Rapid method for simultaneous determination of CIS-9, TRANS-11 and TRANS-10, CIS-12 conjugated linoleic acid isomers in milk by GC-FID.
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1156165 |
Resumo: | Ruminant milk is the lead source of conjugated linoleic acid (CLA) in the human diet, with cis-9, trans-11 CLA being the major among all. Small amounts of trans-10, cis-12 CLA are also found in synthetic supplements. Since both isomers are biologically active with potential health benefits, there is great interest in quantifying them for quality control routines. An alternative method for the analysis of the aforementioned CLAs by fast gas chromatography (GC) is discussed in the present study. The fatty acid methyl ester mixture obtained by alkaline catalysis was injected into a GC equipped with a flame ionization detector (FID) and fitted with an ionic liquid SLB-IL111 chromatographic column (15 m × 0.10 mm × 0.08 µm). Separation was achieved in less than 5 min using a 168 °C isotherm run. Both CLA isomers were quantified by using of single point standard addition statistical approach. Results were contested to those obtained using the recommended 100 m long CP-Sil88 capillary column and none evidence of significant differences was found within 95% confidence interval. Therefore, the proposed method could be valuable to focused regulatory routines of large numbers of samples with greater analytical frequency. |
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Rapid method for simultaneous determination of CIS-9, TRANS-11 and TRANS-10, CIS-12 conjugated linoleic acid isomers in milk by GC-FID.Fast GCCLASLB IL111MilkFatty acidsRuminant milk is the lead source of conjugated linoleic acid (CLA) in the human diet, with cis-9, trans-11 CLA being the major among all. Small amounts of trans-10, cis-12 CLA are also found in synthetic supplements. Since both isomers are biologically active with potential health benefits, there is great interest in quantifying them for quality control routines. An alternative method for the analysis of the aforementioned CLAs by fast gas chromatography (GC) is discussed in the present study. The fatty acid methyl ester mixture obtained by alkaline catalysis was injected into a GC equipped with a flame ionization detector (FID) and fitted with an ionic liquid SLB-IL111 chromatographic column (15 m × 0.10 mm × 0.08 µm). Separation was achieved in less than 5 min using a 168 °C isotherm run. Both CLA isomers were quantified by using of single point standard addition statistical approach. Results were contested to those obtained using the recommended 100 m long CP-Sil88 capillary column and none evidence of significant differences was found within 95% confidence interval. Therefore, the proposed method could be valuable to focused regulatory routines of large numbers of samples with greater analytical frequency.BRENDA LEE SIMAS PORTO, Universidade Federal de Juiz de ForaISAURA DANIELE LEITE FARIA, Universidade Federal de Juiz de ForaJÉSSICA CORDEIRO QUEIROZ DE SOUZA, Universidade Federal de Juiz de ForaOLÍVIA BRITO DE OLIVEIRA MOREIRA, Universidade Federal de Juiz de ForaMARCO ANTONIO SUNDFELD DA GAMA, CPPSEMARCONE AUGUSTO LEAL DE OLIVEIRA, Universidade Estadual de Campinas.PORTO, B. L. S.FARIA, I. D. L.SOUZA, J. C. Q. DEMOREIRA, O. B. DE O.GAMA, M. A. S. daOLIVEIRA, M. A. L. DE2023-08-28T16:23:41Z2023-08-28T16:23:41Z2023-08-282023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleQuímica Nova, v. 7, p. 743-746, 2023.http://www.alice.cnptia.embrapa.br/alice/handle/doc/115616510.21577/0100-4042.20230026enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2023-08-28T16:23:41Zoai:www.alice.cnptia.embrapa.br:doc/1156165Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542023-08-28T16:23:41falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542023-08-28T16:23:41Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Rapid method for simultaneous determination of CIS-9, TRANS-11 and TRANS-10, CIS-12 conjugated linoleic acid isomers in milk by GC-FID. |
