Bioactivity and action mechanism of green propolis against Pythium aphanidermatum

Detalhes bibliográficos
Autor(a) principal: Pazin, Wallance M. [UNESP]
Data de Publicação: 2019
Outros Autores: Dos Santos, Suikinai N., Queiroz, Sonia C. N., Bagatolli, Luis A., Soares, Ademilson E. E., De Melo, Itamar S., Ito, Amando S.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/0001-3765201920180598
http://hdl.handle.net/11449/184558
Resumo: We have established how natural compounds from green propolis collected by the species Apis mellifera act against the growth of Pythium aphanidermatum. On the basis of mass spectrometry (Q-ToF MS), we determined that Artepillin C, the major constituent of green propolis, underlies the effect and displays activity against P aphanidermatum at a minimal inhibitory concentration of 750 mu g.mL(-1). Biophysical studies based on model membranes showed that this inhibitory effect may be linked with a membrane-related phenomenon: Artepillin C increases the permeability of membranes with relatively high fluidity in their lateral structure, a feature that is in line with the lipid composition reported for the cytoplasmic membrane of P. aphanidermatum. Therefore, the present study supports the use of the effective and inexpensive green propolis to control the impact of the dangerous phytopathogen P. aphanidermatum on agriculture.
id UNSP_5de3f2cdbc65de8dc9659cddc61766d3
oai_identifier_str oai:repositorio.unesp.br:11449/184558
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Bioactivity and action mechanism of green propolis against Pythium aphanidermatumgreen propolisArtepillin Cfungicidemodel membranesPythium aphanidermatumWe have established how natural compounds from green propolis collected by the species Apis mellifera act against the growth of Pythium aphanidermatum. On the basis of mass spectrometry (Q-ToF MS), we determined that Artepillin C, the major constituent of green propolis, underlies the effect and displays activity against P aphanidermatum at a minimal inhibitory concentration of 750 mu g.mL(-1). Biophysical studies based on model membranes showed that this inhibitory effect may be linked with a membrane-related phenomenon: Artepillin C increases the permeability of membranes with relatively high fluidity in their lateral structure, a feature that is in line with the lipid composition reported for the cytoplasmic membrane of P. aphanidermatum. Therefore, the present study supports the use of the effective and inexpensive green propolis to control the impact of the dangerous phytopathogen P. aphanidermatum on agriculture.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Fis, Av Bandeirantes 3900, BR-14040901 Ribeirao Preto, SP, BrazilUniv Estadual Paulista, Fac Ciencias & Tecnol, Dept Fis, Rua Roberto Simonsen 305, BR-19060900 Presidente Prudente, SP, BrazilEmbrapa Meio Ambiente, Lab Microbiol Ambiental, Rodovia SP 340,Km 127,5, BR-13918110 Jaguariuna, SP, BrazilUNC, CONICET, INIMEC, Inst Invest Med Mercedes & Martin Ferreyra, RA-2434 Cordoba, ArgentinaUniv Sao Paulo, Fac Med Ribeirao Preto, Dept Genet, Av Bandeirantes 3900, BR-14049900 Ribeirao Preto, SP, BrazilUniv Estadual Paulista, Fac Ciencias & Tecnol, Dept Fis, Rua Roberto Simonsen 305, BR-19060900 Presidente Prudente, SP, BrazilCAPES: 004137/2014-00FAPESP: 2016/09633-4CNPq: 305771/2016-7Acad Brasileira De CienciasUniversidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)UNCPazin, Wallance M. [UNESP]Dos Santos, Suikinai N.Queiroz, Sonia C. N.Bagatolli, Luis A.Soares, Ademilson E. E.De Melo, Itamar S.Ito, Amando S.2019-10-04T12:14:36Z2019-10-04T12:14:36Z2019-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article9application/pdfhttp://dx.doi.org/10.1590/0001-3765201920180598Anais Da Academia Brasileira De Ciencias. Rio Janeiro: Acad Brasileira De Ciencias, v. 91, n. 2, 9 p., 2019.0001-3765http://hdl.handle.net/11449/18455810.1590/0001-3765201920180598S0001-37652019000300617WOS:000473789800001S0001-37652019000300617.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAnais Da Academia Brasileira De Cienciasinfo:eu-repo/semantics/openAccess2024-01-24T06:29:06Zoai:repositorio.unesp.br:11449/184558Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:49:12.825999Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Bioactivity and action mechanism of green propolis against Pythium aphanidermatum
title Bioactivity and action mechanism of green propolis against Pythium aphanidermatum
spellingShingle Bioactivity and action mechanism of green propolis against Pythium aphanidermatum
Pazin, Wallance M. [UNESP]
green propolis
Artepillin C
fungicide
model membranes
Pythium aphanidermatum
title_short Bioactivity and action mechanism of green propolis against Pythium aphanidermatum
title_full Bioactivity and action mechanism of green propolis against Pythium aphanidermatum
title_fullStr Bioactivity and action mechanism of green propolis against Pythium aphanidermatum
title_full_unstemmed Bioactivity and action mechanism of green propolis against Pythium aphanidermatum
title_sort Bioactivity and action mechanism of green propolis against Pythium aphanidermatum
author Pazin, Wallance M. [UNESP]
author_facet Pazin, Wallance M. [UNESP]
Dos Santos, Suikinai N.
