversityAllium sativum; Carlavirus; GarCLV; prevalência; diversidade viralThe objective of this work was to estimate the incidence and prevalence of Garlic common latent virus (GarCLV) in the main production regions of garlic (Allium sativum) in Argentina, and to perform phylogenetic and recombination analyses in isolates from these regions. Leaf samples (3,050) were taken from four garlic commercial types, in 13 departments of the four main garlic‑producing provinces of Argentina, in a 1,175‑ha sampling area. Virus infection was evaluated with DAS‑Elisa test using specific antiserum, and the phylogenetic and recombination analyses were done with capsid protein (CP) nucleotide sequence of seven GarCLV isolates from the provinces. The incidence of GarCLV in the evaluated provinces varied between 6.7 and 22% of the samples, whereas the prevalence varied between 52.6 and 70%. In the analysis of garlic commercial types, Morado showed the highest incidence of the virus, in the province of San Juan, whereas Rosado Paraguayo had the lowest incidence, in the province of Cordoba. Nucleotide identity in the CP sequences ranged between 80.3 and 97.6%. The phylogenetic analysis shows the presence of two main groups of GarCLV and of a possible third group that would include only a German isolate. The recombination analysis between isolates from different parts of the world evidences the presence of recombinant isolates from Poland and Australia.O objetivo deste trabalho foi estimar a incidência e a prevalência de Garlic common latent virus (GarCLV) nas principais regiões produtoras de alho (Allium sativum) da Argentina, e realizar análises filogenética e de recombinação de isolados dessas regiões. Amostras foliares (3.050) foram coletadas de quatro tipos comerciais de alho, em 13 departamentos, nas quatro principais províncias produtoras de alho na Argentina, em uma área amostrada de 1.175 ha. A infecção viral foi avaliada pelo teste DAS‑Elisa com antissoro específico, e as análises filogenética e de recombinação foram feitas com sequências de nucleotídeos da proteína capsidial (CP) de sete isolados das províncias. A incidência de GarCLV nas províncias avaliadas variou de 6,7 a 22% das amostras, enquanto a prevalência variou de 52,6 a 70%. Na avaliação dos tipos comerciais de alho, Morado apresentou as maiores incidências do vírus, na Província de San Juan, enquanto Rosado Paraguayo apresentou a menor incidência, na Província de Córdoba. A identidade de nucleotídeos nas sequências de CP variou entre 80,3 e 97,6%. A análise filogenética mostra a presença de dois grupos principais de GarCLV e de um possível terceiro grupo formado por apenas um isolado da Alemanha. A análise de recombinação entre isolados de diferentes partes do mundo evidencia a presença de isolados recombinantes da Polônia e da Austrália.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraINTA and CONICETTorrico, Ada KarinaCelli, Marcos GiovaniConci, Luis RogelioConci, Vilma Cecilia2015-05-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/20498Pesquisa Agropecuaria Brasileira; v.50, n.5, maio 2015; 363-373Pesquisa Agropecuária Brasileira; v.50, n.5, maio 2015; 363-3731678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAenghttps://seer.sct.embrapa.br/index.php/pab/article/view/20498/12928https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/20498/12679info:eu-repo/semantics/openAccess2015-05-27T20:56:49Zoai:ojs.seer.sct.embrapa.br:article/20498Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2015-05-27T20:56:49Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Incidence of Garlic common latent virus in Argentina, and phylogenetic and recombination analyses of isolates Incidência de Garlic common latent virus na Argentina, e análise filogenética e de recombinação de isolados |
title |
Incidence of Garlic common latent virus in Argentina, and phylogenetic and recombination analyses of isolates |
spellingShingle |
Incidence of Garlic common latent virus in Argentina, and phylogenetic and recombination analyses of isolates Torrico, Ada Karina Allium sativum; Carlavirus; GarCLV; prevalence; viral diversity Allium sativum; Carlavirus; GarCLV; prevalência; diversidade viral |
title_short |
Incidence of Garlic common latent virus in Argentina, and phylogenetic and recombination analyses of isolates |
title_full |
Incidence of Garlic common latent virus in Argentina, and phylogenetic and recombination analyses of isolates |
title_fullStr |
Incidence of Garlic common latent virus in Argentina, and phylogenetic and recombination analyses of isolates |
title_full_unstemmed |
Incidence of Garlic common latent virus in Argentina, and phylogenetic and recombination analyses of isolates |
title_sort |
Incidence of Garlic common latent virus in Argentina, and phylogenetic and recombination analyses of isolates |
author |
Torrico, Ada Karina |
author_facet |
Torrico, Ada Karina Celli, Marcos Giovani Conci, Luis Rogelio Conci, Vilma Cecilia |
author_role |
author |
author2 |
Celli, Marcos Giovani Conci, Luis Rogelio Conci, Vilma Cecilia |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
INTA and CONICET |
dc.contributor.author.fl_str_mv |
Torrico, Ada Karina Celli, Marcos Giovani Conci, Luis Rogelio Conci, Vilma Cecilia |
dc.subject.por.fl_str_mv |
Allium sativum; Carlavirus; GarCLV; prevalence; viral diversity Allium sativum; Carlavirus; GarCLV; prevalência; diversidade viral |
topic |
Allium sativum; Carlavirus; GarCLV; prevalence; viral diversity Allium sativum; Carlavirus; GarCLV; prevalência; diversidade viral |
description |
The objective of this work was to estimate the incidence and prevalence of Garlic common latent virus (GarCLV) in the main production regions of garlic (Allium sativum) in Argentina, and to perform phylogenetic and recombination analyses in isolates from these regions. Leaf samples (3,050) were taken from four garlic commercial types, in 13 departments of the four main garlic‑producing provinces of Argentina, in a 1,175‑ha sampling area. Virus infection was evaluated with DAS‑Elisa test using specific antiserum, and the phylogenetic and recombination analyses were done with capsid protein (CP) nucleotide sequence of seven GarCLV isolates from the provinces. The incidence of GarCLV in the evaluated provinces varied between 6.7 and 22% of the samples, whereas the prevalence varied between 52.6 and 70%. In the analysis of garlic commercial types, Morado showed the highest incidence of the virus, in the province of San Juan, whereas Rosado Paraguayo had the lowest incidence, in the province of Cordoba. Nucleotide identity in the CP sequences ranged between 80.3 and 97.6%. The phylogenetic analysis shows the presence of two main groups of GarCLV and of a possible third group that would include only a German isolate. The recombination analysis between isolates from different parts of the world evidences the presence of recombinant isolates from Poland and Australia. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-05-06 |
dc.type.none.fl_str_mv |
|
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.sct.embrapa.br/index.php/pab/article/view/20498 |
url |
https://seer.sct.embrapa.br/index.php/pab/article/view/20498 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://seer.sct.embrapa.br/index.php/pab/article/view/20498/12928 https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/20498/12679 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
publisher.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
dc.source.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira; v.50, n.5, maio 2015; 363-373 Pesquisa Agropecuária Brasileira; v.50, n.5, maio 2015; 363-373 1678-3921 0100-104x reponame:Pesquisa Agropecuária Brasileira (Online) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Pesquisa Agropecuária Brasileira (Online) |
collection |
Pesquisa Agropecuária Brasileira (Online) |
repository.name.fl_str_mv |
Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
pab@sct.embrapa.br || sct.pab@embrapa.br |
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1793416716319981568 |