Comparison of eight methods to isolate genomic DNA from Hancornia speciosa.
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1073486 http://dx.doi.org/10.4238/gmr16039724 |
Resumo: | Mangabeira (Hancornia speciosa) is a native fruit tree found mainly in the Cerrado biome and shows great economic potential due to its multiple uses; the fruits are used in agriculture, are important as a food resource, and can be consumed in natura or processed. Due to a reduction in the area of ecosystems where it occurs, mangabeira is threatened by genetic erosion in Brazil. The characterization of the genetic diversity of plants can provide the basis for strategies to protect and conserve endangered populations, like mangabeira. This study aimed to compare eight DNA extraction methods in mangabeira because the key to success is the use of a pure genomic DNA for the characterization of genetic diversity in molecular biology techniques. The quality and concentration of DNA were revealed by agarose gel electrophoresis. Polymerase chain reaction amplifications were successfully by extractions using two commercial purification kits and by the method proposed by Khanuja et al. (1999), which produced sufficient genomic DNA of good quality from leaves of H. speciosa to perform techniques involving molecular biology. The protocol described by Khanuja et al. (1999) is less expensive when compared to the commercial purification kits. |
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Comparison of eight methods to isolate genomic DNA from Hancornia speciosa.Isolamento do DNAMangabeiraReação em cadeia da polimeraseDNA isolationpolymerase chain reactionMangabeira (Hancornia speciosa) is a native fruit tree found mainly in the Cerrado biome and shows great economic potential due to its multiple uses; the fruits are used in agriculture, are important as a food resource, and can be consumed in natura or processed. Due to a reduction in the area of ecosystems where it occurs, mangabeira is threatened by genetic erosion in Brazil. The characterization of the genetic diversity of plants can provide the basis for strategies to protect and conserve endangered populations, like mangabeira. This study aimed to compare eight DNA extraction methods in mangabeira because the key to success is the use of a pure genomic DNA for the characterization of genetic diversity in molecular biology techniques. The quality and concentration of DNA were revealed by agarose gel electrophoresis. Polymerase chain reaction amplifications were successfully by extractions using two commercial purification kits and by the method proposed by Khanuja et al. (1999), which produced sufficient genomic DNA of good quality from leaves of H. speciosa to perform techniques involving molecular biology. The protocol described by Khanuja et al. (1999) is less expensive when compared to the commercial purification kits.UFPI; UFPI; UFPI; UFPI; UFPI; PAULO SARMANHO DA COSTA LIMA, CPAMN; UFPI.ALMEIDA, V. M.LUZ, G. A.MARTINS, P. P.GOMES, M. F. C.COSTA, M. F.LIMA, P. S. da C.VALENTE, S. E. S.2017-08-02T11:11:11Z2017-08-02T11:11:11Z2017-08-0220172017-08-02T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article7 p.Genetics and Molecular Research, Ribeirão Preto, v.16, n. 3: gmr16039724, 2017.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1073486http://dx.doi.org/10.4238/gmr16039724enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-16T02:33:08Zoai:www.alice.cnptia.embrapa.br:doc/1073486Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-16T02:33:08falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-16T02:33:08Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Comparison of eight methods to isolate genomic DNA from Hancornia speciosa. |
title |
Comparison of eight methods to isolate genomic DNA from Hancornia speciosa. |
spellingShingle |
Comparison of eight methods to isolate genomic DNA from Hancornia speciosa. ALMEIDA, V. M. Isolamento do DNA Mangabeira Reação em cadeia da polimerase DNA isolation polymerase chain reaction |
title_short |
Comparison of eight methods to isolate genomic DNA from Hancornia speciosa. |
title_full |
Comparison of eight methods to isolate genomic DNA from Hancornia speciosa. |
title_fullStr |
Comparison of eight methods to isolate genomic DNA from Hancornia speciosa. |
title_full_unstemmed |
Comparison of eight methods to isolate genomic DNA from Hancornia speciosa. |
title_sort |
Comparison of eight methods to isolate genomic DNA from Hancornia speciosa. |
author |
ALMEIDA, V. M. |
author_facet |
ALMEIDA, V. M. LUZ, G. A. MARTINS, P. P. GOMES, M. F. C. COSTA, M. F. LIMA, P. S. da C. VALENTE, S. E. S. |
author_role |
author |
author2 |
LUZ, G. A. MARTINS, P. P. GOMES, M. F. C. COSTA, M. F. LIMA, P. S. da C. VALENTE, S. E. S. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
UFPI; UFPI; UFPI; UFPI; UFPI; PAULO SARMANHO DA COSTA LIMA, CPAMN; UFPI. |
dc.contributor.author.fl_str_mv |
ALMEIDA, V. M. LUZ, G. A. MARTINS, P. P. GOMES, M. F. C. COSTA, M. F. LIMA, P. S. da C. VALENTE, S. E. S. |
dc.subject.por.fl_str_mv |
Isolamento do DNA Mangabeira Reação em cadeia da polimerase DNA isolation polymerase chain reaction |
topic |
Isolamento do DNA Mangabeira Reação em cadeia da polimerase DNA isolation polymerase chain reaction |
description |
Mangabeira (Hancornia speciosa) is a native fruit tree found mainly in the Cerrado biome and shows great economic potential due to its multiple uses; the fruits are used in agriculture, are important as a food resource, and can be consumed in natura or processed. Due to a reduction in the area of ecosystems where it occurs, mangabeira is threatened by genetic erosion in Brazil. The characterization of the genetic diversity of plants can provide the basis for strategies to protect and conserve endangered populations, like mangabeira. This study aimed to compare eight DNA extraction methods in mangabeira because the key to success is the use of a pure genomic DNA for the characterization of genetic diversity in molecular biology techniques. The quality and concentration of DNA were revealed by agarose gel electrophoresis. Polymerase chain reaction amplifications were successfully by extractions using two commercial purification kits and by the method proposed by Khanuja et al. (1999), which produced sufficient genomic DNA of good quality from leaves of H. speciosa to perform techniques involving molecular biology. The protocol described by Khanuja et al. (1999) is less expensive when compared to the commercial purification kits. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-08-02T11:11:11Z 2017-08-02T11:11:11Z 2017-08-02 2017 2017-08-02T11:11:11Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Genetics and Molecular Research, Ribeirão Preto, v.16, n. 3: gmr16039724, 2017. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1073486 http://dx.doi.org/10.4238/gmr16039724 |
identifier_str_mv |
Genetics and Molecular Research, Ribeirão Preto, v.16, n. 3: gmr16039724, 2017. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1073486 http://dx.doi.org/10.4238/gmr16039724 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
7 p. |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503440152395776 |