Effect of freezing and storage time of blood serum of lambs in the determination of biochemical parameters
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Semina. Ciências Agrárias (Online) |
Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/4795 |
Resumo: | This study aimed to evaluate the effect of freezing (- 20 ºC) and time of storage of serum in the determination of biochemical values in sheep. It was held at the Universidade Estadual do Centro-Oeste in Guarapuava – PR. The serum was obtained from 32 Corriedale x Ille de France crossbred lambs, four months old. Nine hundred and sixty tests were performed, the first immediately after sampling (time zero), after 7, 14, 21, 28 days after blood collection. The analytes tested were aspartate aminotranferase (AST), urea, total protein, cholesterol, phosphorus and magnesium. The results showed that the time of storage of frozen samples (-20 ºC) was able to alter results of total protein, phosphorus, and magnesium. The serum measurements of AST, cholesterol and urea did not differ in different storage times. |
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Semina. Ciências Agrárias (Online) |
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Effect of freezing and storage time of blood serum of lambs in the determination of biochemical parametersEfeito do congelamento e do tempo de armazenamento do soro sanguíneo de cordeiros na determinação de parâmetros bioquímicosSerum stabilitySheepsTime of storage.Estabilidade do soroCordeirosTempo de armazenamento.This study aimed to evaluate the effect of freezing (- 20 ºC) and time of storage of serum in the determination of biochemical values in sheep. It was held at the Universidade Estadual do Centro-Oeste in Guarapuava – PR. The serum was obtained from 32 Corriedale x Ille de France crossbred lambs, four months old. Nine hundred and sixty tests were performed, the first immediately after sampling (time zero), after 7, 14, 21, 28 days after blood collection. The analytes tested were aspartate aminotranferase (AST), urea, total protein, cholesterol, phosphorus and magnesium. The results showed that the time of storage of frozen samples (-20 ºC) was able to alter results of total protein, phosphorus, and magnesium. The serum measurements of AST, cholesterol and urea did not differ in different storage times.Este trabalho teve como objetivo avaliar o efeito do congelamento (- 20 ºC) e o tempo de armazenamento do soro na determinação de valores bioquímicos em ovinos. Foi realizado na Universidade Estadual do Centro-Oeste em Guarapuava – PR. O soro foi obtido de 32 cordeiros da raça Ile de France x Corriedale, com 4 meses de idade. Foram realizadas análises, imediatamente após a obtenção da amostra, tempo zero e após 7, 14, 21, 28 dias da colheita do sangue. Os analitos testados foram aspartato-amino-transferase (AST), uréia, proteína total, colesterol, fósforo e magnésio. Os resultados permitiram concluir que o tempo de armazenamento das amostras congeladas (- 20 ºC) causou alterações nos resultados para as análises de proteína total, fósforo, e magnésio. As determinações séricas AST, colesterol e uréia não diferiram estatisticamente nos diferentes tempos de armazenamento. UEL2011-07-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionpesquisa empírica de campoapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/479510.5433/1679-0359.2011v32n2p717Semina: Ciências Agrárias; Vol. 32 No. 2 (2011); 717-722Semina: Ciências Agrárias; v. 32 n. 2 (2011); 717-7221679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELporhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/4795/8430Oliveira, Fernanda Schoma deFalbo, Margarete KimieSandini, Itacir EloiIshiy, Larissa Emyinfo:eu-repo/semantics/openAccess2016-03-09T17:00:22Zoai:ojs.pkp.sfu.ca:article/4795Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2016-03-09T17:00:22Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
dc.title.none.fl_str_mv |
Effect of freezing and storage time of blood serum of lambs in the determination of biochemical parameters Efeito do congelamento e do tempo de armazenamento do soro sanguíneo de cordeiros na determinação de parâmetros bioquímicos |
title |
Effect of freezing and storage time of blood serum of lambs in the determination of biochemical parameters |
spellingShingle |
Effect of freezing and storage time of blood serum of lambs in the determination of biochemical parameters Oliveira, Fernanda Schoma de Serum stability Sheeps Time of storage. Estabilidade do soro Cordeiros Tempo de armazenamento. |
title_short |
Effect of freezing and storage time of blood serum of lambs in the determination of biochemical parameters |
title_full |
Effect of freezing and storage time of blood serum of lambs in the determination of biochemical parameters |
title_fullStr |
Effect of freezing and storage time of blood serum of lambs in the determination of biochemical parameters |
title_full_unstemmed |
Effect of freezing and storage time of blood serum of lambs in the determination of biochemical parameters |
title_sort |
Effect of freezing and storage time of blood serum of lambs in the determination of biochemical parameters |
author |
Oliveira, Fernanda Schoma de |
author_facet |
Oliveira, Fernanda Schoma de Falbo, Margarete Kimie Sandini, Itacir Eloi Ishiy, Larissa Emy |
author_role |
author |
author2 |
Falbo, Margarete Kimie Sandini, Itacir Eloi Ishiy, Larissa Emy |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Oliveira, Fernanda Schoma de Falbo, Margarete Kimie Sandini, Itacir Eloi Ishiy, Larissa Emy |
dc.subject.por.fl_str_mv |
Serum stability Sheeps Time of storage. Estabilidade do soro Cordeiros Tempo de armazenamento. |
topic |
Serum stability Sheeps Time of storage. Estabilidade do soro Cordeiros Tempo de armazenamento. |
description |
This study aimed to evaluate the effect of freezing (- 20 ºC) and time of storage of serum in the determination of biochemical values in sheep. It was held at the Universidade Estadual do Centro-Oeste in Guarapuava – PR. The serum was obtained from 32 Corriedale x Ille de France crossbred lambs, four months old. Nine hundred and sixty tests were performed, the first immediately after sampling (time zero), after 7, 14, 21, 28 days after blood collection. The analytes tested were aspartate aminotranferase (AST), urea, total protein, cholesterol, phosphorus and magnesium. The results showed that the time of storage of frozen samples (-20 ºC) was able to alter results of total protein, phosphorus, and magnesium. The serum measurements of AST, cholesterol and urea did not differ in different storage times. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-07-12 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion pesquisa empírica de campo |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/4795 10.5433/1679-0359.2011v32n2p717 |
url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/4795 |
identifier_str_mv |
10.5433/1679-0359.2011v32n2p717 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/4795/8430 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UEL |
publisher.none.fl_str_mv |
UEL |
dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 32 No. 2 (2011); 717-722 Semina: Ciências Agrárias; v. 32 n. 2 (2011); 717-722 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
instname_str |
Universidade Estadual de Londrina (UEL) |
instacron_str |
UEL |
institution |
UEL |
reponame_str |
Semina. Ciências Agrárias (Online) |
collection |
Semina. Ciências Agrárias (Online) |
repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
repository.mail.fl_str_mv |
semina.agrarias@uel.br |
_version_ |
1799306061209927680 |