Molecular studies of the brazilian infectious bronchitis virus isolates.

Detalhes bibliográficos
Autor(a) principal: ABREU, J. T.
Data de Publicação: 2010
Outros Autores: MOURÃO, M. M., SANTOS, C. E., VELOSO, C. J. M., RESENDE, J. S., FLATSCHART, R. B., FOLGUERAS-FLATSCHAR, A. V., JUNIOR, S. N., SANTORO, M. M., MENDES, A. C. R., FRANCO, G. R., SILVA, A., CAMPOS, A. B., FERNANDEZ, S.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/865545
Resumo: Avian infectious bronchitis virus (IBV) isolates have been widely characterized by reverse transcription followed by polymerase chain reaction and DNA sequencing. In present study, these techniques were applied to three viral genomic regions comprising the complete and/or a partial S1 segment, S2 and nucleocapsid genes. DNA sequences from viral isolates obtained from 1972 to 1989 and from 2006 to 2008 were compared. High similarity (>90%) was observed among some of the genomic segments, including S1 hypervariable region, which could suggest a common origin or ancestry. DNA sequences from S2 and N protein genes obtained from different infected tissues of the same flock were analyzed, and a clear segregation between respiratory and intestinal tract was observed. Therefore, these data suggest cocirculation of more than one viral strain in the same flock. 57.1% of DNA sequences from the S1 complete segment samples, 53.3% from the S2 fragment and 62.5% from the partial N gene were found to be different from analyzed sequences from reference strains leading to the conclusion that parte of viral isolates included in this study may be considered region specific. Considering the simultaneous analysis of the three genes, a large IBV genetic profile was observed in both old and recent isolates groups. However, most prominent diversity between viral isolates was obtained in the period from 1972 and 1989, showing the presence of a large number of variants in the state of Minas Gerais before the official approval of vaccination (1980).
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spelling Molecular studies of the brazilian infectious bronchitis virus isolates.GenéticaVirusAvian infectious bronchitis virus (IBV) isolates have been widely characterized by reverse transcription followed by polymerase chain reaction and DNA sequencing. In present study, these techniques were applied to three viral genomic regions comprising the complete and/or a partial S1 segment, S2 and nucleocapsid genes. DNA sequences from viral isolates obtained from 1972 to 1989 and from 2006 to 2008 were compared. High similarity (>90%) was observed among some of the genomic segments, including S1 hypervariable region, which could suggest a common origin or ancestry. DNA sequences from S2 and N protein genes obtained from different infected tissues of the same flock were analyzed, and a clear segregation between respiratory and intestinal tract was observed. Therefore, these data suggest cocirculation of more than one viral strain in the same flock. 57.1% of DNA sequences from the S1 complete segment samples, 53.3% from the S2 fragment and 62.5% from the partial N gene were found to be different from analyzed sequences from reference strains leading to the conclusion that parte of viral isolates included in this study may be considered region specific. Considering the simultaneous analysis of the three genes, a large IBV genetic profile was observed in both old and recent isolates groups. However, most prominent diversity between viral isolates was obtained in the period from 1972 and 1989, showing the presence of a large number of variants in the state of Minas Gerais before the official approval of vaccination (1980).AUREA VALADARES FOLGUERAS FLATSCHAR, CNPMS.ABREU, J. T.MOURÃO, M. M.SANTOS, C. E.VELOSO, C. J. M.RESENDE, J. S.FLATSCHART, R. B.FOLGUERAS-FLATSCHAR, A. V.JUNIOR, S. N.SANTORO, M. M.MENDES, A. C. R.FRANCO, G. R.SILVA, A.CAMPOS, A. B.FERNANDEZ, S.2011-04-10T11:11:11Z2011-04-10T11:11:11Z2010-10-2820102018-05-28T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleRevista Brasileira de Ciência Avícola, Campinas, v. 12, n. 2, p. 107-110, 2010.http://www.alice.cnptia.embrapa.br/alice/handle/doc/86554510.1590/S1516-635X2010000200005enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-15T22:15:53Zoai:www.alice.cnptia.embrapa.br:doc/865545Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-15T22:15:53Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Molecular studies of the brazilian infectious bronchitis virus isolates.
title Molecular studies of the brazilian infectious bronchitis virus isolates.
spellingShingle Molecular studies of the brazilian infectious bronchitis virus isolates.
