In vitro rooting and acclimatization of Corymbia citriodora seedlings
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Pesquisa Florestal Brasileira (Online) |
Texto Completo: | https://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/2232 |
Resumo: | The objective of this study was to evaluate the effect of indolbutyric acid (IBA) concentrations on in vitro rooting of Corymbia citriodora juvenile shoots, and sealing forms on the acclimatization of micropropagated seedlings. For rooting, shoots were placed in a culture medium with four concentrations of IBA (0, 1.0, 2.0, and 4.0 mg L-1). In pre-acclimatization, seedlings germinated in vitro and without contamination were transplanted into polyethylene cups containing substrate. The cups were packed in plastic bags for 10 days and kept in a culture room. Subsequently, three treatments were applied: no coverage; intact plastic bag, and perforated plastic bag. In acclimatization, the history of pre-acclimatization treatments was maintained. The seedlings were transplanted into tubes containing substrate and kept initially in a greenhouse and then in a shade house. The results demonstrate that concentrations of 1.0; 2.0 and 4.0 mg L-1 of IBA were efficient in the rooting of juvenile explants of C. citriodora, and the highest concentration used provided the highest average number of roots. The absence of cover in pre-acclimatization is recommended for greater survival rate, height, and dry mass of seedlings in the acclimatization stage. |
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In vitro rooting and acclimatization of Corymbia citriodora seedlingsEnraizamento in vitro e aclimatização de plântulas de Corymbia citriodoraCultura de tecidosÁcido indolbutíricoVedaçãoTissue cultureIndolebutyric acidSealThe objective of this study was to evaluate the effect of indolbutyric acid (IBA) concentrations on in vitro rooting of Corymbia citriodora juvenile shoots, and sealing forms on the acclimatization of micropropagated seedlings. For rooting, shoots were placed in a culture medium with four concentrations of IBA (0, 1.0, 2.0, and 4.0 mg L-1). In pre-acclimatization, seedlings germinated in vitro and without contamination were transplanted into polyethylene cups containing substrate. The cups were packed in plastic bags for 10 days and kept in a culture room. Subsequently, three treatments were applied: no coverage; intact plastic bag, and perforated plastic bag. In acclimatization, the history of pre-acclimatization treatments was maintained. The seedlings were transplanted into tubes containing substrate and kept initially in a greenhouse and then in a shade house. The results demonstrate that concentrations of 1.0; 2.0 and 4.0 mg L-1 of IBA were efficient in the rooting of juvenile explants of C. citriodora, and the highest concentration used provided the highest average number of roots. The absence of cover in pre-acclimatization is recommended for greater survival rate, height, and dry mass of seedlings in the acclimatization stage.O objetivo do presente trabalho foi avaliar o efeito de concentrações de ácido indolbutírico (AIB) no enraizamento in vitro de brotações juvenis de Corymbia citriodora, e formas de vedação na aclimatização de plântulas micropropagadas. Para o enraizamento, foram colocadas brotações em meio de cultura com quatro concentrações de AIB (0; 1,0; 2,0 e 4,0 mg L-1). Na pré-aclimatização, plântulas germinadas in vitro e isentas de contaminação foram transplantadas para copos de polietileno contendo substrato. Os copos foram embalados em sacos plásticos por 10 dias e mantidos em sala de cultura. Posteriormente, foram aplicados três tratamentos: ausência de cobertura; saco plástico intacto e saco plástico perfurado. Na aclimatização, manteve-se o histórico dos tratamentos da pré-aclimatização. As plântulas foram transplantadas para tubetes contendo substrato e mantidas inicialmente em casa de vegetação e depois em casa de sombra. Os resultados demonstram que as concentrações de 1,0; 2,0 e 4,0 mg L-1 de AIB foram eficientes no enraizamento de explantes juvenis de C. citriodora, sendo que a maior concentração utilizada proporcionou o maior número médio de raízes. Recomenda-se a ausência de cobertura na pré-aclimatização para maior taxa de sobrevivência, altura e massa seca das mudas na etapa de aclimatização.Embrapa Florestas2023-05-18info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/octet-streamhttps://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/223210.4336/2023.pfb.43e202102232Pesquisa Florestal Brasileira; v. 43 (2023)Pesquisa Florestal Brasileira; Vol. 43 (2023)1983-26051809-3647reponame:Pesquisa Florestal Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/2232/1693Copyright (c) 2023 Luiz Filipe Maravilha, Miranda Titon, Vitória de Souza Canguçu, Fabiana Miranda Rocha, Marcio Leles Romarco de Oliveirahttps://creativecommons.org/licenses/by-nc-nd/4.0info:eu-repo/semantics/openAccessMaravilha, Luiz Filipe Titon, MirandaCanguçu, Vitória de SouzaRocha, Fabiana MirandaOliveira, Marcio Leles Romarco de2023-03-16T12:47:49Zoai:pfb.cnpf.embrapa.br/pfb:article/2232Revistahttps://pfb.cnpf.embrapa.br/pfb/index.php/pfb/PUBhttps://pfb.cnpf.embrapa.br/pfb/index.php/pfb/oaipfb@embrapa.br || revista.pfb@gmail.com || patricia.mattos@embrapa.br1983-26051809-3647opendoar:2023-03-16T12:47:49Pesquisa Florestal Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
