Genetic similarities of Escherichia coli isolated from different substrates of the broiler production chain
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/232972 |
Resumo: | Brazil is the largest exporter of chicken meat and poultry farming is one of the most important productive segments, despite major losses due to the bacterium Escherichia coli, which is also a zoonotic microorganism. The objetive of this study was to isolate E. coli and to evaluate its transmissibility potential from the field to chicken meat using the Pulsed Field Gel Electrophoresis (PFGE) technique. Environmental samples (poultry litter, soil and water) were collected from broiler farms located in the South of Brazil where the majority of the Brazilian poultry production occurs. In addition, chicken meat (gizzard, heart, drumette and tulip) samples were collected from local supermarkets. As results, 47.36% of the samples were positives for E. coli. Furthermore, 10 pairs of clones of E. coli were found always in the same substrate (two waterwater pairs; three soil-soil pairs and five meat-meat pairs) using PFGE. These findings suggest that certain strains of E. coli may have habitat preferences, making the transfer from one substrate type to another more difficult to occur. Moreover, since no clones were found between environmental samples and chicken meat, it is possible to imply a low risk of E. coli transmissibility throughout the chicken meat production chain. |
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Teles, Pedro Filipe de SouzaBoiago, Marcel ManenteFrigo, AngélicaRampazzo, LuanaAraujo, Denise NunesKich, Jalusa DeonRebelatto, RaquelFurian, Thales QuediStefani, Lenita de Cássia Moura2021-12-15T04:26:20Z20211516-635Xhttp://hdl.handle.net/10183/232972001134010Brazil is the largest exporter of chicken meat and poultry farming is one of the most important productive segments, despite major losses due to the bacterium Escherichia coli, which is also a zoonotic microorganism. The objetive of this study was to isolate E. coli and to evaluate its transmissibility potential from the field to chicken meat using the Pulsed Field Gel Electrophoresis (PFGE) technique. Environmental samples (poultry litter, soil and water) were collected from broiler farms located in the South of Brazil where the majority of the Brazilian poultry production occurs. In addition, chicken meat (gizzard, heart, drumette and tulip) samples were collected from local supermarkets. As results, 47.36% of the samples were positives for E. coli. Furthermore, 10 pairs of clones of E. coli were found always in the same substrate (two waterwater pairs; three soil-soil pairs and five meat-meat pairs) using PFGE. These findings suggest that certain strains of E. coli may have habitat preferences, making the transfer from one substrate type to another more difficult to occur. Moreover, since no clones were found between environmental samples and chicken meat, it is possible to imply a low risk of E. coli transmissibility throughout the chicken meat production chain.application/pdfengRevista brasileira de ciência avícola = Brazilian journal of poultry science. Campinas, SP. Vol. 23, no. 1 (2021), eRBCA-2020-1361, 6 p.Escherichia coliCarne de frangoCama de frangosSoloÁguaEletroforese em gel de campo pulsadoMicrobiologyEnvironmentEpidemiologyPublic healthPulsed field gel electrophoresisGenetic similarities of Escherichia coli isolated from different substrates of the broiler production chaininfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001134010.pdf.txt001134010.pdf.txtExtracted Texttext/plain22699http://www.lume.ufrgs.br/bitstream/10183/232972/2/001134010.pdf.txt1d02789c0683e70906daae61ed9bb986MD52ORIGINAL001134010.pdfTexto completo (inglês)application/pdf1904564http://www.lume.ufrgs.br/bitstream/10183/232972/1/001134010.pdf611ee12ff57dde5f190046246363e361MD5110183/2329722022-01-07 05:28:58.688106oai:www.lume.ufrgs.br:10183/232972Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2022-01-07T07:28:58Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Genetic similarities of Escherichia coli isolated from different substrates of the broiler production chain |
title |
Genetic similarities of Escherichia coli isolated from different substrates of the broiler production chain |
spellingShingle |
