DNA methylation may affect beef tenderness through signal transduction in Bos indicus.
Autor(a) principal: | |
---|---|
Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1144144 https://doi.org/10.1186/s13072-022-00449-4 |
Resumo: | Abstract. Background:Beef tenderness is a complex trait of economic importance for the beef industry. Understanding the epigenetic mechanisms underlying this trait may help improve the accuracy of breeding programs. However, little is known about epigenetic effects on Bos taurus muscle and their implications in tenderness, and no studies have been conducted in Bos indicus.Results:Comparing methylation profile of Bos indicus skeletal muscle with contrasting beef tenderness at 14 days after slaughter, we identified differentially methylated cytosines and regions associated with this trait. Interestingly, muscle that became tender beef had higher levels of hypermethylation compared to the tough group. Enrichment analysis of predicted target genes suggested that differences in methylation between tender and tough beef may affect signal transduction pathways, among which G protein signaling was a key pathway. In addition, different meth-ylation levels were found associated with expression levels of GNAS, PDE4B, EPCAM and EBF3 genes. The differentially methylated elements correlated with EBF3 and GNAS genes overlapped CpG islands and regulatory elements. GNAS, a complex imprinted gene, has a key role on G protein signaling pathways. Moreover, both G protein signaling pathway and the EBF3 gene regulate muscle homeostasis, relaxation, and muscle cell-specificity.Conclusions:We present differentially methylated loci that may be of interest to decipher the epigenetic mecha-nisms affecting tenderness. Supported by the previous knowledge about regulatory elements and gene function, the methylation data suggests EBF3 and GNAS as potential candidate genes and G protein signaling as potential candi-date pathway associated with beef tenderness via methylation. |
id |
EMBR_fd238690d45d549b58281826b552d116 |
---|---|
oai_identifier_str |
oai:www.alice.cnptia.embrapa.br:doc/1144144 |
network_acronym_str |
EMBR |
network_name_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository_id_str |
2154 |
spelling |
DNA methylation may affect beef tenderness through signal transduction in Bos indicus.NeloreEpigenomaMúsculo de neloreRRBSGNASEBF3Força de cisalhamentoShear forceEpigenomeMuscleMetilaçãoBos TaurusBos IndicusMúsculoCattleMethylationDNA methylationAbstract. Background:Beef tenderness is a complex trait of economic importance for the beef industry. Understanding the epigenetic mechanisms underlying this trait may help improve the accuracy of breeding programs. However, little is known about epigenetic effects on Bos taurus muscle and their implications in tenderness, and no studies have been conducted in Bos indicus.Results:Comparing methylation profile of Bos indicus skeletal muscle with contrasting beef tenderness at 14 days after slaughter, we identified differentially methylated cytosines and regions associated with this trait. Interestingly, muscle that became tender beef had higher levels of hypermethylation compared to the tough group. Enrichment analysis of predicted target genes suggested that differences in methylation between tender and tough beef may affect signal transduction pathways, among which G protein signaling was a key pathway. In addition, different meth-ylation levels were found associated with expression levels of GNAS, PDE4B, EPCAM and EBF3 genes. The differentially methylated elements correlated with EBF3 and GNAS genes overlapped CpG islands and regulatory elements. GNAS, a complex imprinted gene, has a key role on G protein signaling pathways. Moreover, both G protein signaling pathway and the EBF3 gene regulate muscle homeostasis, relaxation, and muscle cell-specificity.Conclusions:We present differentially methylated loci that may be of interest to decipher the epigenetic mecha-nisms affecting tenderness. Supported by the previous knowledge about regulatory elements and gene function, the methylation data suggests EBF3 and GNAS as potential candidate genes and G protein signaling as potential candi-date pathway associated with beef tenderness via methylation.Article number: 15.MARCELA MARIA DE SOUZA, IOWA STATE UNIVERSITY; SIMONE CRISTINA MEO NICIURA, CPPSE; MARINA IBELLI PEREIRA ROCHA, UFSCAR; ZHANGYUAN PAN, UNIVERSITY OF CALIFORNIA; HUAIJUN ZHOU, UNIVERSITY OF CALIFORNIA; JENNIFER JESSICA BRUSCADIN, UFSCAR; WELLISON JARLES DA SILVA DINIZ, AUBURN UNIVERSITY; JULIANA AFONSO; PRISCILA SILVA NEUBERN DE OLIVEIRA, UFSCAR; GERSON B. MOURÃO, ESALQ/USP; ADHEMAR ZERLOTINI NETO, CNPTIA; LUIZ LEHMANN COUTINHO, ESALQ/USP; JAMES E. KOLTES, IOWA STATE UNIVERSITY; LUCIANA CORREIA DE ALMEIDA REGITANO, CPPSE.SOUZA, M. M. deNICIURA, S. C. M.ROCHA, M. I. P.PAN, Z.ZHOU, H.BRUSCADIN, J. J.DINIZ, W. J. da S.AFONSO, J.OLIVEIRA, P. S. N. deMOURÃO, G. B.ZERLOTINI NETO, A.COUTINHO, L. L.KOLTES, J. E.REGITANO, L. C. de A.2022-06-20T13:20:10Z2022-06-20T13:20:10Z2022-06-202022info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleEpigenetics & Chromatin, v. 15, n. 1, p. 1-16, 2022.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1144144https://doi.org/10.1186/s13072-022-00449-4enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2022-06-20T13:20:20Zoai:www.alice.cnptia.embrapa.br:doc/1144144Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542022-06-20T13:20:20falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542022-06-20T13:20:20Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
