Genetic Similarities of Escherichia Coli Isolated from Different Substrates of the Broiler Production Chain
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Poultry Science (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2021000100308 |
Resumo: | ABSTRACT Brazil is the largest exporter of chicken meat and poultry farming is one of the most important productive segments, despite major losses due to the bacterium Escherichia coli, which is also a zoonotic microorganism. The objetive of this study was to isolate E. coli and to evaluate its transmissibility potential from the field to chicken meat using the Pulsed Field Gel Electrophoresis (PFGE) technique. Environmental samples (poultry litter, soil and water) were collected from broiler farms located in the South of Brazil where the majority of the Brazilian poultry production occurs. In addition, chicken meat (gizzard, heart, drumette and tulip) samples were collected from local supermarkets. As results, 47.36% of the samples were positives for E. coli. Furthermore, 10 pairs of clones of E. coli were found always in the same substrate (two water-water pairs; three soil-soil pairs and five meat-meat pairs) using PFGE. These findings suggest that certain strains of E. coli may have habitat preferences, making the transfer from one substrate type to another more difficult to occur. Moreover, since no clones were found between environmental samples and chicken meat, it is possible to imply a low risk of E. coli transmissibility throughout the chicken meat production chain. |
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Genetic Similarities of Escherichia Coli Isolated from Different Substrates of the Broiler Production ChainEnvironmentepidemiologymicrobiologypublic healthpulsed field gel electrophoresisABSTRACT Brazil is the largest exporter of chicken meat and poultry farming is one of the most important productive segments, despite major losses due to the bacterium Escherichia coli, which is also a zoonotic microorganism. The objetive of this study was to isolate E. coli and to evaluate its transmissibility potential from the field to chicken meat using the Pulsed Field Gel Electrophoresis (PFGE) technique. Environmental samples (poultry litter, soil and water) were collected from broiler farms located in the South of Brazil where the majority of the Brazilian poultry production occurs. In addition, chicken meat (gizzard, heart, drumette and tulip) samples were collected from local supermarkets. As results, 47.36% of the samples were positives for E. coli. Furthermore, 10 pairs of clones of E. coli were found always in the same substrate (two water-water pairs; three soil-soil pairs and five meat-meat pairs) using PFGE. These findings suggest that certain strains of E. coli may have habitat preferences, making the transfer from one substrate type to another more difficult to occur. Moreover, since no clones were found between environmental samples and chicken meat, it is possible to imply a low risk of E. coli transmissibility throughout the chicken meat production chain.Fundacao de Apoio a Ciência e Tecnologia Avicolas2021-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2021000100308Brazilian Journal of Poultry Science v.23 n.1 2021reponame:Brazilian Journal of Poultry Science (Online)instname:Fundação APINCO de Ciência e Tecnologia Avícolas (FACTA)instacron:FACTA10.1590/1806-9061-2020-1361info:eu-repo/semantics/openAccessTeles,PFSBoiago,MMFrigo,ARampazzo,LAraújo,DNKich,JDRebelatto,RFurian,TQStefani,LMeng2021-02-19T00:00:00Zoai:scielo:S1516-635X2021000100308Revistahttp://www.scielo.br/rbcahttps://old.scielo.br/oai/scielo-oai.php||rvfacta@terra.com.br1806-90611516-635Xopendoar:2021-02-19T00:00Brazilian Journal of Poultry Science (Online) - Fundação APINCO de Ciência e Tecnologia Avícolas (FACTA)false |
dc.title.none.fl_str_mv |
Genetic Similarities of Escherichia Coli Isolated from Different Substrates of the Broiler Production Chain |
title |
Genetic Similarities of Escherichia Coli Isolated from Different Substrates of the Broiler Production Chain |
spellingShingle |
Genetic Similarities of Escherichia Coli Isolated from Different Substrates of the Broiler Production Chain Teles,PFS Environment epidemiology microbiology public health pulsed field gel electrophoresis |
title_short |
Genetic Similarities of Escherichia Coli Isolated from Different Substrates of the Broiler Production Chain |
title_full |
Genetic Similarities of Escherichia Coli Isolated from Different Substrates of the Broiler Production Chain |
title_fullStr |
Genetic Similarities of Escherichia Coli Isolated from Different Substrates of the Broiler Production Chain |
title_full_unstemmed |
Genetic Similarities of Escherichia Coli Isolated from Different Substrates of the Broiler Production Chain |
title_sort |
Genetic Similarities of Escherichia Coli Isolated from Different Substrates of the Broiler Production Chain |
author |
Teles,PFS |
author_facet |
Teles,PFS Boiago,MM Frigo,A Rampazzo,L Araújo,DN Kich,JD Rebelatto,R Furian,TQ Stefani,LM |
author_role |
author |
author2 |
Boiago,MM Frigo,A Rampazzo,L Araújo,DN Kich,JD Rebelatto,R Furian,TQ Stefani,LM |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Teles,PFS Boiago,MM Frigo,A Rampazzo,L Araújo,DN Kich,JD Rebelatto,R Furian,TQ Stefani,LM |
dc.subject.por.fl_str_mv |
Environment epidemiology microbiology public health pulsed field gel electrophoresis |
topic |
Environment epidemiology microbiology public health pulsed field gel electrophoresis |
description |
ABSTRACT Brazil is the largest exporter of chicken meat and poultry farming is one of the most important productive segments, despite major losses due to the bacterium Escherichia coli, which is also a zoonotic microorganism. The objetive of this study was to isolate E. coli and to evaluate its transmissibility potential from the field to chicken meat using the Pulsed Field Gel Electrophoresis (PFGE) technique. Environmental samples (poultry litter, soil and water) were collected from broiler farms located in the South of Brazil where the majority of the Brazilian poultry production occurs. In addition, chicken meat (gizzard, heart, drumette and tulip) samples were collected from local supermarkets. As results, 47.36% of the samples were positives for E. coli. Furthermore, 10 pairs of clones of E. coli were found always in the same substrate (two water-water pairs; three soil-soil pairs and five meat-meat pairs) using PFGE. These findings suggest that certain strains of E. coli may have habitat preferences, making the transfer from one substrate type to another more difficult to occur. Moreover, since no clones were found between environmental samples and chicken meat, it is possible to imply a low risk of E. coli transmissibility throughout the chicken meat production chain. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2021000100308 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2021000100308 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1806-9061-2020-1361 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Fundacao de Apoio a Ciência e Tecnologia Avicolas |
publisher.none.fl_str_mv |
Fundacao de Apoio a Ciência e Tecnologia Avicolas |
dc.source.none.fl_str_mv |
Brazilian Journal of Poultry Science v.23 n.1 2021 reponame:Brazilian Journal of Poultry Science (Online) instname:Fundação APINCO de Ciência e Tecnologia Avícolas (FACTA) instacron:FACTA |
instname_str |
Fundação APINCO de Ciência e Tecnologia Avícolas (FACTA) |
instacron_str |
FACTA |
institution |
FACTA |
reponame_str |
Brazilian Journal of Poultry Science (Online) |
collection |
Brazilian Journal of Poultry Science (Online) |
repository.name.fl_str_mv |
Brazilian Journal of Poultry Science (Online) - Fundação APINCO de Ciência e Tecnologia Avícolas (FACTA) |
repository.mail.fl_str_mv |
||rvfacta@terra.com.br |
_version_ |
1754122515641270272 |