Análise de sequenciamento revela que pimentão é um novo hospedeiro potencial de potato leafroll virus.

• Autor(a) principal: LIMA, R. R. P. N.
• Data de Publicação: 2023
• Outros Autores: NAKASU, E. Y. T., BLAWID, R., VARGAS, O. R., SOUSA, B. M. C. de, PEREIRA, W. de S., FERREIRA, Y. F. M., INOUE-NAGATA, A. K.
• Idioma: eng
• Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
• Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1163596
• topic: Extração de RNA total; Tomate; Pimentão; Vírus; Solanum lycopersicum; Capsicum annuum var. annuum; Polerovirus; Solanum tuberosum subsp. tuberosum; Potatoes; Tomatoes

Sequencing analysis reveals that peppers is a potencial new host for potato leafroll virus. Tomato (Solanum lycopersicum) and pepper (Capsicum annuum) are among the most important vegetable crops worldwide. However, these crops are severely affected by multiple viral diseases. To survey the diversity of viral species infecting tomato and pepper plants in different regions of Brazil, symptomatic plants were sampled from the Federal District, Mato Grosso, Pernambuco, Goiás and São Paulo states. Pools of leaves were used for semi-purification of virus particles through a method of differential centrifugation. The pellets were resuspended and used for total RNA extraction, followed by standard Illumina library preparation with ribosomal RNA deletion, and sequencing.