Is the treatment of skin wounds with therapeutic ultrasound effective? Analysis and proposal of an experimental protocol
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por eng |
Título da fonte: | Scire Salutis |
Texto Completo: | https://sustenere.inf.br/index.php/sciresalutis/article/view/8080 |
Resumo: | In Brazil, wounds are a serious public health problem, due to the large number of patients with changes in skin integrity, although the records of these treatments are scarce. In an attempt to minimize tissue healing time, several animal studies have been presenting the use of therapeutic ultrasound as a coadjuvant factor. However, there is no standardization of doses for use of this therapy in cutaneous wounds. Objective: To propose a protocol to evaluate efficacy in the treatment of experimental cutaneous wounds by means of pulsed therapeutic ultrasound of 3 MHz through 1.0W/cm2, 1.5W/cm2, 2.0W/cm2. This is an experimental and randomized study. Sixty male Wistar rats were used. After anesthetized, the animals underwent surgery to remove a fragment of the skin in the region of the animal's back with the use of dermatological punch in the 8. The animals were randomly randomized in 4 different groups being: control group; GA (1.0W/cm2); GB (1.5w / cm2) and GC (2.0w / cm2). The device was fixed to the other parameters: 3MHz, pulsed with 20% pulse with, 100Hz, for 2 minutes. The treatment was performed daily. The control group was manipulated with the device switched off. Each group was evaluated in 3 different time frames: 3rd, 7th and 15th postoperative day. Biometric, morphometric and stereological evaluations were performed. For the statistic, the PRISM 6.0 software was used with the TWO-WAY ANOVA test with BONFERRONI post-test considering p <0.05. In the evaluation of the variables for retraction of the wound area, retraction percentage as well as the daily retraction rate presented p> 0.05 when compared to the control group versus the experimental group. Regarding the thickness of the epithelium and keratin, there was no significant difference between the control group and the treated groups, however, groups irradiated with higher frequencies had worse results in the healing process. In this experimental model, the protocol for the use of therapeutic ultrasound interferes negatively in the process of wound healing when used in doses starting at 1.5W / cm2. |
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Is the treatment of skin wounds with therapeutic ultrasound effective? Analysis and proposal of an experimental protocolO tratamento de feridas cutâneas com ultrassom terapêutico é eficaz? Análise e proposta de um protocolo experimentalUltrassom terapêuticoFeridas cutâneasCicatrizaçãoTherapeutic ultrasoundCutaneous wounds HealingIn Brazil, wounds are a serious public health problem, due to the large number of patients with changes in skin integrity, although the records of these treatments are scarce. In an attempt to minimize tissue healing time, several animal studies have been presenting the use of therapeutic ultrasound as a coadjuvant factor. However, there is no standardization of doses for use of this therapy in cutaneous wounds. Objective: To propose a protocol to evaluate efficacy in the treatment of experimental cutaneous wounds by means of pulsed therapeutic ultrasound of 3 MHz through 1.0W/cm2, 1.5W/cm2, 2.0W/cm2. This is an experimental and randomized study. Sixty male Wistar rats were used. After anesthetized, the animals underwent surgery to remove a fragment of the skin in the region of the animal's back with the use of dermatological punch in the 8. The animals were randomly randomized in 4 different groups being: control group; GA (1.0W/cm2); GB (1.5w / cm2) and GC (2.0w / cm2). The device was fixed to the other parameters: 3MHz, pulsed with 20% pulse with, 100Hz, for 2 minutes. The treatment was performed daily. The control group was manipulated with the device switched off. Each group was evaluated in 3 different time frames: 3rd, 7th and 15th postoperative day. Biometric, morphometric and stereological evaluations were performed. For the statistic, the PRISM 6.0 software was used with the TWO-WAY ANOVA test with BONFERRONI post-test considering p <0.05. In the evaluation of the variables for retraction of the wound area, retraction percentage as well as the daily retraction rate presented p> 0.05 when compared to the control group versus the experimental group. Regarding the thickness of the epithelium and keratin, there was no significant difference between the control group and the treated groups, however, groups irradiated with higher frequencies had worse results in the healing process. In this experimental model, the protocol for the use of therapeutic