Determination of moxidectin in serum by liquid chromatography-tandem mass spectrometry and its application in pharmacokinetic study in lambs.

• Autor(a) principal: BAPTISTA, R. C.
• Data de Publicação: 2017
• Outros Autores: FERNANDES, M. A. M., GILAVERTE, S., QUEIROZ, S. C. do N. de, ASSALIN, M. R., FERRACINI, V. L., MONTEIRO, A. L. G., REYES, F. G. R.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
• Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1060930
• topic: Serum lamb; LC-MS/MS; Cordeiro; Análise de laboratório; Anti-helmíntico; Cromatografia; Espectrometria; Soro Sangüíneo; Blood serum; Lambs; Moxidectin; Anthelmintics; Pharmacokinetics; Veterinary drugs; Tandem mass spectrometry; Liquid chromatography

Abstract: The widespread use of moxidectin (MOX), a parasiticide used in the sheep breeding, has induced the parasite resistance in Brazilian farms. As a consequence, the farmers often increase the dose and frequency of drug utilization, and disregards safety of meat or milk. In order to establish adequate therapeutic treatment it is necessary to know the pharmacokinetics of the drug in the animal's body. Thus, high performance liquid chromatography-electrospray ionization-tandem mass spectrometry (HPLC-ESI-MS/MS) method was developed for the determination of MOX in serum lamb. Serum samples were treated with acetonitrile to precipitate proteins. A clean up by dispersive extraction in solid phase (SPE-d), using primary/secondary amine (PSA) and C18 sorbents, followed by freezing was performed. Method validation presented precision (coefficient of variation) and accuracy (recovery%) between 1.7-6.7 and 80.0-107.3%, respectively. The limit of quantification (LOQ) of the method was 2.0 ng mL-1 and a linear response was obtained over a range of 2.0 to 100 ng mL-1. This method was successfully applied to the determination of MOX in serum from suffolk lamb to evaluate the pharmacokinetic profile.