DNA methylation may affect beef tenderness through signal transduction in Bos indicus. |
title |
DNA methylation may affect beef tenderness through signal transduction in Bos indicus. |
spellingShingle |
DNA methylation may affect beef tenderness through signal transduction in Bos indicus. SOUZA, M. M. de Nelore Epigenoma Músculo de nelore RRBS GNAS EBF3 Força de cisalhamento Shear force Epigenome Muscle Metilação Bos Taurus Bos Indicus Músculo Cattle Methylation DNA methylation |
title_short |
DNA methylation may affect beef tenderness through signal transduction in Bos indicus. |
title_full |
DNA methylation may affect beef tenderness through signal transduction in Bos indicus. |
title_fullStr |
DNA methylation may affect beef tenderness through signal transduction in Bos indicus. |
title_full_unstemmed |
DNA methylation may affect beef tenderness through signal transduction in Bos indicus. |
title_sort |
DNA methylation may affect beef tenderness through signal transduction in Bos indicus. |
author |
SOUZA, M. M. de |
author_facet |
SOUZA, M. M. de NICIURA, S. C. M. ROCHA, M. I. P. PAN, Z. ZHOU, H. BRUSCADIN, J. J. DINIZ, W. J. da S. AFONSO, J. OLIVEIRA, P. S. N. de MOURÃO, G. B. ZERLOTINI NETO, A. COUTINHO, L. L. KOLTES, J. E. REGITANO, L. C. de A. |
author_role |
author |
author2 |
NICIURA, S. C. M. ROCHA, M. I. P. PAN, Z. ZHOU, H. BRUSCADIN, J. J. DINIZ, W. J. da S. AFONSO, J. OLIVEIRA, P. S. N. de MOURÃO, G. B. ZERLOTINI NETO, A. COUTINHO, L. L. KOLTES, J. E. REGITANO, L. C. de A. |
author2_role |
author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
MARCELA MARIA DE SOUZA, IOWA STATE UNIVERSITY; SIMONE CRISTINA MEO NICIURA, CPPSE; MARINA IBELLI PEREIRA ROCHA, UFSCAR; ZHANGYUAN PAN, UNIVERSITY OF CALIFORNIA; HUAIJUN ZHOU, UNIVERSITY OF CALIFORNIA; JENNIFER JESSICA BRUSCADIN, UFSCAR; WELLISON JARLES DA SILVA DINIZ, AUBURN UNIVERSITY; JULIANA AFONSO; PRISCILA SILVA NEUBERN DE OLIVEIRA, UFSCAR; GERSON B. MOURÃO, ESALQ/USP; ADHEMAR ZERLOTINI NETO, CNPTIA; LUIZ LEHMANN COUTINHO, ESALQ/USP; JAMES E. KOLTES, IOWA STATE UNIVERSITY; LUCIANA CORREIA DE ALMEIDA REGITANO, CPPSE. |
dc.contributor.author.fl_str_mv |
SOUZA, M. M. de NICIURA, S. C. M. ROCHA, M. I. P. PAN, Z. ZHOU, H. BRUSCADIN, J. J. DINIZ, W. J. da S. AFONSO, J. OLIVEIRA, P. S. N. de MOURÃO, G. B. ZERLOTINI NETO, A. COUTINHO, L. L. KOLTES, J. E. REGITANO, L. C. de A. |
dc.subject.por.fl_str_mv |
Nelore Epigenoma Músculo de nelore RRBS GNAS EBF3 Força de cisalhamento Shear force Epigenome Muscle Metilação Bos Taurus Bos Indicus Músculo Cattle Methylation DNA methylation |
topic |
Nelore Epigenoma Músculo de nelore RRBS GNAS EBF3 Força de cisalhamento Shear force Epigenome Muscle Metilação Bos Taurus Bos Indicus Músculo Cattle Methylation DNA methylation |
description |
Abstract. Background:Beef tenderness is a complex trait of economic importance for the beef industry. Understanding the epigenetic mechanisms underlying this trait may help improve the accuracy of breeding programs. However, little is known about epigenetic effects on Bos taurus muscle and their implications in tenderness, and no studies have been conducted in Bos indicus.Results:Comparing methylation profile of Bos indicus skeletal muscle with contrasting beef tenderness at 14 days after slaughter, we identified differentially methylated cytosines and regions associated with this trait. Interestingly, muscle that became tender beef had higher levels of hypermethylation compared to the tough group. Enrichment analysis of predicted target genes suggested that differences in methylation between tender and tough beef may affect signal transduction pathways, among which G protein signaling was a key pathway. In addition, different meth-ylation levels were found associated with expression levels of GNAS, PDE4B, EPCAM and EBF3 genes. The differentially methylated elements correlated with EBF3 and GNAS genes overlapped CpG islands and regulatory elements. GNAS, a complex imprinted gene, has a key role on G protein signaling pathways. Moreover, both G protein signaling pathway and the EBF3 gene regulate muscle homeostasis, relaxation, and muscle cell-specificity.Conclusions:We present differentially methylated loci that may be of interest to decipher the epigenetic mecha-nisms affecting tenderness. Supported by the previous knowledge about regulatory elements and gene function, the methylation data suggests EBF3 and GNAS as potential candidate genes and G protein signaling as potential candi-date pathway associated with beef tenderness via methylation. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-06-20T13:20:10Z 2022-06-20T13:20:10Z 2022-06-20 2022 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Epigenetics & Chromatin, v. 15, n. 1, p. 1-16, 2022. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1144144 https://doi.org/10.1186/s13072-022-00449-4 |
identifier_str_mv |
Epigenetics & Chromatin, v. 15, n. 1, p. 1-16, 2022. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1144144 https://doi.org/10.1186/s13072-022-00449-4 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
_version_ |
1794503524911939584 |