Use of Ruta graveolens L. vegetable extract standardised by furanocoumarin content to control Magnaporthe oryzae in rice plants.
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1024985 |
Resumo: | Blast, caused by the fungus Magnapor the oryzae, is one of the most widespread diseases affecting the rice crop. The use of fungicides to combat phyto pathogenic fungi causes great environmental problems. Plant extracts have been studied as an alternative method of plant pathogen control. Ruta graveolens has compounds with antifungal activity, such as furanocoumarins. The objective of this study was to test the effects of various concentrations of the standardised plant extract of R. graveolens as well as its fractions and furanocoumarins (psoralen and bergapten) on reducing M. oryzaemycelial growth. The aerial part of R. graveolens was grinded and subjected to a characterisation process, percolation, concentration, and furanocoumarin standardisation. The standardised extract was fractionated and the fractions were subjected to high-performance liquid chromatography (HPLC) to quantify furanocoumarins. The effects of the extract, fractions, and standards against mycelial growth ofM.oryzaewere tested in vitro. The plant material was handled in accordance with the guidelines established by the general methods of the Brazilian Pharmacopoeia. The furanocoumarin content was the highest in the ethyl acetate fraction. The best results for the mycelial growth test were observed using the extract and the hexane and ethyl acetate fractions. The standardised herbal extract of R. graveolens showed antifungal activity against M.oryzae. |
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Use of Ruta graveolens L. vegetable extract standardised by furanocoumarin content to control Magnaporthe oryzae in rice plants.Controle alternativoArrudaRuta graveolensArrozOryza sativaBrusoneDoença de plantaAntifungal proteinsPlant pathogensBlast, caused by the fungus Magnapor the oryzae, is one of the most widespread diseases affecting the rice crop. The use of fungicides to combat phyto pathogenic fungi causes great environmental problems. Plant extracts have been studied as an alternative method of plant pathogen control. Ruta graveolens has compounds with antifungal activity, such as furanocoumarins. The objective of this study was to test the effects of various concentrations of the standardised plant extract of R. graveolens as well as its fractions and furanocoumarins (psoralen and bergapten) on reducing M. oryzaemycelial growth. The aerial part of R. graveolens was grinded and subjected to a characterisation process, percolation, concentration, and furanocoumarin standardisation. The standardised extract was fractionated and the fractions were subjected to high-performance liquid chromatography (HPLC) to quantify furanocoumarins. The effects of the extract, fractions, and standards against mycelial growth ofM.oryzaewere tested in vitro. The plant material was handled in accordance with the guidelines established by the general methods of the Brazilian Pharmacopoeia. The furanocoumarin content was the highest in the ethyl acetate fraction. The best results for the mycelial growth test were observed using the extract and the hexane and ethyl acetate fractions. The standardised herbal extract of R. graveolens showed antifungal activity against M.oryzae.REJANNE LIMA ARRUDA, UFG; MYTHALI LIMA GARCIA, UFG; MARCIO VINICIUS DE C BARROS CORTES, CNPAF; MARTA CRISTINA CORSI DE FILIPPI, CNPAF; EDEMILSON CARDOSO DA CONCEIÇÃO, UFG.ARRUDA, R. L.GARCIA, M. L.CORTES, M. V. de C. B.FILIPPI, M. C. C. deCONCEIÇÃO, E. C. da2015-09-24T11:11:11Z2015-09-24T11:11:11Z2015-09-2420152016-03-29T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleInternational Journal of Research Studies in Biosciences, v. 3, n. 9, p. 94-103, Sept. 2015.2349-0365http://www.alice.cnptia.embrapa.br/alice/handle/doc/1024985enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-16T02:45:15Zoai:www.alice.cnptia.embrapa.br:doc/1024985Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-16T02:45:15falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-16T02:45:15Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Use of Ruta graveolens L. vegetable extract standardised by furanocoumarin content to control Magnaporthe oryzae in rice plants. |