Queiroz, Sonia C. N.
Bagatolli, Luis A.
Soares, Ademilson E. E.
De Melo, Itamar S.
Ito, Amando S.
author_role author
author2 Dos Santos, Suikinai N.
Queiroz, Sonia C. N.
Bagatolli, Luis A.
Soares, Ademilson E. E.
De Melo, Itamar S.
Ito, Amando S.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
Universidade Estadual Paulista (Unesp)
Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
UNC
dc.contributor.author.fl_str_mv Pazin, Wallance M. [UNESP]
Dos Santos, Suikinai N.
Queiroz, Sonia C. N.
Bagatolli, Luis A.
Soares, Ademilson E. E.
De Melo, Itamar S.
Ito, Amando S.
dc.subject.por.fl_str_mv green propolis
Artepillin C
fungicide
model membranes
Pythium aphanidermatum
topic green propolis
Artepillin C
fungicide
model membranes
Pythium aphanidermatum
description We have established how natural compounds from green propolis collected by the species Apis mellifera act against the growth of Pythium aphanidermatum. On the basis of mass spectrometry (Q-ToF MS), we determined that Artepillin C, the major constituent of green propolis, underlies the effect and displays activity against P aphanidermatum at a minimal inhibitory concentration of 750 mu g.mL(-1). Biophysical studies based on model membranes showed that this inhibitory effect may be linked with a membrane-related phenomenon: Artepillin C increases the permeability of membranes with relatively high fluidity in their lateral structure, a feature that is in line with the lipid composition reported for the cytoplasmic membrane of P. aphanidermatum. Therefore, the present study supports the use of the effective and inexpensive green propolis to control the impact of the dangerous phytopathogen P. aphanidermatum on agriculture.
publishDate 2019
dc.date.none.fl_str_mv 2019-10-04T12:14:36Z
2019-10-04T12:14:36Z
2019-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/0001-3765201920180598
Anais Da Academia Brasileira De Ciencias. Rio Janeiro: Acad Brasileira De Ciencias, v. 91, n. 2, 9 p., 2019.
0001-3765
http://hdl.handle.net/11449/184558
10.1590/0001-3765201920180598
S0001-37652019000300617
WOS:000473789800001
S0001-37652019000300617.pdf
url http://dx.doi.org/10.1590/0001-3765201920180598
http://hdl.handle.net/11449/184558
identifier_str_mv Anais Da Academia Brasileira De Ciencias. Rio Janeiro: Acad Brasileira De Ciencias, v. 91, n. 2, 9 p., 2019.
0001-3765
10.1590/0001-3765201920180598
S0001-37652019000300617
WOS:000473789800001
S0001-37652019000300617.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Anais Da Academia Brasileira De Ciencias
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 9
application/pdf
dc.publisher.none.fl_str_mv Acad Brasileira De Ciencias
publisher.none.fl_str_mv Acad Brasileira De Ciencias
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129555201785856