Bioactivity and action mechanism of green propolis against Pythium aphanidermatum.

Detalhes bibliográficos
Autor(a) principal: PAZIN, W. M.
Data de Publicação: 2019
Outros Autores: SANTOS, S. N., QUEIROZ, S. C. do N. de, BAGATOLLI, L. A., SOARES, A. E. E., MELO, I. S. de, ITO, A. S.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1114957
http://dx.doi.org/10.1590/0001-3765201920180598
Resumo: Abstract: We have established how natural compounds from green propolis collected by the species Apis mellifera act against the growth of Pythium aphanidermatum. On the basis of mass spectrometry (Q-ToF MS), we determined that Artepillin C, the major constituent of green propolis, underlies the effect and displays activity against P. aphanidermatum at a minimal inhibitory concentration of 750 ?g.ml-1. Biophysical studies based on model membranes showed that this inhibitory effect may be linked with a membrane-related phenomenon: Artepillin C increases the permeability of membranes with relatively high fluidity in their lateral structure, a feature that is in line with the lipid composition reported for the cytoplasmic membrane of P. aphanidermatum. Therefore, the present study supports the use of the effective and inexpensive green propolis to control the impact of the dangerous phytopathogen P. aphanidermatumon agriculture.
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spelling Bioactivity and action mechanism of green propolis against Pythium aphanidermatum.Green propolisArtepillin CModel membranesPrópolisPythium aphanidermatumControle biológicoDoença de PlantaBiological controlPythiumPlant diseases and disordersAbstract: We have established how natural compounds from green propolis collected by the species Apis mellifera act against the growth of Pythium aphanidermatum. On the basis of mass spectrometry (Q-ToF MS), we determined that Artepillin C, the major constituent of green propolis, underlies the effect and displays activity against P. aphanidermatum at a minimal inhibitory concentration of 750 ?g.ml-1. Biophysical studies based on model membranes showed that this inhibitory effect may be linked with a membrane-related phenomenon: Artepillin C increases the permeability of membranes with relatively high fluidity in their lateral structure, a feature that is in line with the lipid composition reported for the cytoplasmic membrane of P. aphanidermatum. Therefore, the present study supports the use of the effective and inexpensive green propolis to control the impact of the dangerous phytopathogen P. aphanidermatumon agriculture.WALLANCE MOREIRA PAZIN, FFCLRP-USP; SUIKINAI NOBRE SANTOS; SONIA CLAUDIA DO N DE QUEIROZ, CNPMA; LUIS ALBERTO BAGATOLLI, Instituto de Investigaciones Medicas Mercedes y Martín Ferreyra/; ADEMILSON ESPENCER EGEA SOARES, FMRP-USP; ITAMAR SOARES DE MELO, CNPMA; AMANDO SIUITI ITO, FFCLRP-USP.PAZIN, W. M.SANTOS, S. N.QUEIROZ, S. C. do N. deBAGATOLLI, L. A.SOARES, A. E. E.MELO, I. S. deITO, A. S.2019-11-23T00:37:03Z2019-11-23T00:37:03Z2019-11-2220192019-11-23T00:37:03Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleAnais da Academia Brasileira de Ciências, v. 91, n. 2, 2019. Article e20180598.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1114957http://dx.doi.org/10.1590/0001-3765201920180598enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2019-11-23T00:37:10Zoai:www.alice.cnptia.embrapa.br:doc/1114957Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542019-11-23T00:37:10falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542019-11-23T00:37:10Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Bioactivity and action mechanism of green propolis against Pythium aphanidermatum.
title Bioactivity and action mechanism of green propolis against Pythium aphanidermatum.
spellingShingle Bioactivity and action mechanism of green propolis against Pythium aphanidermatum.
PAZIN, W. M.
Green propolis
Artepillin C
Model membranes
Própolis
Pythium aphanidermatum
Controle biológico
Doença de Planta
Biological control
Pythium
Plant diseases and disorders
title_short Bioactivity and action mechanism of green propolis against Pythium aphanidermatum.
title_full Bioactivity and action mechanism of green propolis against Pythium aphanidermatum.
title_fullStr Bioactivity and action mechanism of green propolis against Pythium aphanidermatum.
title_full_unstemmed Bioactivity and action mechanism of green propolis against Pythium aphanidermatum.
title_sort Bioactivity and action mechanism of green propolis against Pythium aphanidermatum.
author PAZIN, W. M.
author_facet PAZIN, W. M.
SANTOS, S. N.
QUEIROZ, S. C. do N. de
BAGATOLLI, L. A.
SOARES, A. E. E.
MELO, I. S. de
ITO, A. S.
author_role author
author2 SANTOS, S. N.
QUEIROZ, S. C. do N. de
BAGATOLLI, L. A.
SOARES, A. E. E.
MELO, I. S. de
ITO, A. S.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv WALLANCE MOREIRA PAZIN, FFCLRP-USP; SUIKINAI NOBRE SANTOS; SONIA CLAUDIA DO N DE QUEIROZ, CNPMA; LUIS ALBERTO BAGATOLLI, Instituto de Investigaciones Medicas Mercedes y Martín Ferreyra/; ADEMILSON ESPENCER EGEA SOARES, FMRP-USP; ITAMAR SOARES DE MELO, CNPMA; AMANDO SIUITI ITO, FFCLRP-USP.
dc.contributor.author.fl_str_mv PAZIN, W. M.
SANTOS, S. N.
QUEIROZ, S. C. do N. de
BAGATOLLI, L. A.
SOARES, A. E. E.
MELO, I. S. de
ITO, A. S.
dc.subject.por.fl_str_mv Green propolis
Artepillin C
Model membranes
Própolis
Pythium aphanidermatum
Controle biológico
Doença de Planta
Biological control
Pythium
Plant diseases and disorders
topic Green propolis
Artepillin C
Model membranes
Própolis
Pythium aphanidermatum
Controle biológico
Doença de Planta
Biological control
Pythium
Plant diseases and disorders
description Abstract: We have established how natural compounds from green propolis collected by the species Apis mellifera act against the growth of Pythium aphanidermatum. On the basis of mass spectrometry (Q-ToF MS), we determined that Artepillin C, the major constituent of green propolis, underlies the effect and displays activity against P. aphanidermatum at a minimal inhibitory concentration of 750 ?g.ml-1. Biophysical studies based on model membranes showed that this inhibitory effect may be linked with a membrane-related phenomenon: Artepillin C increases the permeability of membranes with relatively high fluidity in their lateral structure, a feature that is in line with the lipid composition reported for the cytoplasmic membrane of P. aphanidermatum. Therefore, the present study supports the use of the effective and inexpensive green propolis to control the impact of the dangerous phytopathogen P. aphanidermatumon agriculture.
publishDate 2019
dc.date.none.fl_str_mv 2019-11-23T00:37:03Z
2019-11-23T00:37:03Z
2019-11-22
2019
2019-11-23T00:37:03Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Anais da Academia Brasileira de Ciências, v. 91, n. 2, 2019. Article e20180598.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1114957
http://dx.doi.org/10.1590/0001-3765201920180598
identifier_str_mv Anais da Academia Brasileira de Ciências, v. 91, n. 2, 2019. Article e20180598.
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/1114957
http://dx.doi.org/10.1590/0001-3765201920180598
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
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