Population genetic structure of the coffee pathogen Hemileia vastatrix in Minas Gerais, Brazil.
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1125253 https://doi.org/10.1007/s40858-018-0246-9 |
Resumo: | The biotrophic fungus Hemileia vastatrix Berk & Broome is the most destructive coffee pathogen in Brazil. Better understanding of the population genetics of H. vastatrix would provide important insights into its biology, epidemiology, and evolutionary potential. The aim of the present study was to assess the genetic diversity and population structure of H. vastatrix in Minas Gerais (Brazil) using ribosomal DNA (rDNA) sequences. The analyzes were performed by sequencing the internal transcribed spacers ITS1 and ITS2, and the 5.8S gene from 15 H. vastatrix populations. Of the 82 sequences obtained, 68 ribotypes were found, as defined by 108 nucleotide substitutions and five indels. Of the 68 ribotypes, 64 were exclusively found in one population. Analysis of molecular variance (AMOVA) and FST fixation index indicated moderate genetic differentiation among field populations, which were divided according to geographic origin. In conclusion, analysis of the nuclear ITS1?5.8S-ITS2 rDNA sequence diversity in the H. vastatrix population of Minas Gerais revealed that most ribotypes are restricted to a single population and that there exists greater genetic diversity within than among field populations. |
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Population genetic structure of the coffee pathogen Hemileia vastatrix in Minas Gerais, Brazil.Coffee rustITS polymorphismCoffea ArábicaPopulation structureThe biotrophic fungus Hemileia vastatrix Berk & Broome is the most destructive coffee pathogen in Brazil. Better understanding of the population genetics of H. vastatrix would provide important insights into its biology, epidemiology, and evolutionary potential. The aim of the present study was to assess the genetic diversity and population structure of H. vastatrix in Minas Gerais (Brazil) using ribosomal DNA (rDNA) sequences. The analyzes were performed by sequencing the internal transcribed spacers ITS1 and ITS2, and the 5.8S gene from 15 H. vastatrix populations. Of the 82 sequences obtained, 68 ribotypes were found, as defined by 108 nucleotide substitutions and five indels. Of the 68 ribotypes, 64 were exclusively found in one population. Analysis of molecular variance (AMOVA) and FST fixation index indicated moderate genetic differentiation among field populations, which were divided according to geographic origin. In conclusion, analysis of the nuclear ITS1?5.8S-ITS2 rDNA sequence diversity in the H. vastatrix population of Minas Gerais revealed that most ribotypes are restricted to a single population and that there exists greater genetic diversity within than among field populations.Mateus F. Santana, Universidade Federal de Viçosa; Eunize M. Zambolim, Universidade Federal de Viçosa; EVELINE TEIXEIRA CAIXETA MOURA, CNPCa; Laércio Zambolim, Universidade Federal de Viçosa.SANTANA, M. F.ZAMBOLIM, E. M.CAIXETA, E. T.ZAMBOLIM, L.2020-10-08T09:13:15Z2020-10-08T09:13:15Z2020-10-022018info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleTropical Plant Pathology, v. 43, n. 5 , p. 473-476, 2018.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1125253https://doi.org/10.1007/s40858-018-0246-9enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2020-10-08T09:13:22Zoai:www.alice.cnptia.embrapa.br:doc/1125253Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542020-10-08T09:13:22Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Population genetic structure of the coffee pathogen Hemileia vastatrix in Minas Gerais, Brazil. |
title |
Population genetic structure of the coffee pathogen Hemileia vastatrix in Minas Gerais, Brazil. |
spellingShingle |
Population genetic structure of the coffee pathogen Hemileia vastatrix in Minas Gerais, Brazil. SANTANA, M. F. Coffee rust ITS polymorphism Coffea Arábica Population structure |
title_short |
Population genetic structure of the coffee pathogen Hemileia vastatrix in Minas Gerais, Brazil. |
title_full |
Population genetic structure of the coffee pathogen Hemileia vastatrix in Minas Gerais, Brazil. |
title_fullStr |
Population genetic structure of the coffee pathogen Hemileia vastatrix in Minas Gerais, Brazil. |
title_full_unstemmed |
Population genetic structure of the coffee pathogen Hemileia vastatrix in Minas Gerais, Brazil. |
title_sort |
Population genetic structure of the coffee pathogen Hemileia vastatrix in Minas Gerais, Brazil. |
author |
SANTANA, M. F. |
author_facet |
SANTANA, M. F. ZAMBOLIM, E. M. CAIXETA, E. T. ZAMBOLIM, L. |
author_role |
author |
author2 |
ZAMBOLIM, E. M. CAIXETA, E. T. ZAMBOLIM, L. |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Mateus F. Santana, Universidade Federal de Viçosa; Eunize M. Zambolim, Universidade Federal de Viçosa; EVELINE TEIXEIRA CAIXETA MOURA, CNPCa; Laércio Zambolim, Universidade Federal de Viçosa. |
dc.contributor.author.fl_str_mv |
SANTANA, M. F. ZAMBOLIM, E. M. CAIXETA, E. T. ZAMBOLIM, L. |
dc.subject.por.fl_str_mv |
Coffee rust ITS polymorphism Coffea Arábica Population structure |
topic |
Coffee rust ITS polymorphism Coffea Arábica Population structure |
description |
The biotrophic fungus Hemileia vastatrix Berk & Broome is the most destructive coffee pathogen in Brazil. Better understanding of the population genetics of H. vastatrix would provide important insights into its biology, epidemiology, and evolutionary potential. The aim of the present study was to assess the genetic diversity and population structure of H. vastatrix in Minas Gerais (Brazil) using ribosomal DNA (rDNA) sequences. The analyzes were performed by sequencing the internal transcribed spacers ITS1 and ITS2, and the 5.8S gene from 15 H. vastatrix populations. Of the 82 sequences obtained, 68 ribotypes were found, as defined by 108 nucleotide substitutions and five indels. Of the 68 ribotypes, 64 were exclusively found in one population. Analysis of molecular variance (AMOVA) and FST fixation index indicated moderate genetic differentiation among field populations, which were divided according to geographic origin. In conclusion, analysis of the nuclear ITS1?5.8S-ITS2 rDNA sequence diversity in the H. vastatrix population of Minas Gerais revealed that most ribotypes are restricted to a single population and that there exists greater genetic diversity within than among field populations. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018 2020-10-08T09:13:15Z 2020-10-08T09:13:15Z 2020-10-02 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Tropical Plant Pathology, v. 43, n. 5 , p. 473-476, 2018. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1125253 https://doi.org/10.1007/s40858-018-0246-9 |
identifier_str_mv |
Tropical Plant Pathology, v. 43, n. 5 , p. 473-476, 2018. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1125253 https://doi.org/10.1007/s40858-018-0246-9 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
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Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
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EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1817695597036044288 |