Assessment of morphophysiological and genotypic diversity of endophytic bacteria isolated from rice (Oryza sativa L.) plants.
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1159886 |
Resumo: | Rice production in Brazil incurs high costs due to the significant use of agrochemicals. Some plant growth-promoting rhizobacteria (PGPR) can be used as alternative to fertilizers and phytosanitary products. Thus, the objective of this study was to characterize endophytic bacteria isolated from roots of rice plants. The isolates were characterized based on colony morphology, antibiotic resistance, carbon sources utilization, enzyme activity (catalase, amylase, protease, cellulase, and lipase), inorganic phosphate solubilization, and the 16S-23S rDNA intergenic region. Morphologically, 68% of the isolates presented a rapid growth rate, 46% presented abundant mucus production, and 77% formed viscous colonies. All isolates were resistant to nalidixic acid and 16% presented resistance to streptomycin. The majority (90%) used monosaccharides and disaccharides in carbon source assays. Most of the isolates (95%) were positive for catalase and 63.6% were positive for amylase, protease, lipase, and cellulase activities. Additionally, 59% of them were able to solubilize phosphate. The mean enzymatic index for amylase, cellulase, and protease was 2.8, 3.5, and 1.7 respectively. The similarity analysis revealed high diversity among the isolates, with similarity indices of 70% (based on morphological characteristics) and 60% (based on the intergenic region 16S-23S rDNA). Considering morphophysiological and genotypic characteristics, three promising isolates should be evaluated in studies under field conditions for the potential development of bioproducts to replace industrially manufactured inputs in rice crops. |
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Assessment of morphophysiological and genotypic diversity of endophytic bacteria isolated from rice (Oryza sativa L.) plants.Extracellular enzymesOCR 16S-23SRDNAArrozOryza SativaEnzimaFator de CrescimentoBactériaRiceGenotypePlant growth-promoting rhizobacteriaRice production in Brazil incurs high costs due to the significant use of agrochemicals. Some plant growth-promoting rhizobacteria (PGPR) can be used as alternative to fertilizers and phytosanitary products. Thus, the objective of this study was to characterize endophytic bacteria isolated from roots of rice plants. The isolates were characterized based on colony morphology, antibiotic resistance, carbon sources utilization, enzyme activity (catalase, amylase, protease, cellulase, and lipase), inorganic phosphate solubilization, and the 16S-23S rDNA intergenic region. Morphologically, 68% of the isolates presented a rapid growth rate, 46% presented abundant mucus production, and 77% formed viscous colonies. All isolates were resistant to nalidixic acid and 16% presented resistance to streptomycin. The majority (90%) used monosaccharides and disaccharides in carbon source assays. Most of the isolates (95%) were positive for catalase and 63.6% were positive for amylase, protease, lipase, and cellulase activities. Additionally, 59% of them were able to solubilize phosphate. The mean enzymatic index for amylase, cellulase, and protease was 2.8, 3.5, and 1.7 respectively. The similarity analysis revealed high diversity among the isolates, with similarity indices of 70% (based on morphological characteristics) and 60% (based on the intergenic region 16S-23S rDNA). Considering morphophysiological and genotypic characteristics, three promising isolates should be evaluated in studies under field conditions for the potential development of bioproducts to replace industrially manufactured inputs in rice crops.LUCAS LEONARDO-SILVA, UEG; LARISSA B. DA SILVA, UEG; ENDERSON PETRONIO DE BRITO FERREIRA, CNPAF; KARINA F. D. N. S. DOS REIS, UEG; PLINIO L. F. NAVES, UEG; CLAUDIA C. G. MARTIN-DIDONET, UEG.LEONARDO-SILVA, L.SILVA, L. B. daFERREIRA, E. P. de B.REIS, K. F. D. N. S. dosNAVES, P. L. F.MARTIN-DIDONET, C. C. G.2023-12-18T16:32:34Z2023-12-18T16:32:34Z2023-12-182023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleRevista Caatinga, v. 36, n. 4, p. 775-784, out./dez. 2023.1983-2125http://www.alice.cnptia.embrapa.br/alice/handle/doc/1159886porinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2023-12-18T16:32:34Zoai:www.alice.cnptia.embrapa.br:doc/1159886Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542023-12-18T16:32:34falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542023-12-18T16:32:34Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Assessment of morphophysiological and genotypic diversity of endophytic bacteria isolated from rice (Oryza sativa L.) plants. |
title |
