Antimicrobial and Photoantimicrobial Activities of Chitosan/CNPPV Nanocomposites.
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1148795 https://doi.org/10.3390/ijms232012519 |
Resumo: | Multidrug-resistant bacteria represent a global health and economic burden that urgently calls for new technologies to combat bacterial antimicrobial resistance. Here, we developed novel nanocomposites (NCPs) based on chitosan that display different degrees of acetylation (DAs), and conjugated polymer cyano-substituted poly(p-phenylene vinylene) (CNPPV) as an alternative approach to inactivate Gram-negative (E. coli) and Gram-positive (S. aureus) bacteria. Chitosan?s structure was confirmed through FT-Raman spectroscopy. Bactericidal and photobactericidal activities of NCPs were tested under dark and blue-light irradiation conditions, respectively. Hydrodynamic size and aqueous stability were determined by DLS, zeta potential (ZP) and time-domain NMR. TEM micrographs of NCPs were obtained, and their capacity of generating reactive oxygen species(ROS) under blue illumination was also characterized. Meaningful variations on ZP and relaxationtime T2 confirmed successful physical attachment of chitosan/CNPPV. All NCPs exhibited a similarand shrunken spherical shape according to TEM. A lower DA is responsible for driving higher bactericidal performance alongside the synergistic effect from CNPPV, lower nanosized distribution profile and higher positive charged surface. ROS production was proportionally found in NCPs with and without CNPPV by decreasing the DA, leading to a remarkable photobactericidal effect underblue-light irradiation. Overall, our findings indicate that chitosan/CNPPV NCPs may constitute a valuable asset for the development of innovative strategies for inactivation and/or photoinactivation of bacteria |
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Antimicrobial and Photoantimicrobial Activities of Chitosan/CNPPV Nanocomposites.Photoantimicrobial activityBlue-light irradiationCNPPVS aureusMultidrug-resistant bacteria represent a global health and economic burden that urgently calls for new technologies to combat bacterial antimicrobial resistance. Here, we developed novel nanocomposites (NCPs) based on chitosan that display different degrees of acetylation (DAs), and conjugated polymer cyano-substituted poly(p-phenylene vinylene) (CNPPV) as an alternative approach to inactivate Gram-negative (E. coli) and Gram-positive (S. aureus) bacteria. Chitosan?s structure was confirmed through FT-Raman spectroscopy. Bactericidal and photobactericidal activities of NCPs were tested under dark and blue-light irradiation conditions, respectively. Hydrodynamic size and aqueous stability were determined by DLS, zeta potential (ZP) and time-domain NMR. TEM micrographs of NCPs were obtained, and their capacity of generating reactive oxygen species(ROS) under blue illumination was also characterized. Meaningful variations on ZP and relaxationtime T2 confirmed successful physical attachment of chitosan/CNPPV. All NCPs exhibited a similarand shrunken spherical shape according to TEM. A lower DA is responsible for driving higher bactericidal performance alongside the synergistic effect from CNPPV, lower nanosized distribution profile and higher positive charged surface. ROS production was proportionally found in NCPs with and without CNPPV by decreasing the DA, leading to a remarkable photobactericidal effect underblue-light irradiation. Overall, our findings indicate that chitosan/CNPPV NCPs may constitute a valuable asset for the development of innovative strategies for inactivation and/or photoinactivation of bacteriaLUIZ ALBERTO COLNAGO, CNPDIA.FACCHINATTO, W. M.ARAUJO, L. O.MORAES, T. B.ABELHA, T. F.LIMA, T. H. N.SANTOS, D. M. dosCAMPANA-FILHO, S. P.COLNAGO, L. A.CAIRES, A. R. L.2024-01-23T10:42:05Z2024-01-23T10:42:05Z2022-11-252022info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article16 p.International Journal of Molecular Sciences, v. 23, a12519, 2022.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1148795https://doi.org/10.3390/ijms232012519enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2024-01-23T10:42:05Zoai:www.alice.cnptia.embrapa.br:doc/1148795Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542024-01-23T10:42:05falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542024-01-23T10:42:05Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Antimicrobial and Photoantimicrobial Activities of Chitosan/CNPPV Nanocomposites. |
