Is the treatment of skin wounds with therapeutic ultrasound effective? Analysis and proposal of an experimental protocol

• Autor(a) principal: Dantas, Lívia Carolina de
• Data de Publicação: 2023
• Outros Autores: Rodrigues, Célio Fernando de Sousa, Omena, Clisivaldo Oliveira de, Oliveira Neto, Olavo Barbosa de, Lima, Vanessa Souza, Barbosa, Fabiano Timbó
• Tipo de documento: Artigo
• Idioma: por; eng
• Título da fonte: Scire Salutis
• Texto Completo: https://sustenere.inf.br/index.php/sciresalutis/article/view/8080
• topic: Ultrassom terapêutico; Feridas cutâneas; Cicatrização; Therapeutic ultrasound; Cutaneous wounds; Healing

In Brazil, wounds are a serious public health problem, due to the large number of patients with changes in skin integrity, although the records of these treatments are scarce. In an attempt to minimize tissue healing time, several animal studies have been presenting the use of therapeutic ultrasound as a coadjuvant factor. However, there is no standardization of doses for use of this therapy in cutaneous wounds. Objective: To propose a protocol to evaluate efficacy in the treatment of experimental cutaneous wounds by means of pulsed therapeutic ultrasound of 3 MHz through 1.0W/cm2, 1.5W/cm2, 2.0W/cm2. This is an experimental and randomized study. Sixty male Wistar rats were used. After anesthetized, the animals underwent surgery to remove a fragment of the skin in the region of the animal's back with the use of dermatological punch in the 8. The animals were randomly randomized in 4 different groups being: control group; GA (1.0W/cm2); GB (1.5w / cm2) and GC (2.0w / cm2). The device was fixed to the other parameters: 3MHz, pulsed with 20% pulse with, 100Hz, for 2 minutes. The treatment was performed daily. The control group was manipulated with the device switched off. Each group was evaluated in 3 different time frames: 3rd, 7th and 15th postoperative day. Biometric, morphometric and stereological evaluations were performed. For the statistic, the PRISM 6.0 software was used with the TWO-WAY ANOVA test with BONFERRONI post-test considering p <0.05. In the evaluation of the variables for retraction of the wound area, retraction percentage as well as the daily retraction rate presented p> ​​0.05 when compared to the control group versus the experimental group. Regarding the thickness of the epithelium and keratin, there was no significant difference between the control group and the treated groups, however, groups irradiated with higher frequencies had worse results in the healing process. In this experimental model, the protocol for the use of therapeutic ultrasound interferes negatively in the process of wound healing when used in doses starting at 1.5W / cm2.