[PROVISIONAL] Genome-wide analysis of the transcriptional response to drought stress in root and leaf of common bean

• Autor(a) principal: Pereira, Wendell Jacinto
• Data de Publicação: 2019
• Outros Autores: Melo, , Arthur Tavares De Oliveira, Coelho, Alexandre Siqueira Guedes, Rodrigues, Fabiana Aparecida, Mamidi, Sujan, Alencar, Sérgio Amorim De, Lanna, Anna Cristina, Valdisser, Paula Arielle Mendes Ribeiro, Brondani, Claudio, Nascimento-júnior, Ivanildo Ramalho Do, Borba, Tereza Cristina De Oliveira, Vianello, Rosana Pereira
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.1590/1678-4685-gmb-2018-0259; http://hdl.handle.net/11449/183765
• topic: Phaseolus vulgaris; water deficit; functional genomics; Single Nucleotide Polymorphism (SNP)

Abstract Genes related to the response to drought stress in leaf and root tissue of drought-susceptible (DS) and tolerant (DT) genotypes were characterized by RNA-Seq. In total, 54,750 transcripts, representative of 28,590 genes, were identified; of these, 1,648 were of high-fidelity (merge of 12 libraries) and described for the first time in the Andean germplasm. From the 1,239 differentially expressed genes (DEGs), 458 were identified in DT, with a predominance of genes in categories of oxidative stress, response to stimulus and kinase activity. Most genes related to oxidation-reduction terms in roots were early triggered in DT (T75) compared to DS (T150) suggestive of a mechanism of tolerance by reducing the damage from ROS. Among the KEGG enriched by DEGs up-regulated in DT leaves, two related to the formation of Sulfur-containing compounds, which are known for their involvement in tolerance to abiotic stresses, were common to all treatments. Through qPCR, 88.64% of the DEGs were validated. A total of 151,283 variants were identified and functional effects estimated for 85,780. The raw data files were submitted to the NCBI database. A transcriptome map revealed new genes and isoforms under drought. These results supports a better understanding of the drought tolerance mechanisms in beans.