title |
Rapid method for simultaneous determination of CIS-9, TRANS-11 and TRANS-10, CIS-12 conjugated linoleic acid isomers in milk by GC-FID. |
spellingShingle |
Rapid method for simultaneous determination of CIS-9, TRANS-11 and TRANS-10, CIS-12 conjugated linoleic acid isomers in milk by GC-FID. PORTO, B. L. S. Fast GC CLA SLB IL111 Milk Fatty acids |
title_short |
Rapid method for simultaneous determination of CIS-9, TRANS-11 and TRANS-10, CIS-12 conjugated linoleic acid isomers in milk by GC-FID. |
title_full |
Rapid method for simultaneous determination of CIS-9, TRANS-11 and TRANS-10, CIS-12 conjugated linoleic acid isomers in milk by GC-FID. |
title_fullStr |
Rapid method for simultaneous determination of CIS-9, TRANS-11 and TRANS-10, CIS-12 conjugated linoleic acid isomers in milk by GC-FID. |
title_full_unstemmed |
Rapid method for simultaneous determination of CIS-9, TRANS-11 and TRANS-10, CIS-12 conjugated linoleic acid isomers in milk by GC-FID. |
title_sort |
Rapid method for simultaneous determination of CIS-9, TRANS-11 and TRANS-10, CIS-12 conjugated linoleic acid isomers in milk by GC-FID. |
author |
PORTO, B. L. S. |
author_facet |
PORTO, B. L. S. FARIA, I. D. L. SOUZA, J. C. Q. DE MOREIRA, O. B. DE O. GAMA, M. A. S. da OLIVEIRA, M. A. L. DE |
author_role |
author |
author2 |
FARIA, I. D. L. SOUZA, J. C. Q. DE MOREIRA, O. B. DE O. GAMA, M. A. S. da OLIVEIRA, M. A. L. DE |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
BRENDA LEE SIMAS PORTO, Universidade Federal de Juiz de Fora ISAURA DANIELE LEITE FARIA, Universidade Federal de Juiz de Fora JÉSSICA CORDEIRO QUEIROZ DE SOUZA, Universidade Federal de Juiz de Fora OLÍVIA BRITO DE OLIVEIRA MOREIRA, Universidade Federal de Juiz de Fora MARCO ANTONIO SUNDFELD DA GAMA, CPPSE MARCONE AUGUSTO LEAL DE OLIVEIRA, Universidade Estadual de Campinas. |
dc.contributor.author.fl_str_mv |
PORTO, B. L. S. FARIA, I. D. L. SOUZA, J. C. Q. DE MOREIRA, O. B. DE O. GAMA, M. A. S. da OLIVEIRA, M. A. L. DE |
dc.subject.por.fl_str_mv |
Fast GC CLA SLB IL111 Milk Fatty acids |
topic |
Fast GC CLA SLB IL111 Milk Fatty acids |
description |
Ruminant milk is the lead source of conjugated linoleic acid (CLA) in the human diet, with cis-9, trans-11 CLA being the major among all. Small amounts of trans-10, cis-12 CLA are also found in synthetic supplements. Since both isomers are biologically active with potential health benefits, there is great interest in quantifying them for quality control routines. An alternative method for the analysis of the aforementioned CLAs by fast gas chromatography (GC) is discussed in the present study. The fatty acid methyl ester mixture obtained by alkaline catalysis was injected into a GC equipped with a flame ionization detector (FID) and fitted with an ionic liquid SLB-IL111 chromatographic column (15 m × 0.10 mm × 0.08 µm). Separation was achieved in less than 5 min using a 168 °C isotherm run. Both CLA isomers were quantified by using of single point standard addition statistical approach. Results were contested to those obtained using the recommended 100 m long CP-Sil88 capillary column and none evidence of significant differences was found within 95% confidence interval. Therefore, the proposed method could be valuable to focused regulatory routines of large numbers of samples with greater analytical frequency. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-08-28T16:23:41Z 2023-08-28T16:23:41Z 2023-08-28 2023 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Química Nova, v. 7, p. 743-746, 2023. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1156165 10.21577/0100-4042.20230026 |
identifier_str_mv |
Química Nova, v. 7, p. 743-746, 2023. 10.21577/0100-4042.20230026 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1156165 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503549021847552 |