Molecular studies of the brazilian infectious bronchitis virus isolates
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional do INMETRO |
Texto Completo: | http://hdl.handle.net/10926/791 |
Resumo: | ABSTRACT: Avian infectious bronchitis virus (IBV) isolates have been widely characterized by reverse transcription followed by polymerase chain reaction and DNA sequencing. In present study, these techniques were applied to three viral genomic regions comprising the complete and/or a partial S1 segment, S2 and nucleocapsid genes. DNA sequences from viral isolates obtained from 1972 to 1989 and from 2006 to 2008 were compared. High similarity (>90%) was observed among some of the genomic segments, including S1 hypervariable region, which could suggest a common origin or ancestry. DNA sequences from S2 and N protein genes obtained from different infected tissues of the same flock were analyzed, and a clear segregation between respiratory and intestinal tract was observed. Therefore, these data suggest cocirculation of more than one viral strain in the same flock. 57.1% of DNA sequences from the S1 complete segment samples, 53.3% from the S2 fragment and 62.5% from the partial N gene were found to be different from analyzed sequences from reference strains leading to the conclusion that parte of viral isolates included in this study may be considered region specific. Considering the simultaneous analysis of the three genes, a large IBV genetic profile was observed in both old and recent isolates groups. However, most prominent diversity between viral isolates was obtained in the period from 1972 and 1989, showing the presence of a large number of variants in the state of Minas Gerais before the official approval of vaccination (1980). |
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info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleMolecular studies of the brazilian infectious bronchitis virus isolates20102011-02-28T16:15:59Z2011-02-28T16:15:59ZABSTRACT: Avian infectious bronchitis virus (IBV) isolates have been widely characterized by reverse transcription followed by polymerase chain reaction and DNA sequencing. In present study, these techniques were applied to three viral genomic regions comprising the complete and/or a partial S1 segment, S2 and nucleocapsid genes. DNA sequences from viral isolates obtained from 1972 to 1989 and from 2006 to 2008 were compared. High similarity (>90%) was observed among some of the genomic segments, including S1 hypervariable region, which could suggest a common origin or ancestry. DNA sequences from S2 and N protein genes obtained from different infected tissues of the same flock were analyzed, and a clear segregation between respiratory and intestinal tract was observed. Therefore, these data suggest cocirculation of more than one viral strain in the same flock. 57.1% of DNA sequences from the S1 complete segment samples, 53.3% from the S2 fragment and 62.5% from the partial N gene were found to be different from analyzed sequences from reference strains leading to the conclusion that parte of viral isolates included in this study may be considered region specific. Considering the simultaneous analysis of the three genes, a large IBV genetic profile was observed in both old and recent isolates groups. However, most prominent diversity between viral isolates was obtained in the period from 1972 and 1989, showing the presence of a large number of variants in the state of Minas Gerais before the official approval of vaccination (1980).4 p. : il.Submitted by Regina Mello (mrmello@inmetro.gov.br) on 2011-02-09T13:16:42Z No. of bitstreams: 1 2010_AbreuMourãoSantos.pdf: 74538 bytes, checksum: 13351d05f4f85b5d2d584d171b8cc0f4 (MD5)Approved for entry into archive by Claudia Araujo(caraujo@inmetro.gov.br) on 2011-02-28T16:15:59Z (GMT) No. of bitstreams: 1 2010_AbreuMourãoSantos.pdf: 74538 bytes, checksum: 13351d05f4f85b5d2d584d171b8cc0f4 (MD5)Made available in DSpace on 2011-02-28T16:15:59Z (GMT). No. of bitstreams: 1 2010_AbreuMourãoSantos.pdf: 74538 bytes, checksum: 13351d05f4f85b5d2d584d171b8cc0f4 (MD5) Previous issue date: 2010enghttp://hdl.handle.net/10926/791DMD_hdl_10926/791ABREU, J. T. et al. Molecular studies of the brazilian infectious bronchitis virus isolates. Revista Brasileira de Ciência Avícola, v. 12 , n. 2, p. 107-110, 2010.Abreu, Josiane Tavares deMourão, Marina de MoraesSantos, Carlos Eduardo Fernandes dosVeloso, Caio Júlio MartinsResende, José Sérgio deFlatschart, Roberto BechtFolgueras-Flatschart, Aurea ValadaresNogueira Júnior, SérgioSantoro, Marcelo MatosMendes, Ana Cristina RibeiroFranco, Glória ReginaSilva, A.Campos, A. B.Fernandez, Sandrainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional do INMETROinstname:Instituto Nacional de Metrologia, Qualidade e Tecnologia (INMETRO)instacron:INMETROAbreu_2010.pdf.txthttp://xrepo01s.inmetro.gov.br/bitstream/10926/791/6/Abreu_2010.pdf.txttext/plain19422http://xrepo01s.inmetro.gov.br/bitstream/10926/791/6/Abreu_2010.pdf.txt632663480e5e5ae2242c0b4fd90bf355MD510926_791_6Abreu_2010.pdfhttp://xrepo01s.inmetro.gov.br/bitstream/10926/791/1/Abreu_2010.pdfapplication/pdf74538http://xrepo01s.inmetro.gov.br/bitstream/10926/791/1/Abreu_2010.pdf13351d05f4f85b5d2d584d171b8cc0f4MD510926_791_12024-06-10T15:24:30Zoai:xrepo01s.inmetro.gov.br:10926/791Repositório de Publicaçõeshttp://repositorios.inmetro.gov.br/oai/requestopendoar:2012-11-19T16:21:38Repositório Institucional do INMETRO - Instituto Nacional de Metrologia, Qualidade e Tecnologia (INMETRO)false |