ABREU, J. T.
Genética
Virus
title_short Molecular studies of the brazilian infectious bronchitis virus isolates.
title_full Molecular studies of the brazilian infectious bronchitis virus isolates.
title_fullStr Molecular studies of the brazilian infectious bronchitis virus isolates.
title_full_unstemmed Molecular studies of the brazilian infectious bronchitis virus isolates.
title_sort Molecular studies of the brazilian infectious bronchitis virus isolates.
author ABREU, J. T.
author_facet ABREU, J. T.
MOURÃO, M. M.
SANTOS, C. E.
VELOSO, C. J. M.
RESENDE, J. S.
FLATSCHART, R. B.
FOLGUERAS-FLATSCHAR, A. V.
JUNIOR, S. N.
SANTORO, M. M.
MENDES, A. C. R.
FRANCO, G. R.
SILVA, A.
CAMPOS, A. B.
FERNANDEZ, S.
author_role author
author2 MOURÃO, M. M.
SANTOS, C. E.
VELOSO, C. J. M.
RESENDE, J. S.
FLATSCHART, R. B.
FOLGUERAS-FLATSCHAR, A. V.
JUNIOR, S. N.
SANTORO, M. M.
MENDES, A. C. R.
FRANCO, G. R.
SILVA, A.
CAMPOS, A. B.
FERNANDEZ, S.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv AUREA VALADARES FOLGUERAS FLATSCHAR, CNPMS.
dc.contributor.author.fl_str_mv ABREU, J. T.
MOURÃO, M. M.
SANTOS, C. E.
VELOSO, C. J. M.
RESENDE, J. S.
FLATSCHART, R. B.
FOLGUERAS-FLATSCHAR, A. V.
JUNIOR, S. N.
SANTORO, M. M.
MENDES, A. C. R.
FRANCO, G. R.
SILVA, A.
CAMPOS, A. B.
FERNANDEZ, S.
dc.subject.por.fl_str_mv Genética
Virus
topic Genética
Virus
description Avian infectious bronchitis virus (IBV) isolates have been widely characterized by reverse transcription followed by polymerase chain reaction and DNA sequencing. In present study, these techniques were applied to three viral genomic regions comprising the complete and/or a partial S1 segment, S2 and nucleocapsid genes. DNA sequences from viral isolates obtained from 1972 to 1989 and from 2006 to 2008 were compared. High similarity (>90%) was observed among some of the genomic segments, including S1 hypervariable region, which could suggest a common origin or ancestry. DNA sequences from S2 and N protein genes obtained from different infected tissues of the same flock were analyzed, and a clear segregation between respiratory and intestinal tract was observed. Therefore, these data suggest cocirculation of more than one viral strain in the same flock. 57.1% of DNA sequences from the S1 complete segment samples, 53.3% from the S2 fragment and 62.5% from the partial N gene were found to be different from analyzed sequences from reference strains leading to the conclusion that parte of viral isolates included in this study may be considered region specific. Considering the simultaneous analysis of the three genes, a large IBV genetic profile was observed in both old and recent isolates groups. However, most prominent diversity between viral isolates was obtained in the period from 1972 and 1989, showing the presence of a large number of variants in the state of Minas Gerais before the official approval of vaccination (1980).
publishDate 2010
dc.date.none.fl_str_mv 2010-10-28
2010
2011-04-10T11:11:11Z
2011-04-10T11:11:11Z
2018-05-28T11:11:11Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Revista Brasileira de Ciência Avícola, Campinas, v. 12, n. 2, p. 107-110, 2010.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/865545
10.1590/S1516-635X2010000200005
identifier_str_mv Revista Brasileira de Ciência Avícola, Campinas, v. 12, n. 2, p. 107-110, 2010.
10.1590/S1516-635X2010000200005
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/865545
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
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