Assessment of morphophysiological and genotypic diversity of endophytic bacteria isolated from rice (Oryza sativa L.) plants. |
spellingShingle |
Assessment of morphophysiological and genotypic diversity of endophytic bacteria isolated from rice (Oryza sativa L.) plants. LEONARDO-SILVA, L. Extracellular enzymes OCR 16S-23S RDNA Arroz Oryza Sativa Enzima Fator de Crescimento Bactéria Rice Genotype Plant growth-promoting rhizobacteria |
title_short |
Assessment of morphophysiological and genotypic diversity of endophytic bacteria isolated from rice (Oryza sativa L.) plants. |
title_full |
Assessment of morphophysiological and genotypic diversity of endophytic bacteria isolated from rice (Oryza sativa L.) plants. |
title_fullStr |
Assessment of morphophysiological and genotypic diversity of endophytic bacteria isolated from rice (Oryza sativa L.) plants. |
title_full_unstemmed |
Assessment of morphophysiological and genotypic diversity of endophytic bacteria isolated from rice (Oryza sativa L.) plants. |
title_sort |
Assessment of morphophysiological and genotypic diversity of endophytic bacteria isolated from rice (Oryza sativa L.) plants. |
author |
LEONARDO-SILVA, L. |
author_facet |
LEONARDO-SILVA, L. SILVA, L. B. da FERREIRA, E. P. de B. REIS, K. F. D. N. S. dos NAVES, P. L. F. MARTIN-DIDONET, C. C. G. |
author_role |
author |
author2 |
SILVA, L. B. da FERREIRA, E. P. de B. REIS, K. F. D. N. S. dos NAVES, P. L. F. MARTIN-DIDONET, C. C. G. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
LUCAS LEONARDO-SILVA, UEG; LARISSA B. DA SILVA, UEG; ENDERSON PETRONIO DE BRITO FERREIRA, CNPAF; KARINA F. D. N. S. DOS REIS, UEG; PLINIO L. F. NAVES, UEG; CLAUDIA C. G. MARTIN-DIDONET, UEG. |
dc.contributor.author.fl_str_mv |
LEONARDO-SILVA, L. SILVA, L. B. da FERREIRA, E. P. de B. REIS, K. F. D. N. S. dos NAVES, P. L. F. MARTIN-DIDONET, C. C. G. |
dc.subject.por.fl_str_mv |
Extracellular enzymes OCR 16S-23S RDNA Arroz Oryza Sativa Enzima Fator de Crescimento Bactéria Rice Genotype Plant growth-promoting rhizobacteria |
topic |
Extracellular enzymes OCR 16S-23S RDNA Arroz Oryza Sativa Enzima Fator de Crescimento Bactéria Rice Genotype Plant growth-promoting rhizobacteria |
description |
Rice production in Brazil incurs high costs due to the significant use of agrochemicals. Some plant growth-promoting rhizobacteria (PGPR) can be used as alternative to fertilizers and phytosanitary products. Thus, the objective of this study was to characterize endophytic bacteria isolated from roots of rice plants. The isolates were characterized based on colony morphology, antibiotic resistance, carbon sources utilization, enzyme activity (catalase, amylase, protease, cellulase, and lipase), inorganic phosphate solubilization, and the 16S-23S rDNA intergenic region. Morphologically, 68% of the isolates presented a rapid growth rate, 46% presented abundant mucus production, and 77% formed viscous colonies. All isolates were resistant to nalidixic acid and 16% presented resistance to streptomycin. The majority (90%) used monosaccharides and disaccharides in carbon source assays. Most of the isolates (95%) were positive for catalase and 63.6% were positive for amylase, protease, lipase, and cellulase activities. Additionally, 59% of them were able to solubilize phosphate. The mean enzymatic index for amylase, cellulase, and protease was 2.8, 3.5, and 1.7 respectively. The similarity analysis revealed high diversity among the isolates, with similarity indices of 70% (based on morphological characteristics) and 60% (based on the intergenic region 16S-23S rDNA). Considering morphophysiological and genotypic characteristics, three promising isolates should be evaluated in studies under field conditions for the potential development of bioproducts to replace industrially manufactured inputs in rice crops. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-12-18T16:32:34Z 2023-12-18T16:32:34Z 2023-12-18 2023 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Revista Caatinga, v. 36, n. 4, p. 775-784, out./dez. 2023. 1983-2125 http://www.alice.cnptia.embrapa.br/alice/handle/doc/1159886 |
identifier_str_mv |
Revista Caatinga, v. 36, n. 4, p. 775-784, out./dez. 2023. 1983-2125 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1159886 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
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Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
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EMBRAPA |
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EMBRAPA |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
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cg-riaa@embrapa.br |
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1794503553979514880 |