Study of simple sequence repeat markers from coffee expressed sequences associated to leaf miner resistance.
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/881022 |
Resumo: | The objective of this work was to identify expressed simple sequence repeats (SSR) markers associated to leaf miner resistance in coffee progenies. Identification of SSR markers was accomplished by directed searches on the Brazilian Coffee Expressed Sequence Tags (EST) database. Sequence analysis of 32 selected SSR loci showed that 65% repeats are of tetra-, 21% of tri- and 14% of dinucleotides. Also, expressed SSR are localized frequently in the 5'-UTR of gene transcript. Moreover, most of the genes containing SSR are associated with defense mechanisms. Polymorphisms were analyzed in progenies segregating for resistance to the leaf miner and corresponding to advanced generations of a Coffea arabica x Coffea racemosa hybrid. Frequency of SSR alleles was 2.1 per locus. However, no polymorphism associated with leaf miner resistance was identified. These results suggest that marker-assisted selection in coffee breeding should be performed on the initial cross, in which genetic variability is still significant |
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Study of simple sequence repeat markers from coffee expressed sequences associated to leaf miner resistance.microsatellitesmolecular markersmicrossatélitesmarcadores molecularesLeucoptera CoffeellaThe objective of this work was to identify expressed simple sequence repeats (SSR) markers associated to leaf miner resistance in coffee progenies. Identification of SSR markers was accomplished by directed searches on the Brazilian Coffee Expressed Sequence Tags (EST) database. Sequence analysis of 32 selected SSR loci showed that 65% repeats are of tetra-, 21% of tri- and 14% of dinucleotides. Also, expressed SSR are localized frequently in the 5'-UTR of gene transcript. Moreover, most of the genes containing SSR are associated with defense mechanisms. Polymorphisms were analyzed in progenies segregating for resistance to the leaf miner and corresponding to advanced generations of a Coffea arabica x Coffea racemosa hybrid. Frequency of SSR alleles was 2.1 per locus. However, no polymorphism associated with leaf miner resistance was identified. These results suggest that marker-assisted selection in coffee breeding should be performed on the initial cross, in which genetic variability is still significantFERNANDA DE OLIVEIRA PINTO, IAC; MIRIAN PEREZ MALUF, SAPC; OLIVEIRO GUERREIRO-FILHO, IAC.PINTO, F. de O.MALUF, M. P.GUERREIRO-FILHO, O.2011-04-09T22:29:21Z2011-04-09T22:29:21Z2011-03-1620072011-04-10T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePesquisa Agropecuária Brasileira, Brasília, v.42, n. 3, p.377-384, mar. 2007.http://www.alice.cnptia.embrapa.br/alice/handle/doc/881022enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-15T22:32:21Zoai:www.alice.cnptia.embrapa.br:doc/881022Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-15T22:32:21falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-15T22:32:21Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Study of simple sequence repeat markers from coffee expressed sequences associated to leaf miner resistance. |
title |
Study of simple sequence repeat markers from coffee expressed sequences associated to leaf miner resistance. |
spellingShingle |
Study of simple sequence repeat markers from coffee expressed sequences associated to leaf miner resistance. PINTO, F. de O. microsatellites molecular markers microssatélites marcadores moleculares Leucoptera Coffeella |
title_short |
Study of simple sequence repeat markers from coffee expressed sequences associated to leaf miner resistance. |
title_full |
Study of simple sequence repeat markers from coffee expressed sequences associated to leaf miner resistance. |
title_fullStr |
Study of simple sequence repeat markers from coffee expressed sequences associated to leaf miner resistance. |
title_full_unstemmed |
Study of simple sequence repeat markers from coffee expressed sequences associated to leaf miner resistance. |
title_sort |
Study of simple sequence repeat markers from coffee expressed sequences associated to leaf miner resistance. |
author |
PINTO, F. de O. |
author_facet |
PINTO, F. de O. MALUF, M. P. GUERREIRO-FILHO, O. |
author_role |
author |
author2 |
MALUF, M. P. GUERREIRO-FILHO, O. |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
FERNANDA DE OLIVEIRA PINTO, IAC; MIRIAN PEREZ MALUF, SAPC; OLIVEIRO GUERREIRO-FILHO, IAC. |
dc.contributor.author.fl_str_mv |
PINTO, F. de O. MALUF, M. P. GUERREIRO-FILHO, O. |
dc.subject.por.fl_str_mv |
microsatellites molecular markers microssatélites marcadores moleculares Leucoptera Coffeella |
topic |
microsatellites molecular markers microssatélites marcadores moleculares Leucoptera Coffeella |
description |
The objective of this work was to identify expressed simple sequence repeats (SSR) markers associated to leaf miner resistance in coffee progenies. Identification of SSR markers was accomplished by directed searches on the Brazilian Coffee Expressed Sequence Tags (EST) database. Sequence analysis of 32 selected SSR loci showed that 65% repeats are of tetra-, 21% of tri- and 14% of dinucleotides. Also, expressed SSR are localized frequently in the 5'-UTR of gene transcript. Moreover, most of the genes containing SSR are associated with defense mechanisms. Polymorphisms were analyzed in progenies segregating for resistance to the leaf miner and corresponding to advanced generations of a Coffea arabica x Coffea racemosa hybrid. Frequency of SSR alleles was 2.1 per locus. However, no polymorphism associated with leaf miner resistance was identified. These results suggest that marker-assisted selection in coffee breeding should be performed on the initial cross, in which genetic variability is still significant |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007 2011-04-09T22:29:21Z 2011-04-09T22:29:21Z 2011-03-16 2011-04-10T11:11:11Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Pesquisa Agropecuária Brasileira, Brasília, v.42, n. 3, p.377-384, mar. 2007. http://www.alice.cnptia.embrapa.br/alice/handle/doc/881022 |
identifier_str_mv |
Pesquisa Agropecuária Brasileira, Brasília, v.42, n. 3, p.377-384, mar. 2007. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/881022 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503340836519936 |