Bacterial cellulose production by Komagataeibacter hansenii ATCC 23769 using sisal juice: an agroindustry waste.

• Autor(a) principal: LIMA, H. L. S.
• Data de Publicação: 2017
• Outros Autores: NASCIMENTO, E. S., ANDRADE, F. K., BRIGIDA, A. I. S., BORGES, M. de F., CASSALES, A. R., MUNIZ, C. R., SOUZA FILHO, M. de S. M. de, MORAIS, J. P. S., ROSA, M. de F.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
• Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1087796
• topic: Celulose bacteriana; Komagataeibacter hansenii; Nanotecnologia; Resíduo agroindustrial; Sisal; nanotechnology

In this study, we have optimized production of bacterial cellulose (BC) by Komagataeibacter hansenii ATCC 23769 in a static cultivation using sisal juice, an agroindustrial residue, as substrate. Optimization of fermentation parameters has been carried out using the one-variable-at-a-time method. Effect of initial sugar concentration, pH, nitrogen supplement, and cultivation time was evaluated. The influence of nitrogen source and quantity for bacterial cellulose production was studied using a central composite rotational design (CCRD).The highest production of BC (3.38 g/L) was obtained after 10 days of cultivation, using sisal juice (pH 5) at 15 g/L of sugars and supplemented with 7.5 g/L of extract yeast. The cellulose production yield in selected sisal culture conditions was three times higher than the yield in synthetic medium, indicating that sisal juice is a suitable substrate for BC production.