title |
Antimicrobial and Photoantimicrobial Activities of Chitosan/CNPPV Nanocomposites. |
spellingShingle |
Antimicrobial and Photoantimicrobial Activities of Chitosan/CNPPV Nanocomposites. FACCHINATTO, W. M. Photoantimicrobial activity Blue-light irradiation CNPPV S aureus |
title_short |
Antimicrobial and Photoantimicrobial Activities of Chitosan/CNPPV Nanocomposites. |
title_full |
Antimicrobial and Photoantimicrobial Activities of Chitosan/CNPPV Nanocomposites. |
title_fullStr |
Antimicrobial and Photoantimicrobial Activities of Chitosan/CNPPV Nanocomposites. |
title_full_unstemmed |
Antimicrobial and Photoantimicrobial Activities of Chitosan/CNPPV Nanocomposites. |
title_sort |
Antimicrobial and Photoantimicrobial Activities of Chitosan/CNPPV Nanocomposites. |
author |
FACCHINATTO, W. M. |
author_facet |
FACCHINATTO, W. M. ARAUJO, L. O. MORAES, T. B. ABELHA, T. F. LIMA, T. H. N. SANTOS, D. M. dos CAMPANA-FILHO, S. P. COLNAGO, L. A. CAIRES, A. R. L. |
author_role |
author |
author2 |
ARAUJO, L. O. MORAES, T. B. ABELHA, T. F. LIMA, T. H. N. SANTOS, D. M. dos CAMPANA-FILHO, S. P. COLNAGO, L. A. CAIRES, A. R. L. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
LUIZ ALBERTO COLNAGO, CNPDIA. |
dc.contributor.author.fl_str_mv |
FACCHINATTO, W. M. ARAUJO, L. O. MORAES, T. B. ABELHA, T. F. LIMA, T. H. N. SANTOS, D. M. dos CAMPANA-FILHO, S. P. COLNAGO, L. A. CAIRES, A. R. L. |
dc.subject.por.fl_str_mv |
Photoantimicrobial activity Blue-light irradiation CNPPV S aureus |
topic |
Photoantimicrobial activity Blue-light irradiation CNPPV S aureus |
description |
Multidrug-resistant bacteria represent a global health and economic burden that urgently calls for new technologies to combat bacterial antimicrobial resistance. Here, we developed novel nanocomposites (NCPs) based on chitosan that display different degrees of acetylation (DAs), and conjugated polymer cyano-substituted poly(p-phenylene vinylene) (CNPPV) as an alternative approach to inactivate Gram-negative (E. coli) and Gram-positive (S. aureus) bacteria. Chitosan?s structure was confirmed through FT-Raman spectroscopy. Bactericidal and photobactericidal activities of NCPs were tested under dark and blue-light irradiation conditions, respectively. Hydrodynamic size and aqueous stability were determined by DLS, zeta potential (ZP) and time-domain NMR. TEM micrographs of NCPs were obtained, and their capacity of generating reactive oxygen species(ROS) under blue illumination was also characterized. Meaningful variations on ZP and relaxationtime T2 confirmed successful physical attachment of chitosan/CNPPV. All NCPs exhibited a similarand shrunken spherical shape according to TEM. A lower DA is responsible for driving higher bactericidal performance alongside the synergistic effect from CNPPV, lower nanosized distribution profile and higher positive charged surface. ROS production was proportionally found in NCPs with and without CNPPV by decreasing the DA, leading to a remarkable photobactericidal effect underblue-light irradiation. Overall, our findings indicate that chitosan/CNPPV NCPs may constitute a valuable asset for the development of innovative strategies for inactivation and/or photoinactivation of bacteria |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-11-25 2022 2024-01-23T10:42:05Z 2024-01-23T10:42:05Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
International Journal of Molecular Sciences, v. 23, a12519, 2022. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1148795 https://doi.org/10.3390/ijms232012519 |
identifier_str_mv |
International Journal of Molecular Sciences, v. 23, a12519, 2022. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1148795 https://doi.org/10.3390/ijms232012519 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
16 p. |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503556131192832 |