Genetic similarities of Escherichia coli isolated from different substrates of the broiler production chain. Brazilian Journal of Poultry Science, v. 23, n. 1, 2021.
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1133298 http://dx.doi.org/10.1590/1806-9061-2020-1361 |
Resumo: | Abstract: Brazil is the largest exporter of chicken meat and poultry farming is one of the most important productive segments, despite major losses due to the bacterium Escherichia coli, which is also a zoonotic microorganism. The objetive of this study was to isolate E. coli and to evaluate its transmissibility potential from the field to chicken meat using the Pulsed Field Gel Electrophoresis (PFGE) technique. Environmental samples (poultry litter, soil and water) were collected from broiler farms located in the South of Brazil where the majority of the Brazilian poultry production occurs. In addition, chicken meat (gizzard, heart, drumette and tulip) samples were collected from local supermarkets. As results, 47.36% of the samples were positives for E. coli. Furthermore, 10 pairs of clones of E. coli were found always in the same substrate (two waterwater pairs; three soil-soil pairs and five meat-meat pairs) using PFGE. These findings suggest that certain strains of E. coli may have habitat preferences, making the transfer from one substrate type to another more difficult to occur. Moreover, since no clones were found between environmental samples and chicken meat, it is possible to imply a low risk of E. coli transmissibility throughout the chicken meat production chain. |
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Genetic similarities of Escherichia coli isolated from different substrates of the broiler production chain. Brazilian Journal of Poultry Science, v. 23, n. 1, 2021.Pulsed field gel electrophoresisProdução AnimalFrango de CorteSalmonellaEscherichia ColiIsolamentoEpidemiologiaMicrobiologiaSaúde PúblicaMeio AmbienteEnvironmentEpidemiologyMicrobiologyPublic healthAbstract: Brazil is the largest exporter of chicken meat and poultry farming is one of the most important productive segments, despite major losses due to the bacterium Escherichia coli, which is also a zoonotic microorganism. The objetive of this study was to isolate E. coli and to evaluate its transmissibility potential from the field to chicken meat using the Pulsed Field Gel Electrophoresis (PFGE) technique. Environmental samples (poultry litter, soil and water) were collected from broiler farms located in the South of Brazil where the majority of the Brazilian poultry production occurs. In addition, chicken meat (gizzard, heart, drumette and tulip) samples were collected from local supermarkets. As results, 47.36% of the samples were positives for E. coli. Furthermore, 10 pairs of clones of E. coli were found always in the same substrate (two waterwater pairs; three soil-soil pairs and five meat-meat pairs) using PFGE. These findings suggest that certain strains of E. coli may have habitat preferences, making the transfer from one substrate type to another more difficult to occur. Moreover, since no clones were found between environmental samples and chicken meat, it is possible to imply a low risk of E. coli transmissibility throughout the chicken meat production chain.UDESC/Chapecó; MARCEL MANENTE BOIAGO, UDESC/Chapecó; UDESC/Chapecó; UDESC/Chapecó; UDESC/Chapecó; JALUSA DEON KICH, CNPSA; RAQUEL REBELATTO, CNPSA; TALES Q. FURIAN, UFRGS; UDESC/Florianópolis.TELES, P. F. S.BOIAGO, M. M.FRIGO, A.RAMPAZZO, L.ARAÚJO, D. N.KICH, J. D.REBELATTO, R.FURIAN, T. Q.STEFANI, L. M.2021-08-07T02:04:35Z2021-08-07T02:04:35Z2021-08-062021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleBrazilian Journal of Poultry Science=Revista Brasileira de Ciência Avícola, v. 23, n. 1, 2021.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1133298http://dx.doi.org/10.1590/1806-9061-2020-1361enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2021-08-07T02:04:43Zoai:www.alice.cnptia.embrapa.br:doc/1133298Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542021-08-07T02:04:43falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542021-08-07T02:04:43Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Genetic similarities of Escherichia coli isolated from different substrates of the broiler production chain. Brazilian Journal of Poultry Science, v. 23, n. 1, 2021. |
title |
Genetic similarities of Escherichia coli isolated from different substrates of the broiler production chain. Brazilian Journal of Poultry Science, v. 23, n. 1, 2021. |
spellingShingle |
