Bioactivity and action mechanism of green propolis against Pythium aphanidermatum
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/0001-3765201920180598 http://hdl.handle.net/11449/184558 |
Resumo: | We have established how natural compounds from green propolis collected by the species Apis mellifera act against the growth of Pythium aphanidermatum. On the basis of mass spectrometry (Q-ToF MS), we determined that Artepillin C, the major constituent of green propolis, underlies the effect and displays activity against P aphanidermatum at a minimal inhibitory concentration of 750 mu g.mL(-1). Biophysical studies based on model membranes showed that this inhibitory effect may be linked with a membrane-related phenomenon: Artepillin C increases the permeability of membranes with relatively high fluidity in their lateral structure, a feature that is in line with the lipid composition reported for the cytoplasmic membrane of P. aphanidermatum. Therefore, the present study supports the use of the effective and inexpensive green propolis to control the impact of the dangerous phytopathogen P. aphanidermatum on agriculture. |
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Bioactivity and action mechanism of green propolis against Pythium aphanidermatumgreen propolisArtepillin Cfungicidemodel membranesPythium aphanidermatumWe have established how natural compounds from green propolis collected by the species Apis mellifera act against the growth of Pythium aphanidermatum. On the basis of mass spectrometry (Q-ToF MS), we determined that Artepillin C, the major constituent of green propolis, underlies the effect and displays activity against P aphanidermatum at a minimal inhibitory concentration of 750 mu g.mL(-1). Biophysical studies based on model membranes showed that this inhibitory effect may be linked with a membrane-related phenomenon: Artepillin C increases the permeability of membranes with relatively high fluidity in their lateral structure, a feature that is in line with the lipid composition reported for the cytoplasmic membrane of P. aphanidermatum. Therefore, the present study supports the use of the effective and inexpensive green propolis to control the impact of the dangerous phytopathogen P. aphanidermatum on agriculture.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Fis, Av Bandeirantes 3900, BR-14040901 Ribeirao Preto, SP, BrazilUniv Estadual Paulista, Fac Ciencias & Tecnol, Dept Fis, Rua Roberto Simonsen 305, BR-19060900 Presidente Prudente, SP, BrazilEmbrapa Meio Ambiente, Lab Microbiol Ambiental, Rodovia SP 340,Km 127,5, BR-13918110 Jaguariuna, SP, BrazilUNC, CONICET, INIMEC, Inst Invest Med Mercedes & Martin Ferreyra, RA-2434 Cordoba, ArgentinaUniv Sao Paulo, Fac Med Ribeirao Preto, Dept Genet, Av Bandeirantes 3900, BR-14049900 Ribeirao Preto, SP, BrazilUniv Estadual Paulista, Fac Ciencias & Tecnol, Dept Fis, Rua Roberto Simonsen 305, BR-19060900 Presidente Prudente, SP, BrazilCAPES: 004137/2014-00FAPESP: 2016/09633-4CNPq: 305771/2016-7Acad Brasileira De CienciasUniversidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)UNCPazin, Wallance M. [UNESP]Dos Santos, Suikinai N.Queiroz, Sonia C. N.Bagatolli, Luis A.Soares, Ademilson E. E.De Melo, Itamar S.Ito, Amando S.2019-10-04T12:14:36Z2019-10-04T12:14:36Z2019-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article9application/pdfhttp://dx.doi.org/10.1590/0001-3765201920180598Anais Da Academia Brasileira De Ciencias. Rio Janeiro: Acad Brasileira De Ciencias, v. 91, n. 2, 9 p., 2019.0001-3765http://hdl.handle.net/11449/18455810.1590/0001-3765201920180598S0001-37652019000300617WOS:000473789800001S0001-37652019000300617.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAnais Da Academia Brasileira De Cienciasinfo:eu-repo/semantics/openAccess2024-01-24T06:29:06Zoai:repositorio.unesp.br:11449/184558Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:49:12.825999Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Bioactivity and action mechanism of green propolis against Pythium aphanidermatum |
title |
Bioactivity and action mechanism of green propolis against Pythium aphanidermatum |
spellingShingle |
Bioactivity and action mechanism of green propolis against Pythium aphanidermatum Pazin, Wallance M. [UNESP] green propolis Artepillin C fungicide model membranes Pythium aphanidermatum |
title_short |
Bioactivity and action mechanism of green propolis against Pythium aphanidermatum |
title_full |
Bioactivity and action mechanism of green propolis against Pythium aphanidermatum |
title_fullStr |
Bioactivity and action mechanism of green propolis against Pythium aphanidermatum |
title_full_unstemmed |
Bioactivity and action mechanism of green propolis against Pythium aphanidermatum |
title_sort |
Bioactivity and action mechanism of green propolis against Pythium aphanidermatum |
author |
Pazin, Wallance M. [UNESP] |
author_facet |
Pazin, Wallance M. [UNESP] Dos Santos, Suikinai N. Queiroz, Sonia C. N. Bagatolli, Luis A. Soares, Ademilson E. E. De Melo, Itamar S. Ito, Amando S. |
author_role |
author |
author2 |
Dos Santos, Suikinai N. Queiroz, Sonia C. N. Bagatolli, Luis A. Soares, Ademilson E. E. De Melo, Itamar S. Ito, Amando S. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) UNC |
dc.contributor.author.fl_str_mv |
Pazin, Wallance M. [UNESP] Dos Santos, Suikinai N. Queiroz, Sonia C. N. Bagatolli, Luis A. Soares, Ademilson E. E. De Melo, Itamar S. Ito, Amando S. |
dc.subject.por.fl_str_mv |
green propolis Artepillin C fungicide model membranes Pythium aphanidermatum |
topic |
green propolis Artepillin C fungicide model membranes Pythium aphanidermatum |
description |
We have established how natural compounds from green propolis collected by the species Apis mellifera act against the growth of Pythium aphanidermatum. On the basis of mass spectrometry (Q-ToF MS), we determined that Artepillin C, the major constituent of green propolis, underlies the effect and displays activity against P aphanidermatum at a minimal inhibitory concentration of 750 mu g.mL(-1). Biophysical studies based on model membranes showed that this inhibitory effect may be linked with a membrane-related phenomenon: Artepillin C increases the permeability of membranes with relatively high fluidity in their lateral structure, a feature that is in line with the lipid composition reported for the cytoplasmic membrane of P. aphanidermatum. Therefore, the present study supports the use of the effective and inexpensive green propolis to control the impact of the dangerous phytopathogen P. aphanidermatum on agriculture. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-04T12:14:36Z 2019-10-04T12:14:36Z 2019-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/0001-3765201920180598 Anais Da Academia Brasileira De Ciencias. Rio Janeiro: Acad Brasileira De Ciencias, v. 91, n. 2, 9 p., 2019. 0001-3765 http://hdl.handle.net/11449/184558 10.1590/0001-3765201920180598 S0001-37652019000300617 WOS:000473789800001 S0001-37652019000300617.pdf |
url |
http://dx.doi.org/10.1590/0001-3765201920180598 http://hdl.handle.net/11449/184558 |
identifier_str_mv |
Anais Da Academia Brasileira De Ciencias. Rio Janeiro: Acad Brasileira De Ciencias, v. 91, n. 2, 9 p., 2019. 0001-3765 10.1590/0001-3765201920180598 S0001-37652019000300617 WOS:000473789800001 S0001-37652019000300617.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Anais Da Academia Brasileira De Ciencias |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
9 application/pdf |
dc.publisher.none.fl_str_mv |
Acad Brasileira De Ciencias |
publisher.none.fl_str_mv |
Acad Brasileira De Ciencias |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129555201785856 |