ultrasound interferes negatively in the process of wound healing when used in doses starting at 1.5W / cm2.No Brasil, as feridas constituem um sério problema de saúde pública, devido ao grande número de doentes com alterações na integridade da pele, embora sejam escassos os registros desses atendimentos. Na tentativa de minimizar o tempo de cicatrização tecidual, diversos estudos com animais vêm apresentando como fator coadjuvante a utilização do ultrassom terapêutico. Entretanto, não há padronização das doses para utilização dessa terapia em feridas cutâneas. Propor um protocolo para avaliar a eficácia no tratamento de feridas cutâneas experimentais por meio de ultrassom terapêutico pulsado de 3 MHz através de 1,0W/cm2, 1,5W/cm2, 2,0W/cm2. Trata-se de um estudo experimental e randomizado. Foram utilizados 60 ratos Wistar, machos, adultos. Após anestesiados, os animais foram submetidos a cirurgia para retirada de um fragmento da pele na região do dorso do animal com a utilização de punch dermatológico no 8. Os animais foram randomizados aleatoriamente em 4 grupos diferentes sendo eles: grupo controle; GA (1,0w/cm2); GB (1,5w/cm2) e GC (2,0w/cm2). O aparelho foi fixado nos demais parâmetros: 3MHz, pulsado com 20% largura de pulso, 100Hz, por 2 minutos. O tratamento foi realizado diariamente. O grupo controle foi manipulado com o aparelho desligado. Cada grupo foi avaliado em 3 diferentes espaços de tempo: 3o, 7o e 15o dia de pós-operatório. Foram feitas avaliações biométricas, morfométricas e estereológicas. Para a estatística foi utilizado o software PRISM 6.0 com o teste TWO-WAY ANOVA com pós-teste de BONFERRONI considerando p<0,05. Na avaliação das variáveis para retração da área da ferida, porcentagem de retração bem como a taxa diária de retração apresentaram p>0,05 quando comparado o grupo controle versus grupo experimental. Quanto à espessura do epitélio e queratina, não houve diferença significante entre o grupo controle e os grupos tratados, entretanto grupos irradiados com maiores frequências apresentaram piores resultados no processo de cicatrização. Neste modelo experimental, o protocolo de utilização do ultrassom terapêutico interfere negativamente no processo de cicatrização de feridas quando utilizados em doses a partir de 1,5W/cm2.Sustenere Publishing2023-06-19info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://sustenere.inf.br/index.php/sciresalutis/article/view/808010.6008/CBPC2236-9600.2023.001.0007Scire Salutis; Vol. 13 No. 1 (2023): Scire Salutis - Out, Nov, Dez 2022, Jan 2023; 59-73Scire Salutis; Vol. 13 Núm. 1 (2023): Scire Salutis - Out, Nov, Dez 2022, Jan 2023; 59-73Scire Salutis; v. 13 n. 1 (2023): Scire Salutis - Out, Nov, Dez 2022, Jan 2023; 59-732236-9600reponame:Scire Salutisinstname:Companhia Brasileira de Produção Científica (CBPC)instacron:ESSporenghttps://sustenere.inf.br/index.php/sciresalutis/article/view/8080/4407https://sustenere.inf.br/index.php/sciresalutis/article/view/8080/4406Copyright (c) 2023 Scire Salutishttp://creativecommons.org/licenses/by-nc-nd/4.0info:eu-repo/semantics/openAccessDantas, Lívia Carolina de Rodrigues, Célio Fernando de Sousa Omena, Clisivaldo Oliveira de Oliveira Neto, Olavo Barbosa de Lima, Vanessa Souza Barbosa, Fabiano Timbó 2023-12-29T22:29:59Zoai:ojs.pkp.sfu.ca:article/8080Revistahttps://sustenere.co/index.php/sciresalutisONGhttps://sustenere.co/index.php/sciresalutis/oai||carlos@arvore.org.br2236-96002236-9600opendoar:2023-12-29T22:29:59Scire Salutis - Companhia Brasileira de Produção Científica (CBPC)false |
dc.title.none.fl_str_mv |
Is the treatment of skin wounds with therapeutic ultrasound effective? Analysis and proposal of an experimental protocol O tratamento de feridas cutâneas com ultrassom terapêutico é eficaz? Análise e proposta de um protocolo experimental |
title |
Is the treatment of skin wounds with therapeutic ultrasound effective? Analysis and proposal of an experimental protocol |
spellingShingle |
Is the treatment of skin wounds with therapeutic ultrasound effective? Analysis and proposal of an experimental protocol Dantas, Lívia Carolina de Ultrassom terapêutico Feridas cutâneas Cicatrização Therapeutic ultrasound Cutaneous wounds Healing |
title_short |
Is the treatment of skin wounds with therapeutic ultrasound effective? Analysis and proposal of an experimental protocol |
title_full |
Is the treatment of skin wounds with therapeutic ultrasound effective? Analysis and proposal of an experimental protocol |
title_fullStr |
Is the treatment of skin wounds with therapeutic ultrasound effective? Analysis and proposal of an experimental protocol |
title_full_unstemmed |
Is the treatment of skin wounds with therapeutic ultrasound effective? Analysis and proposal of an experimental protocol |
title_sort |
