Molecular studies of the brazilian infectious bronchitis virus isolates.
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/865545 |
Resumo: | Avian infectious bronchitis virus (IBV) isolates have been widely characterized by reverse transcription followed by polymerase chain reaction and DNA sequencing. In present study, these techniques were applied to three viral genomic regions comprising the complete and/or a partial S1 segment, S2 and nucleocapsid genes. DNA sequences from viral isolates obtained from 1972 to 1989 and from 2006 to 2008 were compared. High similarity (>90%) was observed among some of the genomic segments, including S1 hypervariable region, which could suggest a common origin or ancestry. DNA sequences from S2 and N protein genes obtained from different infected tissues of the same flock were analyzed, and a clear segregation between respiratory and intestinal tract was observed. Therefore, these data suggest cocirculation of more than one viral strain in the same flock. 57.1% of DNA sequences from the S1 complete segment samples, 53.3% from the S2 fragment and 62.5% from the partial N gene were found to be different from analyzed sequences from reference strains leading to the conclusion that parte of viral isolates included in this study may be considered region specific. Considering the simultaneous analysis of the three genes, a large IBV genetic profile was observed in both old and recent isolates groups. However, most prominent diversity between viral isolates was obtained in the period from 1972 and 1989, showing the presence of a large number of variants in the state of Minas Gerais before the official approval of vaccination (1980). |
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Molecular studies of the brazilian infectious bronchitis virus isolates.GenéticaVirusAvian infectious bronchitis virus (IBV) isolates have been widely characterized by reverse transcription followed by polymerase chain reaction and DNA sequencing. In present study, these techniques were applied to three viral genomic regions comprising the complete and/or a partial S1 segment, S2 and nucleocapsid genes. DNA sequences from viral isolates obtained from 1972 to 1989 and from 2006 to 2008 were compared. High similarity (>90%) was observed among some of the genomic segments, including S1 hypervariable region, which could suggest a common origin or ancestry. DNA sequences from S2 and N protein genes obtained from different infected tissues of the same flock were analyzed, and a clear segregation between respiratory and intestinal tract was observed. Therefore, these data suggest cocirculation of more than one viral strain in the same flock. 57.1% of DNA sequences from the S1 complete segment samples, 53.3% from the S2 fragment and 62.5% from the partial N gene were found to be different from analyzed sequences from reference strains leading to the conclusion that parte of viral isolates included in this study may be considered region specific. Considering the simultaneous analysis of the three genes, a large IBV genetic profile was observed in both old and recent isolates groups. However, most prominent diversity between viral isolates was obtained in the period from 1972 and 1989, showing the presence of a large number of variants in the state of Minas Gerais before the official approval of vaccination (1980).AUREA VALADARES FOLGUERAS FLATSCHAR, CNPMS.ABREU, J. T.MOURÃO, M. M.SANTOS, C. E.VELOSO, C. J. M.RESENDE, J. S.FLATSCHART, R. B.FOLGUERAS-FLATSCHAR, A. V.JUNIOR, S. N.SANTORO, M. M.MENDES, A. C. R.FRANCO, G. R.SILVA, A.CAMPOS, A. B.FERNANDEZ, S.2011-04-10T11:11:11Z2011-04-10T11:11:11Z2010-10-2820102018-05-28T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleRevista Brasileira de Ciência Avícola, Campinas, v. 12, n. 2, p. 107-110, 2010.http://www.alice.cnptia.embrapa.br/alice/handle/doc/86554510.1590/S1516-635X2010000200005enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-15T22:15:53Zoai:www.alice.cnptia.embrapa.br:doc/865545Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-15T22:15:53Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Molecular studies of the brazilian infectious bronchitis virus isolates. |
title |
Molecular studies of the brazilian infectious bronchitis virus isolates. |
spellingShingle |
Molecular studies of the brazilian infectious bronchitis virus isolates. ABREU, J. T. Genética Virus |
title_short |
Molecular studies of the brazilian infectious bronchitis virus isolates. |
title_full |
Molecular studies of the brazilian infectious bronchitis virus isolates. |
title_fullStr |
Molecular studies of the brazilian infectious bronchitis virus isolates. |
title_full_unstemmed |
Molecular studies of the brazilian infectious bronchitis virus isolates. |
title_sort |
Molecular studies of the brazilian infectious bronchitis virus isolates. |
author |
ABREU, J. T. |
author_facet |
ABREU, J. T. MOURÃO, M. M. SANTOS, C. E. VELOSO, C. J. M. RESENDE, J. S. FLATSCHART, R. B. FOLGUERAS-FLATSCHAR, A. V. JUNIOR, S. N. SANTORO, M. M. MENDES, A. C. R. FRANCO, G. R. SILVA, A. CAMPOS, A. B. FERNANDEZ, S. |
author_role |
author |
author2 |
MOURÃO, M. M. SANTOS, C. E. VELOSO, C. J. M. RESENDE, J. S. FLATSCHART, R. B. FOLGUERAS-FLATSCHAR, A. V. JUNIOR, S. N. SANTORO, M. M. MENDES, A. C. R. FRANCO, G. R. SILVA, A. CAMPOS, A. B. FERNANDEZ, S. |
author2_role |
author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
AUREA VALADARES FOLGUERAS FLATSCHAR, CNPMS. |
dc.contributor.author.fl_str_mv |
ABREU, J. T. MOURÃO, M. M. SANTOS, C. E. VELOSO, C. J. M. RESENDE, J. S. FLATSCHART, R. B. FOLGUERAS-FLATSCHAR, A. V. JUNIOR, S. N. SANTORO, M. M. MENDES, A. C. R. FRANCO, G. R. SILVA, A. CAMPOS, A. B. FERNANDEZ, S. |
dc.subject.por.fl_str_mv |
Genética Virus |
topic |
Genética Virus |
description |
Avian infectious bronchitis virus (IBV) isolates have been widely characterized by reverse transcription followed by polymerase chain reaction and DNA sequencing. In present study, these techniques were applied to three viral genomic regions comprising the complete and/or a partial S1 segment, S2 and nucleocapsid genes. DNA sequences from viral isolates obtained from 1972 to 1989 and from 2006 to 2008 were compared. High similarity (>90%) was observed among some of the genomic segments, including S1 hypervariable region, which could suggest a common origin or ancestry. DNA sequences from S2 and N protein genes obtained from different infected tissues of the same flock were analyzed, and a clear segregation between respiratory and intestinal tract was observed. Therefore, these data suggest cocirculation of more than one viral strain in the same flock. 57.1% of DNA sequences from the S1 complete segment samples, 53.3% from the S2 fragment and 62.5% from the partial N gene were found to be different from analyzed sequences from reference strains leading to the conclusion that parte of viral isolates included in this study may be considered region specific. Considering the simultaneous analysis of the three genes, a large IBV genetic profile was observed in both old and recent isolates groups. However, most prominent diversity between viral isolates was obtained in the period from 1972 and 1989, showing the presence of a large number of variants in the state of Minas Gerais before the official approval of vaccination (1980). |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-10-28 2010 2011-04-10T11:11:11Z 2011-04-10T11:11:11Z 2018-05-28T11:11:11Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Revista Brasileira de Ciência Avícola, Campinas, v. 12, n. 2, p. 107-110, 2010. http://www.alice.cnptia.embrapa.br/alice/handle/doc/865545 10.1590/S1516-635X2010000200005 |
identifier_str_mv |
Revista Brasileira de Ciência Avícola, Campinas, v. 12, n. 2, p. 107-110, 2010. 10.1590/S1516-635X2010000200005 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/865545 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1817695146578280448 |