title |
Use of Ruta graveolens L. vegetable extract standardised by furanocoumarin content to control Magnaporthe oryzae in rice plants. |
spellingShingle |
Use of Ruta graveolens L. vegetable extract standardised by furanocoumarin content to control Magnaporthe oryzae in rice plants. ARRUDA, R. L. Controle alternativo Arruda Ruta graveolens Arroz Oryza sativa Brusone Doença de planta Antifungal proteins Plant pathogens |
title_short |
Use of Ruta graveolens L. vegetable extract standardised by furanocoumarin content to control Magnaporthe oryzae in rice plants. |
title_full |
Use of Ruta graveolens L. vegetable extract standardised by furanocoumarin content to control Magnaporthe oryzae in rice plants. |
title_fullStr |
Use of Ruta graveolens L. vegetable extract standardised by furanocoumarin content to control Magnaporthe oryzae in rice plants. |
title_full_unstemmed |
Use of Ruta graveolens L. vegetable extract standardised by furanocoumarin content to control Magnaporthe oryzae in rice plants. |
title_sort |
Use of Ruta graveolens L. vegetable extract standardised by furanocoumarin content to control Magnaporthe oryzae in rice plants. |
author |
ARRUDA, R. L. |
author_facet |
ARRUDA, R. L. GARCIA, M. L. CORTES, M. V. de C. B. FILIPPI, M. C. C. de CONCEIÇÃO, E. C. da |
author_role |
author |
author2 |
GARCIA, M. L. CORTES, M. V. de C. B. FILIPPI, M. C. C. de CONCEIÇÃO, E. C. da |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
REJANNE LIMA ARRUDA, UFG; MYTHALI LIMA GARCIA, UFG; MARCIO VINICIUS DE C BARROS CORTES, CNPAF; MARTA CRISTINA CORSI DE FILIPPI, CNPAF; EDEMILSON CARDOSO DA CONCEIÇÃO, UFG. |
dc.contributor.author.fl_str_mv |
ARRUDA, R. L. GARCIA, M. L. CORTES, M. V. de C. B. FILIPPI, M. C. C. de CONCEIÇÃO, E. C. da |
dc.subject.por.fl_str_mv |
Controle alternativo Arruda Ruta graveolens Arroz Oryza sativa Brusone Doença de planta Antifungal proteins Plant pathogens |
topic |
Controle alternativo Arruda Ruta graveolens Arroz Oryza sativa Brusone Doença de planta Antifungal proteins Plant pathogens |
description |
Blast, caused by the fungus Magnapor the oryzae, is one of the most widespread diseases affecting the rice crop. The use of fungicides to combat phyto pathogenic fungi causes great environmental problems. Plant extracts have been studied as an alternative method of plant pathogen control. Ruta graveolens has compounds with antifungal activity, such as furanocoumarins. The objective of this study was to test the effects of various concentrations of the standardised plant extract of R. graveolens as well as its fractions and furanocoumarins (psoralen and bergapten) on reducing M. oryzaemycelial growth. The aerial part of R. graveolens was grinded and subjected to a characterisation process, percolation, concentration, and furanocoumarin standardisation. The standardised extract was fractionated and the fractions were subjected to high-performance liquid chromatography (HPLC) to quantify furanocoumarins. The effects of the extract, fractions, and standards against mycelial growth ofM.oryzaewere tested in vitro. The plant material was handled in accordance with the guidelines established by the general methods of the Brazilian Pharmacopoeia. The furanocoumarin content was the highest in the ethyl acetate fraction. The best results for the mycelial growth test were observed using the extract and the hexane and ethyl acetate fractions. The standardised herbal extract of R. graveolens showed antifungal activity against M.oryzae. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-09-24T11:11:11Z 2015-09-24T11:11:11Z 2015-09-24 2015 2016-03-29T11:11:11Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
International Journal of Research Studies in Biosciences, v. 3, n. 9, p. 94-103, Sept. 2015. 2349-0365 http://www.alice.cnptia.embrapa.br/alice/handle/doc/1024985 |
identifier_str_mv |
International Journal of Research Studies in Biosciences, v. 3, n. 9, p. 94-103, Sept. 2015. 2349-0365 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1024985 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
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instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
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EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503412077821952 |