Assessment of serological response of chickens to Salmonella Gallinarum and Salmonella Pullorum by Elisa

Detalhes bibliográficos
Autor(a) principal: Oliveira,GH de<A NAME="nt01a"></A>
Data de Publicação: 2004
Outros Autores: Berchieri Júnior,Â, Montassier,HJ, Fernandes,AC
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Poultry Science (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2004000200007
Resumo: This study was done to assess an enzyme-linked immunosorbent assay (ELISA) to detect chicken serological response against Salmonella enterica serotypes Gallinarum and Pullorum. The assays have used soluble bacterial proteins of Salmonella Gallinarum strain 9 (AgSG) as detecting antigen and peroxidase and alkaline phosphatase conjugates. According to the results, the antigen, sera and conjugate concentrations were optimized. In addition, the assay using alkaline phosphatase and peroxidase conjugates was helpful to distinguish positive serological reaction to serotypes Gallinarum and Pullorum from Enteritidis.
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spelling Assessment of serological response of chickens to Salmonella Gallinarum and Salmonella Pullorum by ElisaChickensconjugateELISASalmonella spserologyThis study was done to assess an enzyme-linked immunosorbent assay (ELISA) to detect chicken serological response against Salmonella enterica serotypes Gallinarum and Pullorum. The assays have used soluble bacterial proteins of Salmonella Gallinarum strain 9 (AgSG) as detecting antigen and peroxidase and alkaline phosphatase conjugates. According to the results, the antigen, sera and conjugate concentrations were optimized. In addition, the assay using alkaline phosphatase and peroxidase conjugates was helpful to distinguish positive serological reaction to serotypes Gallinarum and Pullorum from Enteritidis.Fundacao de Apoio a Ciência e Tecnologia Avicolas2004-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2004000200007Brazilian Journal of Poultry Science v.6 n.2 2004reponame:Brazilian Journal of Poultry Science (Online)instname:Fundação APINCO de Ciência e Tecnologia Avícolas (FACTA)instacron:FACTA10.1590/S1516-635X2004000200007info:eu-repo/semantics/openAccessOliveira,GH de<A NAME="nt01a"></A>Berchieri Júnior,ÂMontassier,HJFernandes,ACeng2004-10-18T00:00:00Zoai:scielo:S1516-635X2004000200007Revistahttp://www.scielo.br/rbcahttps://old.scielo.br/oai/scielo-oai.php||rvfacta@terra.com.br1806-90611516-635Xopendoar:2004-10-18T00:00Brazilian Journal of Poultry Science (Online) - Fundação APINCO de Ciência e Tecnologia Avícolas (FACTA)false
dc.title.none.fl_str_mv Assessment of serological response of chickens to Salmonella Gallinarum and Salmonella Pullorum by Elisa
title Assessment of serological response of chickens to Salmonella Gallinarum and Salmonella Pullorum by Elisa
spellingShingle Assessment of serological response of chickens to Salmonella Gallinarum and Salmonella Pullorum by Elisa
Oliveira,GH de<A NAME="nt01a"></A>
Chickens
conjugate
ELISA
Salmonella sp
serology
title_short Assessment of serological response of chickens to Salmonella Gallinarum and Salmonella Pullorum by Elisa
title_full Assessment of serological response of chickens to Salmonella Gallinarum and Salmonella Pullorum by Elisa
title_fullStr Assessment of serological response of chickens to Salmonella Gallinarum and Salmonella Pullorum by Elisa
title_full_unstemmed Assessment of serological response of chickens to Salmonella Gallinarum and Salmonella Pullorum by Elisa
title_sort Assessment of serological response of chickens to Salmonella Gallinarum and Salmonella Pullorum by Elisa
author Oliveira,GH de<A NAME="nt01a"></A>
author_facet Oliveira,GH de<A NAME="nt01a"></A>
Berchieri Júnior,Â
Montassier,HJ
Fernandes,AC
author_role author
author2 Berchieri Júnior,Â
Montassier,HJ
Fernandes,AC
author2_role author
author
author
dc.contributor.author.fl_str_mv Oliveira,GH de<A NAME="nt01a"></A>
Berchieri Júnior,Â
Montassier,HJ
Fernandes,AC
dc.subject.por.fl_str_mv Chickens
conjugate
ELISA
Salmonella sp
serology
topic Chickens
conjugate
ELISA
Salmonella sp
serology
description This study was done to assess an enzyme-linked immunosorbent assay (ELISA) to detect chicken serological response against Salmonella enterica serotypes Gallinarum and Pullorum. The assays have used soluble bacterial proteins of Salmonella Gallinarum strain 9 (AgSG) as detecting antigen and peroxidase and alkaline phosphatase conjugates. According to the results, the antigen, sera and conjugate concentrations were optimized. In addition, the assay using alkaline phosphatase and peroxidase conjugates was helpful to distinguish positive serological reaction to serotypes Gallinarum and Pullorum from Enteritidis.
publishDate 2004
dc.date.none.fl_str_mv 2004-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2004000200007
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2004000200007
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-635X2004000200007
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Fundacao de Apoio a Ciência e Tecnologia Avicolas
publisher.none.fl_str_mv Fundacao de Apoio a Ciência e Tecnologia Avicolas
dc.source.none.fl_str_mv Brazilian Journal of Poultry Science v.6 n.2 2004
reponame:Brazilian Journal of Poultry Science (Online)
instname:Fundação APINCO de Ciência e Tecnologia Avícolas (FACTA)
instacron:FACTA
instname_str Fundação APINCO de Ciência e Tecnologia Avícolas (FACTA)
instacron_str FACTA
institution FACTA
reponame_str Brazilian Journal of Poultry Science (Online)
collection Brazilian Journal of Poultry Science (Online)
repository.name.fl_str_mv Brazilian Journal of Poultry Science (Online) - Fundação APINCO de Ciência e Tecnologia Avícolas (FACTA)
repository.mail.fl_str_mv ||rvfacta@terra.com.br
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