Evoluation of the proleferation of dedifferentiated cells of yellow bell pepper (Caapsicum annuum cv. Pimentao amarelo).
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1080930 |
Resumo: | Capsicum annuum is notable for its secondary metabolites, which can be of great interest for use in agriculture as alternatives to agrochemicals. Cell suspensions have been used for in vitro largescale production of secondary metabolites. The aim of this study was to dedifferentiate leaf cells of Capsicum annuum cv. Pimentão Amarelo (yellow bell pepper) into calluses, and to study their growth pattern, focusing on the deceleration phase, when they can be subcultured to establish cell suspensions. Leaf explants were inoculated on MS medium supplemented with the growth regulators 2,4-D (0, 4.52, 9.05 and 18.10 µM) and BA (0, 4.44, 8.88 and 17.76 µM) in factorial combinations. Callus formation was evaluated weekly for 42 days, by assessing the number of callus induced per treatment and the weight of the explants. The culture medium supplemented with 4.52 µM 2,4-D and 4.44 µM BA resulted in the highest callus induction and weight of callus. For the identification of the growth curve the explants were submitted to this combination and in the subsequent 42 days, every seven days, the weight of the calluses was periodically measured. The growth curve has a sigmoid pattern and the deceleration phase begins 21 days after inoculation. |
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Evoluation of the proleferation of dedifferentiated cells of yellow bell pepper (Caapsicum annuum cv. Pimentao amarelo).SolanaceaseCallusessecondary metabolitesCapsicum annuum is notable for its secondary metabolites, which can be of great interest for use in agriculture as alternatives to agrochemicals. Cell suspensions have been used for in vitro largescale production of secondary metabolites. The aim of this study was to dedifferentiate leaf cells of Capsicum annuum cv. Pimentão Amarelo (yellow bell pepper) into calluses, and to study their growth pattern, focusing on the deceleration phase, when they can be subcultured to establish cell suspensions. Leaf explants were inoculated on MS medium supplemented with the growth regulators 2,4-D (0, 4.52, 9.05 and 18.10 µM) and BA (0, 4.44, 8.88 and 17.76 µM) in factorial combinations. Callus formation was evaluated weekly for 42 days, by assessing the number of callus induced per treatment and the weight of the explants. The culture medium supplemented with 4.52 µM 2,4-D and 4.44 µM BA resulted in the highest callus induction and weight of callus. For the identification of the growth curve the explants were submitted to this combination and in the subsequent 42 days, every seven days, the weight of the calluses was periodically measured. The growth curve has a sigmoid pattern and the deceleration phase begins 21 days after inoculation.MAURICIO REGINALDO ALVES DOS SANTOS, CPAF-Rondonia.SANTOS, M. R. A. dosSOUZA, P. G.2017-11-28T23:28:20Z2017-11-28T23:28:20Z2017-11-2820172017-11-29T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleInternational Journal of Development Research, v. 7, n. 8, p.14566-14569, Aug. 2017.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1080930enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-11-28T23:28:26Zoai:www.alice.cnptia.embrapa.br:doc/1080930Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-11-28T23:28:26falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-11-28T23:28:26Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Evoluation of the proleferation of dedifferentiated cells of yellow bell pepper (Caapsicum annuum cv. Pimentao amarelo). |
title |
Evoluation of the proleferation of dedifferentiated cells of yellow bell pepper (Caapsicum annuum cv. Pimentao amarelo). |
spellingShingle |
Evoluation of the proleferation of dedifferentiated cells of yellow bell pepper (Caapsicum annuum cv. Pimentao amarelo). SANTOS, M. R. A. dos Solanacease Calluses secondary metabolites |
title_short |
Evoluation of the proleferation of dedifferentiated cells of yellow bell pepper (Caapsicum annuum cv. Pimentao amarelo). |
title_full |
Evoluation of the proleferation of dedifferentiated cells of yellow bell pepper (Caapsicum annuum cv. Pimentao amarelo). |
title_fullStr |
Evoluation of the proleferation of dedifferentiated cells of yellow bell pepper (Caapsicum annuum cv. Pimentao amarelo). |
title_full_unstemmed |
Evoluation of the proleferation of dedifferentiated cells of yellow bell pepper (Caapsicum annuum cv. Pimentao amarelo). |
title_sort |
Evoluation of the proleferation of dedifferentiated cells of yellow bell pepper (Caapsicum annuum cv. Pimentao amarelo). |
author |
SANTOS, M. R. A. dos |
author_facet |
SANTOS, M. R. A. dos SOUZA, P. G. |
author_role |
author |
author2 |
SOUZA, P. G. |
author2_role |
author |
dc.contributor.none.fl_str_mv |
MAURICIO REGINALDO ALVES DOS SANTOS, CPAF-Rondonia. |
dc.contributor.author.fl_str_mv |
SANTOS, M. R. A. dos SOUZA, P. G. |
dc.subject.por.fl_str_mv |
Solanacease Calluses secondary metabolites |
topic |
Solanacease Calluses secondary metabolites |
description |
Capsicum annuum is notable for its secondary metabolites, which can be of great interest for use in agriculture as alternatives to agrochemicals. Cell suspensions have been used for in vitro largescale production of secondary metabolites. The aim of this study was to dedifferentiate leaf cells of Capsicum annuum cv. Pimentão Amarelo (yellow bell pepper) into calluses, and to study their growth pattern, focusing on the deceleration phase, when they can be subcultured to establish cell suspensions. Leaf explants were inoculated on MS medium supplemented with the growth regulators 2,4-D (0, 4.52, 9.05 and 18.10 µM) and BA (0, 4.44, 8.88 and 17.76 µM) in factorial combinations. Callus formation was evaluated weekly for 42 days, by assessing the number of callus induced per treatment and the weight of the explants. The culture medium supplemented with 4.52 µM 2,4-D and 4.44 µM BA resulted in the highest callus induction and weight of callus. For the identification of the growth curve the explants were submitted to this combination and in the subsequent 42 days, every seven days, the weight of the calluses was periodically measured. The growth curve has a sigmoid pattern and the deceleration phase begins 21 days after inoculation. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-11-28T23:28:20Z 2017-11-28T23:28:20Z 2017-11-28 2017 2017-11-29T11:11:11Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
International Journal of Development Research, v. 7, n. 8, p.14566-14569, Aug. 2017. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1080930 |
identifier_str_mv |
International Journal of Development Research, v. 7, n. 8, p.14566-14569, Aug. 2017. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1080930 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503445536833536 |