Salvinia auriculata: chemical profle and biological activity against Staphylococcus aureus isolated from bovine mastitis.
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1134757 https://doi.org/10.1007/s42770-021-00595-z |
Resumo: | The aquatic plant Salvinia auriculata has been shown to possess promising properties for the treatment of Staphylococcus aureus bovine mastitis. The disease afects cattle health and compromises dairy cattle productivity, resulting in reduced milk production and higher mortality rates. The aim of this study was to evaluate the antimicrobial activity, antibioflm activity, and toxicity of S. auriculata root extracts using bovine mammary epithelial cells (MAC-T); determine the chemical composition of the most active extract; and develop an S. auriculata antiseptic solution for pre- and post-milking teat disinfection. Plants were collected during the four seasons of the year. The most active hexane extract was subjected to bioguided fractionation, which resulted in the isolation of six known compounds, stigmast-22-ene-3,6-dione, stigmasterol, friedelinol, ?-sitosterol, octadecyl alcohol, and octadecanoic acid. The antimicrobial and antibioflm activities of the most active extract and isolated compounds were determined against nine S. aureus strains isolated from cows with mastitis. The efcacy of the S. auriculata teat dip formulation was tested using an excised teat model (ex vivo), and promising results were obtained. The S. auriculata extract formulation proved to be as efective as commercial antimicrobials in reducing log counts in excised teats. |
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Salvinia auriculata: chemical profle and biological activity against Staphylococcus aureus isolated from bovine mastitis.Atividade antimicrobianaFormulação anti-sépticaFitosterolAtividade biológicaPerfil químicoMastiteBovinoBactériaThe aquatic plant Salvinia auriculata has been shown to possess promising properties for the treatment of Staphylococcus aureus bovine mastitis. The disease afects cattle health and compromises dairy cattle productivity, resulting in reduced milk production and higher mortality rates. The aim of this study was to evaluate the antimicrobial activity, antibioflm activity, and toxicity of S. auriculata root extracts using bovine mammary epithelial cells (MAC-T); determine the chemical composition of the most active extract; and develop an S. auriculata antiseptic solution for pre- and post-milking teat disinfection. Plants were collected during the four seasons of the year. The most active hexane extract was subjected to bioguided fractionation, which resulted in the isolation of six known compounds, stigmast-22-ene-3,6-dione, stigmasterol, friedelinol, ?-sitosterol, octadecyl alcohol, and octadecanoic acid. The antimicrobial and antibioflm activities of the most active extract and isolated compounds were determined against nine S. aureus strains isolated from cows with mastitis. The efcacy of the S. auriculata teat dip formulation was tested using an excised teat model (ex vivo), and promising results were obtained. The S. auriculata extract formulation proved to be as efective as commercial antimicrobials in reducing log counts in excised teats.GISLAINE APARECIDA PURGATO, Universidade Federal de Viçosa; SÂMIA LIMA, Universidade Federal de Viçosa; JEFERSON VICTOR PAULA BARROS BAETA, Fundação Ezequiel Dias; VIRGÍNIA RAMOS PIZZIOLO, Universidade Federal de Viçosa; GUILHERME NUNES DE SOUZA, CNPGL; GASPAR DIAZ-MUÑOZ, Universidade Federal de Minas Gerais; MARISA ALVES NOGUEIRA DIAZ, Universidade Federal de Viçosa.PURGATO, G. A.LIMA, S.BAETA, J. V. P. B.PIZZIOLO, V. R.SOUZA, G. N. deDIAZ-MUÑOZ, G.DIAZ, M. A. N.2021-09-24T14:00:43Z2021-09-24T14:00:43Z2021-09-242021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleBrazilian Journal of Microbiology, v. 52, n. 4, p. 2401-2411, 2021.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1134757https://doi.org/10.1007/s42770-021-00595-zenginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2021-09-24T14:00:52Zoai:www.alice.cnptia.embrapa.br:doc/1134757Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542021-09-24T14:00:52falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542021-09-24T14:00:52Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Salvinia auriculata: chemical profle and biological activity against Staphylococcus aureus isolated from bovine mastitis. |
title |
