Development and characterization of new microsatellites for Eugenia dysenterica DC (Myrtaceae).

• Autor(a) principal: TELLES, M. P. C.
• Data de Publicação: 2013
• Outros Autores: SILVA, J. B., RESENDE, L. V., VIANELLO, R. P., CHAVES, L. J., SOARES, T. N., COLLEVATTI, R. G.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
• Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/978390; http://dx.doi.org/10.4238/2013.February.6.3
• topic: Fruta tropical; Cerrado; Cagaita; Marcador molecular; Variação genética; Genetic variation; Microsatellite repeats

Microsatellite markers were developed for population genetic analyses of the Neotropical tree Eugenia dysenterica DC (Myrtaceae), after construction of a shotgun genomic library for microsatellite discovery. Nine primers were designed, of which 5 yielded amplified product. These primers were polymorphic for 97 individuals collected in 3 distinct localities. The number of alleles per locus (primer) ranged from 3 to 11 and expected heterozygosities varied from 0.309 to 0.884. The probability of locus identity was ~1.88 x 10-4 and the probability of paternity exclusion was ~0.9367. The 5 microsatellite primer pairs may be suitable for population genetic studies such as parentage and fine-scale genetic analyses of this species.