Identification and characterization of pathogenic Pestalotiopsis species to pecan tree in Brazil.
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/994649 |
Resumo: | The objective of this work was to characterize and cluster isolates of Pestalotiopsis species and to identify those that are pathogenic to pecan, based on morphological and molecular characters. Pestalotiopsis spp. isolates were identified by sequencing the internal transcribed spacer (ITS) and β‑tubulin regions. Identification methods were compared to indicate the key morphological characters for species characterization. Thirteen isolates were used for the pathogenicity tests. Morphological characterization was performed using the following variables: mycelial growth rate, sporulation, colony pigmentation, and conidial length and width. Ten pathogenic isolates were identified, three as Pestalotiopsis clavispora and three as P. cocculi. The other isolates remained as an undefined species. The morphological characters were efficient for an initial separation of the isolates, which were grouped according to differences at species level, mainly colony diameter, which was identified as an important morphological describer. Beta‑tubulin gene sequencing was less informative than the ITS region sequencing for species identification. |
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Identification and characterization of pathogenic Pestalotiopsis species to pecan tree in Brazil.Caracter morfológicoNogueira pecãFitopatologiaFilogeniaPatógenoCarya illinoinensisPestalotiopsisThe objective of this work was to characterize and cluster isolates of Pestalotiopsis species and to identify those that are pathogenic to pecan, based on morphological and molecular characters. Pestalotiopsis spp. isolates were identified by sequencing the internal transcribed spacer (ITS) and β‑tubulin regions. Identification methods were compared to indicate the key morphological characters for species characterization. Thirteen isolates were used for the pathogenicity tests. Morphological characterization was performed using the following variables: mycelial growth rate, sporulation, colony pigmentation, and conidial length and width. Ten pathogenic isolates were identified, three as Pestalotiopsis clavispora and three as P. cocculi. The other isolates remained as an undefined species. The morphological characters were efficient for an initial separation of the isolates, which were grouped according to differences at species level, mainly colony diameter, which was identified as an important morphological describer. Beta‑tubulin gene sequencing was less informative than the ITS region sequencing for species identification.MARÍLIA LAZAROTTO, UFRGS; MARCIÉLI PITORINI BOVOLINI, Universidade Federal de Santa Maria; MARLOVE FÁTIMA BRIÃO MUNIZ, Universidade Federal de Santa Maria; RICARDO HARAKAWA, Instituto Biológico de São Paulo; LIA REJANE SILVEIRA REINIGER, Universidade Federal de Santa Maria; ALVARO FIGUEREDO DOS SANTOS, CNPF.LAZAROTTO, M.BOVOLINI, M. P.MUNIZ, M. F. B.HARAKAWA, R.REINIGER, L. R. S.SANTOS, A. F. dos2014-09-11T06:51:27Z2014-09-11T06:51:27Z2014-09-1020142014-09-11T06:51:27Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePesquisa Agropecuária Brasileira, Brasília, DF, v. 49, n. 6, p. 440-448, jun. 2014.http://www.alice.cnptia.embrapa.br/alice/handle/doc/994649enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-16T02:01:35Zoai:www.alice.cnptia.embrapa.br:doc/994649Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-16T02:01:35falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-16T02:01:35Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Identification and characterization of pathogenic Pestalotiopsis species to pecan tree in Brazil. |
title |
Identification and characterization of pathogenic Pestalotiopsis species to pecan tree in Brazil. |
spellingShingle |
Identification and characterization of pathogenic Pestalotiopsis species to pecan tree in Brazil. LAZAROTTO, M. Caracter morfológico Nogueira pecã Fitopatologia Filogenia Patógeno Carya illinoinensis Pestalotiopsis |
title_short |
Identification and characterization of pathogenic Pestalotiopsis species to pecan tree in Brazil. |
title_full |
Identification and characterization of pathogenic Pestalotiopsis species to pecan tree in Brazil. |
title_fullStr |
Identification and characterization of pathogenic Pestalotiopsis species to pecan tree in Brazil. |
title_full_unstemmed |
Identification and characterization of pathogenic Pestalotiopsis species to pecan tree in Brazil. |
title_sort |
Identification and characterization of pathogenic Pestalotiopsis species to pecan tree in Brazil. |
author |
LAZAROTTO, M. |
author_facet |
LAZAROTTO, M. BOVOLINI, M. P. MUNIZ, M. F. B. HARAKAWA, R. REINIGER, L. R. S. SANTOS, A. F. dos |
author_role |
author |
author2 |
BOVOLINI, M. P. MUNIZ, M. F. B. HARAKAWA, R. REINIGER, L. R. S. SANTOS, A. F. dos |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
MARÍLIA LAZAROTTO, UFRGS; MARCIÉLI PITORINI BOVOLINI, Universidade Federal de Santa Maria; MARLOVE FÁTIMA BRIÃO MUNIZ, Universidade Federal de Santa Maria; RICARDO HARAKAWA, Instituto Biológico de São Paulo; LIA REJANE SILVEIRA REINIGER, Universidade Federal de Santa Maria; ALVARO FIGUEREDO DOS SANTOS, CNPF. |
dc.contributor.author.fl_str_mv |
LAZAROTTO, M. BOVOLINI, M. P. MUNIZ, M. F. B. HARAKAWA, R. REINIGER, L. R. S. SANTOS, A. F. dos |
dc.subject.por.fl_str_mv |
Caracter morfológico Nogueira pecã Fitopatologia Filogenia Patógeno Carya illinoinensis Pestalotiopsis |
topic |
Caracter morfológico Nogueira pecã Fitopatologia Filogenia Patógeno Carya illinoinensis Pestalotiopsis |
description |
The objective of this work was to characterize and cluster isolates of Pestalotiopsis species and to identify those that are pathogenic to pecan, based on morphological and molecular characters. Pestalotiopsis spp. isolates were identified by sequencing the internal transcribed spacer (ITS) and β‑tubulin regions. Identification methods were compared to indicate the key morphological characters for species characterization. Thirteen isolates were used for the pathogenicity tests. Morphological characterization was performed using the following variables: mycelial growth rate, sporulation, colony pigmentation, and conidial length and width. Ten pathogenic isolates were identified, three as Pestalotiopsis clavispora and three as P. cocculi. The other isolates remained as an undefined species. The morphological characters were efficient for an initial separation of the isolates, which were grouped according to differences at species level, mainly colony diameter, which was identified as an important morphological describer. Beta‑tubulin gene sequencing was less informative than the ITS region sequencing for species identification. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-09-11T06:51:27Z 2014-09-11T06:51:27Z 2014-09-10 2014 2014-09-11T06:51:27Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Pesquisa Agropecuária Brasileira, Brasília, DF, v. 49, n. 6, p. 440-448, jun. 2014. http://www.alice.cnptia.embrapa.br/alice/handle/doc/994649 |
identifier_str_mv |
Pesquisa Agropecuária Brasileira, Brasília, DF, v. 49, n. 6, p. 440-448, jun. 2014. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/994649 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503394059091968 |