Is the treatment of skin wounds with therapeutic ultrasound effective? Analysis and proposal of an experimental protocol |
author |
Dantas, Lívia Carolina de |
author_facet |
Dantas, Lívia Carolina de Rodrigues, Célio Fernando de Sousa Omena, Clisivaldo Oliveira de Oliveira Neto, Olavo Barbosa de Lima, Vanessa Souza Barbosa, Fabiano Timbó |
author_role |
author |
author2 |
Rodrigues, Célio Fernando de Sousa Omena, Clisivaldo Oliveira de Oliveira Neto, Olavo Barbosa de Lima, Vanessa Souza Barbosa, Fabiano Timbó |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Dantas, Lívia Carolina de Rodrigues, Célio Fernando de Sousa Omena, Clisivaldo Oliveira de Oliveira Neto, Olavo Barbosa de Lima, Vanessa Souza Barbosa, Fabiano Timbó |
dc.subject.por.fl_str_mv |
Ultrassom terapêutico Feridas cutâneas Cicatrização Therapeutic ultrasound Cutaneous wounds Healing |
topic |
Ultrassom terapêutico Feridas cutâneas Cicatrização Therapeutic ultrasound Cutaneous wounds Healing |
description |
In Brazil, wounds are a serious public health problem, due to the large number of patients with changes in skin integrity, although the records of these treatments are scarce. In an attempt to minimize tissue healing time, several animal studies have been presenting the use of therapeutic ultrasound as a coadjuvant factor. However, there is no standardization of doses for use of this therapy in cutaneous wounds. Objective: To propose a protocol to evaluate efficacy in the treatment of experimental cutaneous wounds by means of pulsed therapeutic ultrasound of 3 MHz through 1.0W/cm2, 1.5W/cm2, 2.0W/cm2. This is an experimental and randomized study. Sixty male Wistar rats were used. After anesthetized, the animals underwent surgery to remove a fragment of the skin in the region of the animal's back with the use of dermatological punch in the 8. The animals were randomly randomized in 4 different groups being: control group; GA (1.0W/cm2); GB (1.5w / cm2) and GC (2.0w / cm2). The device was fixed to the other parameters: 3MHz, pulsed with 20% pulse with, 100Hz, for 2 minutes. The treatment was performed daily. The control group was manipulated with the device switched off. Each group was evaluated in 3 different time frames: 3rd, 7th and 15th postoperative day. Biometric, morphometric and stereological evaluations were performed. For the statistic, the PRISM 6.0 software was used with the TWO-WAY ANOVA test with BONFERRONI post-test considering p <0.05. In the evaluation of the variables for retraction of the wound area, retraction percentage as well as the daily retraction rate presented p> 0.05 when compared to the control group versus the experimental group. Regarding the thickness of the epithelium and keratin, there was no significant difference between the control group and the treated groups, however, groups irradiated with higher frequencies had worse results in the healing process. In this experimental model, the protocol for the use of therapeutic ultrasound interferes negatively in the process of wound healing when used in doses starting at 1.5W / cm2. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-06-19 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://sustenere.inf.br/index.php/sciresalutis/article/view/8080 10.6008/CBPC2236-9600.2023.001.0007 |
url |
https://sustenere.inf.br/index.php/sciresalutis/article/view/8080 |
identifier_str_mv |
10.6008/CBPC2236-9600.2023.001.0007 |
dc.language.iso.fl_str_mv |
por eng |
language |
por eng |
dc.relation.none.fl_str_mv |
https://sustenere.inf.br/index.php/sciresalutis/article/view/8080/4407 https://sustenere.inf.br/index.php/sciresalutis/article/view/8080/4406 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2023 Scire Salutis http://creativecommons.org/licenses/by-nc-nd/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2023 Scire Salutis http://creativecommons.org/licenses/by-nc-nd/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Sustenere Publishing |
publisher.none.fl_str_mv |
Sustenere Publishing |
dc.source.none.fl_str_mv |
Scire Salutis; Vol. 13 No. 1 (2023): Scire Salutis - Out, Nov, Dez 2022, Jan 2023; 59-73 Scire Salutis; Vol. 13 Núm. 1 (2023): Scire Salutis - Out, Nov, Dez 2022, Jan 2023; 59-73 Scire Salutis; v. 13 n. 1 (2023): Scire Salutis - Out, Nov, Dez 2022, Jan 2023; 59-73 2236-9600 reponame:Scire Salutis instname:Companhia Brasileira de Produção Científica (CBPC) instacron:ESS |
instname_str |
Companhia Brasileira de Produção Científica (CBPC) |
instacron_str |
ESS |
institution |
ESS |
reponame_str |
Scire Salutis |
collection |
Scire Salutis |
repository.name.fl_str_mv |
Scire Salutis - Companhia Brasileira de Produção Científica (CBPC) |
repository.mail.fl_str_mv |
||carlos@arvore.org.br |
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