Altered expression of the caffeine synthase gene in a naturally caffeine-free mutant of Coffea arabica.
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/880373 |
Resumo: | In this work, we studied the biosynthesis of caffeine by examining the expression of genes involved in this biosynthetic pathway in coffee fruits containing normal or low levels of this substance. The amplification of gene-specific transcripts during fruit development revealed that low-caffeine fruits had a lower expression of the theobromine synthase and caffeine synthase genes and also contained an extra transcript of the caffeine synthase gene. This extra transcript contained only part of exon 1 and all of exon 3. The sequence of the mutant caffeine synthase gene revealed the substitution of isoleucine for valine in the enzyme active site that probably interfered with enzymatic activity. These findings indicate that the absence of caffeine in these mutants probably resulted from a combination of transcriptional regulation and the presence of mutations in the caffeine synthase amino acid sequence. |
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Altered expression of the caffeine synthase gene in a naturally caffeine-free mutant of Coffea arabica.Naturally decaffeinatedCoffeeCup qualityDifferential expressionIn this work, we studied the biosynthesis of caffeine by examining the expression of genes involved in this biosynthetic pathway in coffee fruits containing normal or low levels of this substance. The amplification of gene-specific transcripts during fruit development revealed that low-caffeine fruits had a lower expression of the theobromine synthase and caffeine synthase genes and also contained an extra transcript of the caffeine synthase gene. This extra transcript contained only part of exon 1 and all of exon 3. The sequence of the mutant caffeine synthase gene revealed the substitution of isoleucine for valine in the enzyme active site that probably interfered with enzymatic activity. These findings indicate that the absence of caffeine in these mutants probably resulted from a combination of transcriptional regulation and the presence of mutations in the caffeine synthase amino acid sequence.MIRIAN PEREZ MALUF, SAPC; CARLA CRISTINA DA SILVA, INSTITUTO AGRONÔMICO, CENTRO DE CAFE 'ALCIDES CARVALHO'; MICHELLE DE PAULA ABREU DE OLIVEIRA, INSTITUTO AGRONÔMICO, CENTRO DE CAFE 'ALCIDES CARVALHO'; ALINE GOMES TAVARES, INSTITUTO AGRONÔMICO, CENTRO DE CAFE 'ALCIDES CARVALHO'; MARIA BERNADETE SILVAROLLA, INSTITUTO AGRONÔMICO, CENTRO DE CAFE 'ALCIDES CARVALHO'; OLIVEIRO GUERREIRO FILHO, INSTITUTO AGRONÔMICO, CENTRO DE CAFE 'ALCIDES CARVALHO'.MALUF, M. P.SILVA, C. C. daOLIVEIRA, M. de P. A. deTAVARES, A. G.SILVAROLLA, M. B.GUERREIRO FILHO, O.2011-04-09T22:27:49Z2011-04-09T22:27:49Z2011-03-1020092011-04-10T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleGenetics and Molecular Biology, v. 34, n. 2, p. 802-810, 2009.http://www.alice.cnptia.embrapa.br/alice/handle/doc/880373enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-15T22:28:49Zoai:www.alice.cnptia.embrapa.br:doc/880373Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-15T22:28:49falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-15T22:28:49Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Altered expression of the caffeine synthase gene in a naturally caffeine-free mutant of Coffea arabica. |
title |
Altered expression of the caffeine synthase gene in a naturally caffeine-free mutant of Coffea arabica. |
spellingShingle |
Altered expression of the caffeine synthase gene in a naturally caffeine-free mutant of Coffea arabica. MALUF, M. P. Naturally decaffeinated Coffee Cup quality Differential expression |
title_short |
Altered expression of the caffeine synthase gene in a naturally caffeine-free mutant of Coffea arabica. |
title_full |
Altered expression of the caffeine synthase gene in a naturally caffeine-free mutant of Coffea arabica. |
title_fullStr |
Altered expression of the caffeine synthase gene in a naturally caffeine-free mutant of Coffea arabica. |
title_full_unstemmed |
Altered expression of the caffeine synthase gene in a naturally caffeine-free mutant of Coffea arabica. |
title_sort |
Altered expression of the caffeine synthase gene in a naturally caffeine-free mutant of Coffea arabica. |
author |
MALUF, M. P. |
author_facet |
MALUF, M. P. SILVA, C. C. da OLIVEIRA, M. de P. A. de TAVARES, A. G. SILVAROLLA, M. B. GUERREIRO FILHO, O. |
author_role |
author |
author2 |
SILVA, C. C. da OLIVEIRA, M. de P. A. de TAVARES, A. G. SILVAROLLA, M. B. GUERREIRO FILHO, O. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
MIRIAN PEREZ MALUF, SAPC; CARLA CRISTINA DA SILVA, INSTITUTO AGRONÔMICO, CENTRO DE CAFE 'ALCIDES CARVALHO'; MICHELLE DE PAULA ABREU DE OLIVEIRA, INSTITUTO AGRONÔMICO, CENTRO DE CAFE 'ALCIDES CARVALHO'; ALINE GOMES TAVARES, INSTITUTO AGRONÔMICO, CENTRO DE CAFE 'ALCIDES CARVALHO'; MARIA BERNADETE SILVAROLLA, INSTITUTO AGRONÔMICO, CENTRO DE CAFE 'ALCIDES CARVALHO'; OLIVEIRO GUERREIRO FILHO, INSTITUTO AGRONÔMICO, CENTRO DE CAFE 'ALCIDES CARVALHO'. |
dc.contributor.author.fl_str_mv |
MALUF, M. P. SILVA, C. C. da OLIVEIRA, M. de P. A. de TAVARES, A. G. SILVAROLLA, M. B. GUERREIRO FILHO, O. |
dc.subject.por.fl_str_mv |
Naturally decaffeinated Coffee Cup quality Differential expression |
topic |
Naturally decaffeinated Coffee Cup quality Differential expression |
description |
In this work, we studied the biosynthesis of caffeine by examining the expression of genes involved in this biosynthetic pathway in coffee fruits containing normal or low levels of this substance. The amplification of gene-specific transcripts during fruit development revealed that low-caffeine fruits had a lower expression of the theobromine synthase and caffeine synthase genes and also contained an extra transcript of the caffeine synthase gene. This extra transcript contained only part of exon 1 and all of exon 3. The sequence of the mutant caffeine synthase gene revealed the substitution of isoleucine for valine in the enzyme active site that probably interfered with enzymatic activity. These findings indicate that the absence of caffeine in these mutants probably resulted from a combination of transcriptional regulation and the presence of mutations in the caffeine synthase amino acid sequence. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009 2011-04-09T22:27:49Z 2011-04-09T22:27:49Z 2011-03-10 2011-04-10T11:11:11Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Genetics and Molecular Biology, v. 34, n. 2, p. 802-810, 2009. http://www.alice.cnptia.embrapa.br/alice/handle/doc/880373 |
identifier_str_mv |
Genetics and Molecular Biology, v. 34, n. 2, p. 802-810, 2009. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/880373 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503340772556800 |