Effect of Monocerin, a fungal secondary metabolite, on endothelial cells.

• Autor(a) principal: GOMES, T. C.
• Data de Publicação: 2023
• Outros Autores: CONRADO, R., OLIVEIRA, R. C. de, SELARI, P. J. R. G., MELO, I. S. de, ARAÚJO, W. L., MARIA, D. A., SOUZA, A. O. de
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
• Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1154958; https://doi.org/10.3390/toxins15050344
• topic: Monocerin; Cróton; Caatinga; Fungo; Endophytes; Fungi; Exserohilum; Medicinal fungi; Secondary metabolites; Cell viability; Cell senescence

Abstract: This study reports the isolation and identification of the endophytic fungus Exserohilum rostratum through molecular and morphological analysis using optical and transmission electron microscopy (TEM), as well as the procurement of its secondary metabolite monocerin, an isocoumarin derivative. Considering the previously observed biological activities of monocerin, this study was performed on human umbilical vein endothelial cells (HUVECs) that are widely used as an in vitro model for several different purposes. Important parameters, such as cell viability, senescence-associated ?-galactosidase, cellular proliferation by using 5(6)-carboxyfluorescein diacetate N-succinimidyl ester (CFSE), apoptosis analysis with annexin, cellular morphology through scanning electron microscopy (SEM), and laser confocal analysis were evaluated after exposing the cells to monocerin. After 24 h of exposure to monocerin at 1.25 mM, there was more than 80% of cell viability and a low percentage of cells in the early and late apoptosis and necrosis. Monocerin increased cell proliferation and did not induce cell senescence. Morphological analysis showed cellular integrity. The study demonstrates aspects of the mechanism of action of monocerin on endothelial cell proliferation, suggesting the possibility of its pharmaceutical application, such as in regenerative medicine.