Association Mapping Considering Allele Dosage: An Example of Forage Traits in an Interspecific Segmental Allotetraploid Urochloa spp.
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1117774 |
Resumo: | The breeding process in tropical segmental allopolyploid forage Urochloa is challenging due to the complex genetic control of the traits. Knowledge about genes associated with forage traits, expressed in the different cutting seasons, are extremely useful to support breeding programs and development of new cultivars. Thus, the aims of our study were (i) to identify genomic regions related to forage traits through genomewide association studies (GWAS), and (ii) to verify the influence of allele dosage on these results. A panel of 272 genotypes of Urochloa spp. [U. brizantha (Hoscht. ex A. Rich.) R. Webster ´ U. ruziziensis (Hoscht. ex A. Rich.) R. Webster] was evaluated in both the wet and dry seasons. The GWAS analyses were performed with 26,535 single nucleotide polymorphisms (SNPs) obtained by genotyping-by-sequencing (GBS) using diploid and tetraploid allele dosage configurations. Furthermore, we evaluated scenarios including additive, dominance, and epistatic effects. Seven candidate genomic regions associated with the main forage traits of Urochloa spp. were identified. The importance of the diploid and tetraploid molecular configuration in GWAS analyses for segmental allopolyploid species was demonstrated to identify the genomic behavior of important regions. Results demonstrated that it is possible to identify the same regions using both ploidy configurations; however, in some cases, the allele substitution effect can be biased mainly for regions with dominance and epistatic effects. Finally, this study contributes to the understanding of genetic control of tropical forage traits and genomics to accelerate the selection and reduce the cost to release new cultivars. |
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Association Mapping Considering Allele Dosage: An Example of Forage Traits in an Interspecific Segmental Allotetraploid Urochloa spp.Tropical regionsUrochloaForageAnimal proteinsCattle feedingAnimal performancePasturesThe breeding process in tropical segmental allopolyploid forage Urochloa is challenging due to the complex genetic control of the traits. Knowledge about genes associated with forage traits, expressed in the different cutting seasons, are extremely useful to support breeding programs and development of new cultivars. Thus, the aims of our study were (i) to identify genomic regions related to forage traits through genomewide association studies (GWAS), and (ii) to verify the influence of allele dosage on these results. A panel of 272 genotypes of Urochloa spp. [U. brizantha (Hoscht. ex A. Rich.) R. Webster ´ U. ruziziensis (Hoscht. ex A. Rich.) R. Webster] was evaluated in both the wet and dry seasons. The GWAS analyses were performed with 26,535 single nucleotide polymorphisms (SNPs) obtained by genotyping-by-sequencing (GBS) using diploid and tetraploid allele dosage configurations. Furthermore, we evaluated scenarios including additive, dominance, and epistatic effects. Seven candidate genomic regions associated with the main forage traits of Urochloa spp. were identified. The importance of the diploid and tetraploid molecular configuration in GWAS analyses for segmental allopolyploid species was demonstrated to identify the genomic behavior of important regions. Results demonstrated that it is possible to identify the same regions using both ploidy configurations; however, in some cases, the allele substitution effect can be biased mainly for regions with dominance and epistatic effects. Finally, this study contributes to the understanding of genetic control of tropical forage traits and genomics to accelerate the selection and reduce the cost to release new cultivars.Filipe Inácio Matias, Universidade de São Paulo - USP/Escola Superior de Agricultura "Luiz de Queiroz" - ESALQ/Departamento de Genética; Miriam Suzane Vidotti, Universidade de São Paulo - USP/Escola Superior de Agricultura "Luiz de Queiroz" - ESALQ/Departamento de Genética; KAREM GUIMARAES XAVIER MEIRELES, CNPGC; SANZIO CARVALHO LIMA BARRIOS, CNPGC; Cacilda Borges do Valle, Colaboradora da Embrapa Gado de Corte; Cari A. Schmitz Carley; Roberto Fritsche-Neto, Universidade de São Paulo - USP/Escola Superior de Agricultura "Luiz de Queiroz" - ESALQ/Departamento de GenéticaUniversidade de São Paulo - USP/Escola Superior de Agricultura "Luiz de Queiroz" - ESALQ/Departamento de Genética.MATIAS, F. I.VIDOTTI, M. S.MEIRELES, K. G. X.BARRIOS, S. C. L.VALLE, C. B. doCARLEY, C. A. S.FRITSCHE-NETO, R.2019-12-27T18:16:04Z2019-12-27T18:16:04Z2019-12-2720192019-12-27T18:16:04Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleCrop Science, v. 59, n. 5, p. 2062-2076, september-october, 2019.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1117774enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2019-12-27T18:16:12Zoai:www.alice.cnptia.embrapa.br:doc/1117774Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542019-12-27T18:16:12falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542019-12-27T18:16:12Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Association Mapping Considering Allele Dosage: An Example of Forage Traits in an Interspecific Segmental Allotetraploid Urochloa spp. |
