Genome-wide analysis of the transcriptional response to drought stress in root and leaf of common bean.

• Autor(a) principal: PEREIRA, W. J.
• Data de Publicação: 2020
• Outros Autores: MELO, A. T. de O., COELHO, A. S. G., RODRIGUES, F. A., MAMIDI, S., ALENCAR, S. A. de, LANNA, A. C., VALDISSER, P. A. M. R., BRONDANI, C., NASCIMENTO JUNIOR, I. R. do, BORBA, T. C. de O., VIANELLO, R. P.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
• Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1121822; https://doi.org/10.1590/1678-4685-gmb-2018-0259
• topic: Functional genomics; Feijão; Phaseolus Vulgaris; Deficiência Hídrica; Raiz; Folha; Beans; Soil water deficit; Genomics; Single nucleotide polymorphism

Genes related to the response to drought stress in leaf and root tissue of drought-susceptible (DS) and tolerant (DT) genotypes were characterized by RNA-Seq. In total, 54,750 transcripts, representative of 28,590 genes, were identified; of these, 1,648 were of high-fidelity (merge of 12 libraries) and described for the first time in the Andean germplasm. From the 1,239 differentially expressed genes (DEGs), 458 were identified in DT, with a predominance of genes in categories of oxidative stress, response to stimulus and kinase activity. Most genes related to oxidation-reduction terms in roots were early triggered in DT (T75) compared to DS (T150) suggestive of a mechanism of tolerance by reducing the damage from ROS. Among the KEGG enriched by DEGs up-regulated in DT leaves, two related to the formation of Sulfur-containing compounds, which are known for their involvement in tolerance to abiotic stresses, were common to all treatments. Through qPCR, 88.64% of the DEGs were validated. A total of 151,283 variants were identified and functional effects estimated for 85,780. The raw data files were submitted to the NCBI database. A transcriptome map revealed new genes and isoforms under drought. These results supports a better understanding of the drought tolerance mechanisms in beans.