Genetic similarities of Escherichia coli isolated from different substrates of the broiler production chain Teles, Pedro Filipe de Souza Escherichia coli Carne de frango Cama de frangos Solo Água Eletroforese em gel de campo pulsado Microbiology Environment Epidemiology Public health Pulsed field gel electrophoresis |
title_short |
Genetic similarities of Escherichia coli isolated from different substrates of the broiler production chain |
title_full |
Genetic similarities of Escherichia coli isolated from different substrates of the broiler production chain |
title_fullStr |
Genetic similarities of Escherichia coli isolated from different substrates of the broiler production chain |
title_full_unstemmed |
Genetic similarities of Escherichia coli isolated from different substrates of the broiler production chain |
title_sort |
Genetic similarities of Escherichia coli isolated from different substrates of the broiler production chain |
author |
Teles, Pedro Filipe de Souza |
author_facet |
Teles, Pedro Filipe de Souza Boiago, Marcel Manente Frigo, Angélica Rampazzo, Luana Araujo, Denise Nunes Kich, Jalusa Deon Rebelatto, Raquel Furian, Thales Quedi Stefani, Lenita de Cássia Moura |
author_role |
author |
author2 |
Boiago, Marcel Manente Frigo, Angélica Rampazzo, Luana Araujo, Denise Nunes Kich, Jalusa Deon Rebelatto, Raquel Furian, Thales Quedi Stefani, Lenita de Cássia Moura |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Teles, Pedro Filipe de Souza Boiago, Marcel Manente Frigo, Angélica Rampazzo, Luana Araujo, Denise Nunes Kich, Jalusa Deon Rebelatto, Raquel Furian, Thales Quedi Stefani, Lenita de Cássia Moura |
dc.subject.por.fl_str_mv |
Escherichia coli Carne de frango Cama de frangos Solo Água Eletroforese em gel de campo pulsado |
topic |
Escherichia coli Carne de frango Cama de frangos Solo Água Eletroforese em gel de campo pulsado Microbiology Environment Epidemiology Public health Pulsed field gel electrophoresis |
dc.subject.eng.fl_str_mv |
Microbiology Environment Epidemiology Public health Pulsed field gel electrophoresis |
description |
Brazil is the largest exporter of chicken meat and poultry farming is one of the most important productive segments, despite major losses due to the bacterium Escherichia coli, which is also a zoonotic microorganism. The objetive of this study was to isolate E. coli and to evaluate its transmissibility potential from the field to chicken meat using the Pulsed Field Gel Electrophoresis (PFGE) technique. Environmental samples (poultry litter, soil and water) were collected from broiler farms located in the South of Brazil where the majority of the Brazilian poultry production occurs. In addition, chicken meat (gizzard, heart, drumette and tulip) samples were collected from local supermarkets. As results, 47.36% of the samples were positives for E. coli. Furthermore, 10 pairs of clones of E. coli were found always in the same substrate (two waterwater pairs; three soil-soil pairs and five meat-meat pairs) using PFGE. These findings suggest that certain strains of E. coli may have habitat preferences, making the transfer from one substrate type to another more difficult to occur. Moreover, since no clones were found between environmental samples and chicken meat, it is possible to imply a low risk of E. coli transmissibility throughout the chicken meat production chain. |
publishDate |
2021 |
dc.date.accessioned.fl_str_mv |
2021-12-15T04:26:20Z |
dc.date.issued.fl_str_mv |
2021 |
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info:eu-repo/semantics/article info:eu-repo/semantics/other |
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http://hdl.handle.net/10183/232972 |
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1516-635X |
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001134010 |
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http://hdl.handle.net/10183/232972 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Revista brasileira de ciência avícola = Brazilian journal of poultry science. Campinas, SP. Vol. 23, no. 1 (2021), eRBCA-2020-1361, 6 p. |
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info:eu-repo/semantics/openAccess |
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openAccess |
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