title |
Association Mapping Considering Allele Dosage: An Example of Forage Traits in an Interspecific Segmental Allotetraploid Urochloa spp. |
spellingShingle |
Association Mapping Considering Allele Dosage: An Example of Forage Traits in an Interspecific Segmental Allotetraploid Urochloa spp. MATIAS, F. I. Tropical regions Urochloa Forage Animal proteins Cattle feeding Animal performance Pastures |
title_short |
Association Mapping Considering Allele Dosage: An Example of Forage Traits in an Interspecific Segmental Allotetraploid Urochloa spp. |
title_full |
Association Mapping Considering Allele Dosage: An Example of Forage Traits in an Interspecific Segmental Allotetraploid Urochloa spp. |
title_fullStr |
Association Mapping Considering Allele Dosage: An Example of Forage Traits in an Interspecific Segmental Allotetraploid Urochloa spp. |
title_full_unstemmed |
Association Mapping Considering Allele Dosage: An Example of Forage Traits in an Interspecific Segmental Allotetraploid Urochloa spp. |
title_sort |
Association Mapping Considering Allele Dosage: An Example of Forage Traits in an Interspecific Segmental Allotetraploid Urochloa spp. |
author |
MATIAS, F. I. |
author_facet |
MATIAS, F. I. VIDOTTI, M. S. MEIRELES, K. G. X. BARRIOS, S. C. L. VALLE, C. B. do CARLEY, C. A. S. FRITSCHE-NETO, R. |
author_role |
author |
author2 |
VIDOTTI, M. S. MEIRELES, K. G. X. BARRIOS, S. C. L. VALLE, C. B. do CARLEY, C. A. S. FRITSCHE-NETO, R. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Filipe Inácio Matias, Universidade de São Paulo - USP/Escola Superior de Agricultura "Luiz de Queiroz" - ESALQ/Departamento de Genética; Miriam Suzane Vidotti, Universidade de São Paulo - USP/Escola Superior de Agricultura "Luiz de Queiroz" - ESALQ/Departamento de Genética; KAREM GUIMARAES XAVIER MEIRELES, CNPGC; SANZIO CARVALHO LIMA BARRIOS, CNPGC; Cacilda Borges do Valle, Colaboradora da Embrapa Gado de Corte; Cari A. Schmitz Carley; Roberto Fritsche-Neto, Universidade de São Paulo - USP/Escola Superior de Agricultura "Luiz de Queiroz" - ESALQ/Departamento de GenéticaUniversidade de São Paulo - USP/Escola Superior de Agricultura "Luiz de Queiroz" - ESALQ/Departamento de Genética. |
dc.contributor.author.fl_str_mv |
MATIAS, F. I. VIDOTTI, M. S. MEIRELES, K. G. X. BARRIOS, S. C. L. VALLE, C. B. do CARLEY, C. A. S. FRITSCHE-NETO, R. |
dc.subject.por.fl_str_mv |
Tropical regions Urochloa Forage Animal proteins Cattle feeding Animal performance Pastures |
topic |
Tropical regions Urochloa Forage Animal proteins Cattle feeding Animal performance Pastures |
description |
The breeding process in tropical segmental allopolyploid forage Urochloa is challenging due to the complex genetic control of the traits. Knowledge about genes associated with forage traits, expressed in the different cutting seasons, are extremely useful to support breeding programs and development of new cultivars. Thus, the aims of our study were (i) to identify genomic regions related to forage traits through genomewide association studies (GWAS), and (ii) to verify the influence of allele dosage on these results. A panel of 272 genotypes of Urochloa spp. [U. brizantha (Hoscht. ex A. Rich.) R. Webster ´ U. ruziziensis (Hoscht. ex A. Rich.) R. Webster] was evaluated in both the wet and dry seasons. The GWAS analyses were performed with 26,535 single nucleotide polymorphisms (SNPs) obtained by genotyping-by-sequencing (GBS) using diploid and tetraploid allele dosage configurations. Furthermore, we evaluated scenarios including additive, dominance, and epistatic effects. Seven candidate genomic regions associated with the main forage traits of Urochloa spp. were identified. The importance of the diploid and tetraploid molecular configuration in GWAS analyses for segmental allopolyploid species was demonstrated to identify the genomic behavior of important regions. Results demonstrated that it is possible to identify the same regions using both ploidy configurations; however, in some cases, the allele substitution effect can be biased mainly for regions with dominance and epistatic effects. Finally, this study contributes to the understanding of genetic control of tropical forage traits and genomics to accelerate the selection and reduce the cost to release new cultivars. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-12-27T18:16:04Z 2019-12-27T18:16:04Z 2019-12-27 2019 2019-12-27T18:16:04Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Crop Science, v. 59, n. 5, p. 2062-2076, september-october, 2019. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1117774 |
identifier_str_mv |
Crop Science, v. 59, n. 5, p. 2062-2076, september-october, 2019. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1117774 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
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Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
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EMBRAPA |
institution |
EMBRAPA |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503486682955776 |