In vitro rooting and acclimatization of Corymbia citriodora seedlings Enraizamento in vitro e aclimatização de plântulas de Corymbia citriodora |
title |
In vitro rooting and acclimatization of Corymbia citriodora seedlings |
spellingShingle |
In vitro rooting and acclimatization of Corymbia citriodora seedlings Maravilha, Luiz Filipe Cultura de tecidos Ácido indolbutírico Vedação Tissue culture Indolebutyric acid Seal |
title_short |
In vitro rooting and acclimatization of Corymbia citriodora seedlings |
title_full |
In vitro rooting and acclimatization of Corymbia citriodora seedlings |
title_fullStr |
In vitro rooting and acclimatization of Corymbia citriodora seedlings |
title_full_unstemmed |
In vitro rooting and acclimatization of Corymbia citriodora seedlings |
title_sort |
In vitro rooting and acclimatization of Corymbia citriodora seedlings |
author |
Maravilha, Luiz Filipe |
author_facet |
Maravilha, Luiz Filipe Titon, Miranda Canguçu, Vitória de Souza Rocha, Fabiana Miranda Oliveira, Marcio Leles Romarco de |
author_role |
author |
author2 |
Titon, Miranda Canguçu, Vitória de Souza Rocha, Fabiana Miranda Oliveira, Marcio Leles Romarco de |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Maravilha, Luiz Filipe Titon, Miranda Canguçu, Vitória de Souza Rocha, Fabiana Miranda Oliveira, Marcio Leles Romarco de |
dc.subject.por.fl_str_mv |
Cultura de tecidos Ácido indolbutírico Vedação Tissue culture Indolebutyric acid Seal |
topic |
Cultura de tecidos Ácido indolbutírico Vedação Tissue culture Indolebutyric acid Seal |
description |
The objective of this study was to evaluate the effect of indolbutyric acid (IBA) concentrations on in vitro rooting of Corymbia citriodora juvenile shoots, and sealing forms on the acclimatization of micropropagated seedlings. For rooting, shoots were placed in a culture medium with four concentrations of IBA (0, 1.0, 2.0, and 4.0 mg L-1). In pre-acclimatization, seedlings germinated in vitro and without contamination were transplanted into polyethylene cups containing substrate. The cups were packed in plastic bags for 10 days and kept in a culture room. Subsequently, three treatments were applied: no coverage; intact plastic bag, and perforated plastic bag. In acclimatization, the history of pre-acclimatization treatments was maintained. The seedlings were transplanted into tubes containing substrate and kept initially in a greenhouse and then in a shade house. The results demonstrate that concentrations of 1.0; 2.0 and 4.0 mg L-1 of IBA were efficient in the rooting of juvenile explants of C. citriodora, and the highest concentration used provided the highest average number of roots. The absence of cover in pre-acclimatization is recommended for greater survival rate, height, and dry mass of seedlings in the acclimatization stage. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-05-18 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/2232 10.4336/2023.pfb.43e202102232 |
url |
https://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/2232 |
identifier_str_mv |
10.4336/2023.pfb.43e202102232 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/2232/1693 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by-nc-nd/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-nd/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/octet-stream |
dc.publisher.none.fl_str_mv |
Embrapa Florestas |
publisher.none.fl_str_mv |
Embrapa Florestas |
dc.source.none.fl_str_mv |
Pesquisa Florestal Brasileira; v. 43 (2023) Pesquisa Florestal Brasileira; Vol. 43 (2023) 1983-2605 1809-3647 reponame:Pesquisa Florestal Brasileira (Online) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Pesquisa Florestal Brasileira (Online) |
collection |
Pesquisa Florestal Brasileira (Online) |
repository.name.fl_str_mv |
Pesquisa Florestal Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
pfb@embrapa.br || revista.pfb@gmail.com || patricia.mattos@embrapa.br |
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1783370931802996736 |