Use of Ruta graveolens L. vegetable extract standardised by furanocoumarin content to control Magnaporthe oryzae in rice plants.

Detalhes bibliográficos
Autor(a) principal: ARRUDA, R. L.
Data de Publicação: 2015
Outros Autores: GARCIA, M. L., CORTES, M. V. de C. B., FILIPPI, M. C. C. de, CONCEIÇÃO, E. C. da
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1024985
Resumo: Blast, caused by the fungus Magnapor the oryzae, is one of the most widespread diseases affecting the rice crop. The use of fungicides to combat phyto pathogenic fungi causes great environmental problems. Plant extracts have been studied as an alternative method of plant pathogen control. Ruta graveolens has compounds with antifungal activity, such as furanocoumarins. The objective of this study was to test the effects of various concentrations of the standardised plant extract of R. graveolens as well as its fractions and furanocoumarins (psoralen and bergapten) on reducing M. oryzaemycelial growth. The aerial part of R. graveolens was grinded and subjected to a characterisation process, percolation, concentration, and furanocoumarin standardisation. The standardised extract was fractionated and the fractions were subjected to high-performance liquid chromatography (HPLC) to quantify furanocoumarins. The effects of the extract, fractions, and standards against mycelial growth ofM.oryzaewere tested in vitro. The plant material was handled in accordance with the guidelines established by the general methods of the Brazilian Pharmacopoeia. The furanocoumarin content was the highest in the ethyl acetate fraction. The best results for the mycelial growth test were observed using the extract and the hexane and ethyl acetate fractions. The standardised herbal extract of R. graveolens showed antifungal activity against M.oryzae.
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spelling Use of Ruta graveolens L. vegetable extract standardised by furanocoumarin content to control Magnaporthe oryzae in rice plants.Controle alternativoArrudaRuta graveolensArrozOryza sativaBrusoneDoença de plantaAntifungal proteinsPlant pathogensBlast, caused by the fungus Magnapor the oryzae, is one of the most widespread diseases affecting the rice crop. The use of fungicides to combat phyto pathogenic fungi causes great environmental problems. Plant extracts have been studied as an alternative method of plant pathogen control. Ruta graveolens has compounds with antifungal activity, such as furanocoumarins. The objective of this study was to test the effects of various concentrations of the standardised plant extract of R. graveolens as well as its fractions and furanocoumarins (psoralen and bergapten) on reducing M. oryzaemycelial growth. The aerial part of R. graveolens was grinded and subjected to a characterisation process, percolation, concentration, and furanocoumarin standardisation. The standardised extract was fractionated and the fractions were subjected to high-performance liquid chromatography (HPLC) to quantify furanocoumarins. The effects of the extract, fractions, and standards against mycelial growth ofM.oryzaewere tested in vitro. The plant material was handled in accordance with the guidelines established by the general methods of the Brazilian Pharmacopoeia. The furanocoumarin content was the highest in the ethyl acetate fraction. The best results for the mycelial growth test were observed using the extract and the hexane and ethyl acetate fractions. The standardised herbal extract of R. graveolens showed antifungal activity against M.oryzae.REJANNE LIMA ARRUDA, UFG; MYTHALI LIMA GARCIA, UFG; MARCIO VINICIUS DE C BARROS CORTES, CNPAF; MARTA CRISTINA CORSI DE FILIPPI, CNPAF; EDEMILSON CARDOSO DA CONCEIÇÃO, UFG.ARRUDA, R. L.GARCIA, M. L.CORTES, M. V. de C. B.FILIPPI, M. C. C. deCONCEIÇÃO, E. C. da2015-09-24T11:11:11Z2015-09-24T11:11:11Z2015-09-2420152016-03-29T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleInternational Journal of Research Studies in Biosciences, v. 3, n. 9, p. 94-103, Sept. 2015.2349-0365http://www.alice.cnptia.embrapa.br/alice/handle/doc/1024985enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-16T02:45:15Zoai:www.alice.cnptia.embrapa.br:doc/1024985Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-16T02:45:15falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-16T02:45:15Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Use of Ruta graveolens L. vegetable extract standardised by furanocoumarin content to control Magnaporthe oryzae in rice plants.
title Use of Ruta graveolens L. vegetable extract standardised by furanocoumarin content to control Magnaporthe oryzae in rice plants.
spellingShingle Use of Ruta graveolens L. vegetable extract standardised by furanocoumarin content to control Magnaporthe oryzae in rice plants.