Genetic similarities of Escherichia coli isolated from different substrates of the broiler production chain. Brazilian Journal of Poultry Science, v. 23, n. 1, 2021. TELES, P. F. S. Pulsed field gel electrophoresis Produção Animal Frango de Corte Salmonella Escherichia Coli Isolamento Epidemiologia Microbiologia Saúde Pública Meio Ambiente Environment Epidemiology Microbiology Public health |
title_short |
Genetic similarities of Escherichia coli isolated from different substrates of the broiler production chain. Brazilian Journal of Poultry Science, v. 23, n. 1, 2021. |
title_full |
Genetic similarities of Escherichia coli isolated from different substrates of the broiler production chain. Brazilian Journal of Poultry Science, v. 23, n. 1, 2021. |
title_fullStr |
Genetic similarities of Escherichia coli isolated from different substrates of the broiler production chain. Brazilian Journal of Poultry Science, v. 23, n. 1, 2021. |
title_full_unstemmed |
Genetic similarities of Escherichia coli isolated from different substrates of the broiler production chain. Brazilian Journal of Poultry Science, v. 23, n. 1, 2021. |
title_sort |
Genetic similarities of Escherichia coli isolated from different substrates of the broiler production chain. Brazilian Journal of Poultry Science, v. 23, n. 1, 2021. |
author |
TELES, P. F. S. |
author_facet |
TELES, P. F. S. BOIAGO, M. M. FRIGO, A. RAMPAZZO, L. ARAÚJO, D. N. KICH, J. D. REBELATTO, R. FURIAN, T. Q. STEFANI, L. M. |
author_role |
author |
author2 |
BOIAGO, M. M. FRIGO, A. RAMPAZZO, L. ARAÚJO, D. N. KICH, J. D. REBELATTO, R. FURIAN, T. Q. STEFANI, L. M. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
UDESC/Chapecó; MARCEL MANENTE BOIAGO, UDESC/Chapecó; UDESC/Chapecó; UDESC/Chapecó; UDESC/Chapecó; JALUSA DEON KICH, CNPSA; RAQUEL REBELATTO, CNPSA; TALES Q. FURIAN, UFRGS; UDESC/Florianópolis. |
dc.contributor.author.fl_str_mv |
TELES, P. F. S. BOIAGO, M. M. FRIGO, A. RAMPAZZO, L. ARAÚJO, D. N. KICH, J. D. REBELATTO, R. FURIAN, T. Q. STEFANI, L. M. |
dc.subject.por.fl_str_mv |
Pulsed field gel electrophoresis Produção Animal Frango de Corte Salmonella Escherichia Coli Isolamento Epidemiologia Microbiologia Saúde Pública Meio Ambiente Environment Epidemiology Microbiology Public health |
topic |
Pulsed field gel electrophoresis Produção Animal Frango de Corte Salmonella Escherichia Coli Isolamento Epidemiologia Microbiologia Saúde Pública Meio Ambiente Environment Epidemiology Microbiology Public health |
description |
Abstract: Brazil is the largest exporter of chicken meat and poultry farming is one of the most important productive segments, despite major losses due to the bacterium Escherichia coli, which is also a zoonotic microorganism. The objetive of this study was to isolate E. coli and to evaluate its transmissibility potential from the field to chicken meat using the Pulsed Field Gel Electrophoresis (PFGE) technique. Environmental samples (poultry litter, soil and water) were collected from broiler farms located in the South of Brazil where the majority of the Brazilian poultry production occurs. In addition, chicken meat (gizzard, heart, drumette and tulip) samples were collected from local supermarkets. As results, 47.36% of the samples were positives for E. coli. Furthermore, 10 pairs of clones of E. coli were found always in the same substrate (two waterwater pairs; three soil-soil pairs and five meat-meat pairs) using PFGE. These findings suggest that certain strains of E. coli may have habitat preferences, making the transfer from one substrate type to another more difficult to occur. Moreover, since no clones were found between environmental samples and chicken meat, it is possible to imply a low risk of E. coli transmissibility throughout the chicken meat production chain. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-08-07T02:04:35Z 2021-08-07T02:04:35Z 2021-08-06 2021 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Brazilian Journal of Poultry Science=Revista Brasileira de Ciência Avícola, v. 23, n. 1, 2021. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1133298 http://dx.doi.org/10.1590/1806-9061-2020-1361 |
identifier_str_mv |
Brazilian Journal of Poultry Science=Revista Brasileira de Ciência Avícola, v. 23, n. 1, 2021. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1133298 http://dx.doi.org/10.1590/1806-9061-2020-1361 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
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Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
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EMBRAPA |
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EMBRAPA |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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