Salvinia auriculata: chemical profle and biological activity against Staphylococcus aureus isolated from bovine mastitis. |
spellingShingle |
Salvinia auriculata: chemical profle and biological activity against Staphylococcus aureus isolated from bovine mastitis. PURGATO, G. A. Atividade antimicrobiana Formulação anti-séptica Fitosterol Atividade biológica Perfil químico Mastite Bovino Bactéria |
title_short |
Salvinia auriculata: chemical profle and biological activity against Staphylococcus aureus isolated from bovine mastitis. |
title_full |
Salvinia auriculata: chemical profle and biological activity against Staphylococcus aureus isolated from bovine mastitis. |
title_fullStr |
Salvinia auriculata: chemical profle and biological activity against Staphylococcus aureus isolated from bovine mastitis. |
title_full_unstemmed |
Salvinia auriculata: chemical profle and biological activity against Staphylococcus aureus isolated from bovine mastitis. |
title_sort |
Salvinia auriculata: chemical profle and biological activity against Staphylococcus aureus isolated from bovine mastitis. |
author |
PURGATO, G. A. |
author_facet |
PURGATO, G. A. LIMA, S. BAETA, J. V. P. B. PIZZIOLO, V. R. SOUZA, G. N. de DIAZ-MUÑOZ, G. DIAZ, M. A. N. |
author_role |
author |
author2 |
LIMA, S. BAETA, J. V. P. B. PIZZIOLO, V. R. SOUZA, G. N. de DIAZ-MUÑOZ, G. DIAZ, M. A. N. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
GISLAINE APARECIDA PURGATO, Universidade Federal de Viçosa; SÂMIA LIMA, Universidade Federal de Viçosa; JEFERSON VICTOR PAULA BARROS BAETA, Fundação Ezequiel Dias; VIRGÍNIA RAMOS PIZZIOLO, Universidade Federal de Viçosa; GUILHERME NUNES DE SOUZA, CNPGL; GASPAR DIAZ-MUÑOZ, Universidade Federal de Minas Gerais; MARISA ALVES NOGUEIRA DIAZ, Universidade Federal de Viçosa. |
dc.contributor.author.fl_str_mv |
PURGATO, G. A. LIMA, S. BAETA, J. V. P. B. PIZZIOLO, V. R. SOUZA, G. N. de DIAZ-MUÑOZ, G. DIAZ, M. A. N. |
dc.subject.por.fl_str_mv |
Atividade antimicrobiana Formulação anti-séptica Fitosterol Atividade biológica Perfil químico Mastite Bovino Bactéria |
topic |
Atividade antimicrobiana Formulação anti-séptica Fitosterol Atividade biológica Perfil químico Mastite Bovino Bactéria |
description |
The aquatic plant Salvinia auriculata has been shown to possess promising properties for the treatment of Staphylococcus aureus bovine mastitis. The disease afects cattle health and compromises dairy cattle productivity, resulting in reduced milk production and higher mortality rates. The aim of this study was to evaluate the antimicrobial activity, antibioflm activity, and toxicity of S. auriculata root extracts using bovine mammary epithelial cells (MAC-T); determine the chemical composition of the most active extract; and develop an S. auriculata antiseptic solution for pre- and post-milking teat disinfection. Plants were collected during the four seasons of the year. The most active hexane extract was subjected to bioguided fractionation, which resulted in the isolation of six known compounds, stigmast-22-ene-3,6-dione, stigmasterol, friedelinol, ?-sitosterol, octadecyl alcohol, and octadecanoic acid. The antimicrobial and antibioflm activities of the most active extract and isolated compounds were determined against nine S. aureus strains isolated from cows with mastitis. The efcacy of the S. auriculata teat dip formulation was tested using an excised teat model (ex vivo), and promising results were obtained. The S. auriculata extract formulation proved to be as efective as commercial antimicrobials in reducing log counts in excised teats. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-09-24T14:00:43Z 2021-09-24T14:00:43Z 2021-09-24 2021 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Brazilian Journal of Microbiology, v. 52, n. 4, p. 2401-2411, 2021. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1134757 https://doi.org/10.1007/s42770-021-00595-z |
identifier_str_mv |
Brazilian Journal of Microbiology, v. 52, n. 4, p. 2401-2411, 2021. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1134757 https://doi.org/10.1007/s42770-021-00595-z |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
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Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
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EMBRAPA |
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EMBRAPA |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503509855436800 |