ARRUDA, R. L.
Controle alternativo
Arruda
Ruta graveolens
Arroz
Oryza sativa
Brusone
Doença de planta
Antifungal proteins
Plant pathogens
title_short Use of Ruta graveolens L. vegetable extract standardised by furanocoumarin content to control Magnaporthe oryzae in rice plants.
title_full Use of Ruta graveolens L. vegetable extract standardised by furanocoumarin content to control Magnaporthe oryzae in rice plants.
title_fullStr Use of Ruta graveolens L. vegetable extract standardised by furanocoumarin content to control Magnaporthe oryzae in rice plants.
title_full_unstemmed Use of Ruta graveolens L. vegetable extract standardised by furanocoumarin content to control Magnaporthe oryzae in rice plants.
title_sort Use of Ruta graveolens L. vegetable extract standardised by furanocoumarin content to control Magnaporthe oryzae in rice plants.
author ARRUDA, R. L.
author_facet ARRUDA, R. L.
GARCIA, M. L.
CORTES, M. V. de C. B.
FILIPPI, M. C. C. de
CONCEIÇÃO, E. C. da
author_role author
author2 GARCIA, M. L.
CORTES, M. V. de C. B.
FILIPPI, M. C. C. de
CONCEIÇÃO, E. C. da
author2_role author
author
author
author
dc.contributor.none.fl_str_mv REJANNE LIMA ARRUDA, UFG; MYTHALI LIMA GARCIA, UFG; MARCIO VINICIUS DE C BARROS CORTES, CNPAF; MARTA CRISTINA CORSI DE FILIPPI, CNPAF; EDEMILSON CARDOSO DA CONCEIÇÃO, UFG.
dc.contributor.author.fl_str_mv ARRUDA, R. L.
GARCIA, M. L.
CORTES, M. V. de C. B.
FILIPPI, M. C. C. de
CONCEIÇÃO, E. C. da
dc.subject.por.fl_str_mv Controle alternativo
Arruda
Ruta graveolens
Arroz
Oryza sativa
Brusone
Doença de planta
Antifungal proteins
Plant pathogens
topic Controle alternativo
Arruda
Ruta graveolens
Arroz
Oryza sativa
Brusone
Doença de planta
Antifungal proteins
Plant pathogens
description Blast, caused by the fungus Magnapor the oryzae, is one of the most widespread diseases affecting the rice crop. The use of fungicides to combat phyto pathogenic fungi causes great environmental problems. Plant extracts have been studied as an alternative method of plant pathogen control. Ruta graveolens has compounds with antifungal activity, such as furanocoumarins. The objective of this study was to test the effects of various concentrations of the standardised plant extract of R. graveolens as well as its fractions and furanocoumarins (psoralen and bergapten) on reducing M. oryzaemycelial growth. The aerial part of R. graveolens was grinded and subjected to a characterisation process, percolation, concentration, and furanocoumarin standardisation. The standardised extract was fractionated and the fractions were subjected to high-performance liquid chromatography (HPLC) to quantify furanocoumarins. The effects of the extract, fractions, and standards against mycelial growth ofM.oryzaewere tested in vitro. The plant material was handled in accordance with the guidelines established by the general methods of the Brazilian Pharmacopoeia. The furanocoumarin content was the highest in the ethyl acetate fraction. The best results for the mycelial growth test were observed using the extract and the hexane and ethyl acetate fractions. The standardised herbal extract of R. graveolens showed antifungal activity against M.oryzae.
publishDate 2015
dc.date.none.fl_str_mv 2015-09-24T11:11:11Z
2015-09-24T11:11:11Z
2015-09-24
2015
2016-03-29T11:11:11Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv International Journal of Research Studies in Biosciences, v. 3, n. 9, p. 94-103, Sept. 2015.
2349-0365
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1024985
identifier_str_mv International Journal of Research Studies in Biosciences, v. 3, n. 9, p. 94-103, Sept. 2015.
2349-0365
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/1024985
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
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instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
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institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
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repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
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