Genome-wide analysis of the transcriptional response to drought stress in root and leaf of common bean

• Autor(a) principal: Pereira,Wendell Jacinto
• Data de Publicação: 2020
• Outros Autores: Melo,Arthur Tavares de Oliveira, Coelho,Alexandre Siqueira Guedes, Rodrigues,Fabiana Aparecida, Mamidi,Sujan, Alencar,Sérgio Amorim de, Lanna,Anna Cristina, Valdisser,Paula Arielle Mendes Ribeiro, Brondani,Claudio, Nascimento-Júnior,Ivanildo Ramalho do, Borba,Tereza Cristina de Oliveira, Vianello,Rosana Pereira
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Genetics and Molecular Biology
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100309
• topic: Phaseolus vulgaris; water deficit; functional genomics; single nucleotide polymorphism (SNP)

Abstract Genes related to the response to drought stress in leaf and root tissue of drought-susceptible (DS) and tolerant (DT) genotypes were characterized by RNA-Seq. In total, 54,750 transcripts, representative of 28,590 genes, were identified; of these, 1,648 were of high-fidelity (merge of 12 libraries) and described for the first time in the Andean germplasm. From the 1,239 differentially expressed genes (DEGs), 458 were identified in DT, with a predominance of genes in categories of oxidative stress, response to stimulus and kinase activity. Most genes related to oxidation-reduction terms in roots were early triggered in DT (T75) compared to DS (T150) suggestive of a mechanism of tolerance by reducing the damage from ROS. Among the KEGG enriched by DEGs up-regulated in DT leaves, two related to the formation of Sulfur-containing compounds, which are known for their involvement in tolerance to abiotic stresses, were common to all treatments. Through qPCR, 88.64% of the DEGs were validated. A total of 151,283 variants were identified and functional effects estimated for 85,780. The raw data files were submitted to the NCBI database. A transcriptome map revealed new genes and isoforms under drought. These results supports a better understanding of the drought tolerance mechanisms in beans.