dc.title.none.fl_str_mv |
Molecular studies of the brazilian infectious bronchitis virus isolates |
title |
Molecular studies of the brazilian infectious bronchitis virus isolates |
spellingShingle |
Molecular studies of the brazilian infectious bronchitis virus isolates Abreu, Josiane Tavares de |
title_short |
Molecular studies of the brazilian infectious bronchitis virus isolates |
title_full |
Molecular studies of the brazilian infectious bronchitis virus isolates |
title_fullStr |
Molecular studies of the brazilian infectious bronchitis virus isolates |
title_full_unstemmed |
Molecular studies of the brazilian infectious bronchitis virus isolates |
title_sort |
Molecular studies of the brazilian infectious bronchitis virus isolates |
author |
Abreu, Josiane Tavares de |
author_facet |
Abreu, Josiane Tavares de Mourão, Marina de Moraes Santos, Carlos Eduardo Fernandes dos Veloso, Caio Júlio Martins Resende, José Sérgio de Flatschart, Roberto Becht Folgueras-Flatschart, Aurea Valadares Nogueira Júnior, Sérgio Santoro, Marcelo Matos Mendes, Ana Cristina Ribeiro Franco, Glória Regina Silva, A. Campos, A. B. Fernandez, Sandra |
author_role |
author |
author2 |
Mourão, Marina de Moraes Santos, Carlos Eduardo Fernandes dos Veloso, Caio Júlio Martins Resende, José Sérgio de Flatschart, Roberto Becht Folgueras-Flatschart, Aurea Valadares Nogueira Júnior, Sérgio Santoro, Marcelo Matos Mendes, Ana Cristina Ribeiro Franco, Glória Regina Silva, A. Campos, A. B. Fernandez, Sandra |
author2_role |
author author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Abreu, Josiane Tavares de Mourão, Marina de Moraes Santos, Carlos Eduardo Fernandes dos Veloso, Caio Júlio Martins Resende, José Sérgio de Flatschart, Roberto Becht Folgueras-Flatschart, Aurea Valadares Nogueira Júnior, Sérgio Santoro, Marcelo Matos Mendes, Ana Cristina Ribeiro Franco, Glória Regina Silva, A. Campos, A. B. Fernandez, Sandra |
description |
ABSTRACT: Avian infectious bronchitis virus (IBV) isolates have been widely characterized by reverse transcription followed by polymerase chain reaction and DNA sequencing. In present study, these techniques were applied to three viral genomic regions comprising the complete and/or a partial S1 segment, S2 and nucleocapsid genes. DNA sequences from viral isolates obtained from 1972 to 1989 and from 2006 to 2008 were compared. High similarity (>90%) was observed among some of the genomic segments, including S1 hypervariable region, which could suggest a common origin or ancestry. DNA sequences from S2 and N protein genes obtained from different infected tissues of the same flock were analyzed, and a clear segregation between respiratory and intestinal tract was observed. Therefore, these data suggest cocirculation of more than one viral strain in the same flock. 57.1% of DNA sequences from the S1 complete segment samples, 53.3% from the S2 fragment and 62.5% from the partial N gene were found to be different from analyzed sequences from reference strains leading to the conclusion that parte of viral isolates included in this study may be considered region specific. Considering the simultaneous analysis of the three genes, a large IBV genetic profile was observed in both old and recent isolates groups. However, most prominent diversity between viral isolates was obtained in the period from 1972 and 1989, showing the presence of a large number of variants in the state of Minas Gerais before the official approval of vaccination (1980). |
publishDate |
2010 |
dc.date.issued.fl_str_mv |
2010 |
dc.date.available.fl_str_mv |
2011-02-28T16:15:59Z |
dc.date.accessioned.fl_str_mv |
2011-02-28T16:15:59Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10926/791 DMD_hdl_10926/791 |
dc.identifier.citation.fl_str_mv |
ABREU, J. T. et al. Molecular studies of the brazilian infectious bronchitis virus isolates. Revista Brasileira de Ciência Avícola, v. 12 , n. 2, p. 107-110, 2010. |
url |
http://hdl.handle.net/10926/791 |
identifier_str_mv |
DMD_hdl_10926/791 ABREU, J. T. et al. Molecular studies of the brazilian infectious bronchitis virus isolates. Revista Brasileira de Ciência Avícola, v. 12 , n. 2, p. 107-110, 2010. |
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eng |
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text/plain application/pdf |
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