Assessment of serological response of chickens to Salmonella Gallinarum and Salmonella Pullorum by Elisa

Detalhes bibliográficos
Autor(a) principal: Oliveira, Gh De [UNESP]
Data de Publicação: 2004
Outros Autores: Berchieri Júnior, Â [UNESP], Montassier, Hj [UNESP], Fernandes, Ac [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
DOI: 10.1590/S1516-635X2004000200007
Texto Completo: http://dx.doi.org/10.1590/S1516-635X2004000200007
http://hdl.handle.net/11449/211719
Resumo: This study was done to assess an enzyme-linked immunosorbent assay (ELISA) to detect chicken serological response against Salmonella enterica serotypes Gallinarum and Pullorum. The assays have used soluble bacterial proteins of Salmonella Gallinarum strain 9 (AgSG) as detecting antigen and peroxidase and alkaline phosphatase conjugates. According to the results, the antigen, sera and conjugate concentrations were optimized. In addition, the assay using alkaline phosphatase and peroxidase conjugates was helpful to distinguish positive serological reaction to serotypes Gallinarum and Pullorum from Enteritidis.
id UNSP_398fc9d0040bf73f2846a55fd30836c6
oai_identifier_str oai:repositorio.unesp.br:11449/211719
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Assessment of serological response of chickens to Salmonella Gallinarum and Salmonella Pullorum by ElisaChickensconjugateELISASalmonella spserologyThis study was done to assess an enzyme-linked immunosorbent assay (ELISA) to detect chicken serological response against Salmonella enterica serotypes Gallinarum and Pullorum. The assays have used soluble bacterial proteins of Salmonella Gallinarum strain 9 (AgSG) as detecting antigen and peroxidase and alkaline phosphatase conjugates. According to the results, the antigen, sera and conjugate concentrations were optimized. In addition, the assay using alkaline phosphatase and peroxidase conjugates was helpful to distinguish positive serological reaction to serotypes Gallinarum and Pullorum from Enteritidis.Universidade Estadual Paulista, Faculdade de Ciências Agrárias e VeterináriasUniversidade Estadual Paulista, Faculdade de Ciências Agrárias e VeterináriasFundação APINCO de Ciência e Tecnologia AvícolasUniversidade Estadual Paulista (Unesp)Oliveira, Gh De [UNESP]Berchieri Júnior, Â [UNESP]Montassier, Hj [UNESP]Fernandes, Ac [UNESP]2021-07-14T10:28:35Z2021-07-14T10:28:35Z2004-06info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article111-115application/pdfhttp://dx.doi.org/10.1590/S1516-635X2004000200007Brazilian Journal of Poultry Science. Campinas, SP, Brazil: Fundação APINCO de Ciência e Tecnologia Avícolas, v. 6, n. 2, p. 111-115, 2004.1516-635X1806-9061http://hdl.handle.net/11449/21171910.1590/S1516-635X2004000200007S1516-635X2004000200007S1516-635X2004000200007.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal of Poultry Scienceinfo:eu-repo/semantics/openAccess2024-01-07T06:23:58Zoai:repositorio.unesp.br:11449/211719Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:20:21.887358Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Assessment of serological response of chickens to Salmonella Gallinarum and Salmonella Pullorum by Elisa
title Assessment of serological response of chickens to Salmonella Gallinarum and Salmonella Pullorum by Elisa
spellingShingle Assessment of serological response of chickens to Salmonella Gallinarum and Salmonella Pullorum by Elisa
Assessment of serological response of chickens to Salmonella Gallinarum and Salmonella Pullorum by Elisa
Oliveira, Gh De [UNESP]
Chickens
conjugate
ELISA
Salmonella sp
serology
Oliveira, Gh De [UNESP]
Chickens
conjugate
ELISA
Salmonella sp
serology
title_short Assessment of serological response of chickens to Salmonella Gallinarum and Salmonella Pullorum by Elisa
title_full Assessment of serological response of chickens to Salmonella Gallinarum and Salmonella Pullorum by Elisa
title_fullStr Assessment of serological response of chickens to Salmonella Gallinarum and Salmonella Pullorum by Elisa
Assessment of serological response of chickens to Salmonella Gallinarum and Salmonella Pullorum by Elisa
title_full_unstemmed Assessment of serological response of chickens to Salmonella Gallinarum and Salmonella Pullorum by Elisa
Assessment of serological response of chickens to Salmonella Gallinarum and Salmonella Pullorum by Elisa
title_sort Assessment of serological response of chickens to Salmonella Gallinarum and Salmonella Pullorum by Elisa
author Oliveira, Gh De [UNESP]
author_facet Oliveira, Gh De [UNESP]
Oliveira, Gh De [UNESP]
Berchieri Júnior, Â [UNESP]
Montassier, Hj [UNESP]
Fernandes, Ac [UNESP]
Berchieri Júnior, Â [UNESP]
Montassier, Hj [UNESP]
Fernandes, Ac [UNESP]
author_role author
author2 Berchieri Júnior, Â [UNESP]
Montassier, Hj [UNESP]
Fernandes, Ac [UNESP]
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Oliveira, Gh De [UNESP]
Berchieri Júnior, Â [UNESP]
Montassier, Hj [UNESP]
Fernandes, Ac [UNESP]
dc.subject.por.fl_str_mv Chickens
conjugate
ELISA
Salmonella sp
serology
topic Chickens
conjugate
ELISA
Salmonella sp
serology
description This study was done to assess an enzyme-linked immunosorbent assay (ELISA) to detect chicken serological response against Salmonella enterica serotypes Gallinarum and Pullorum. The assays have used soluble bacterial proteins of Salmonella Gallinarum strain 9 (AgSG) as detecting antigen and peroxidase and alkaline phosphatase conjugates. According to the results, the antigen, sera and conjugate concentrations were optimized. In addition, the assay using alkaline phosphatase and peroxidase conjugates was helpful to distinguish positive serological reaction to serotypes Gallinarum and Pullorum from Enteritidis.
publishDate 2004
dc.date.none.fl_str_mv 2004-06
2021-07-14T10:28:35Z
2021-07-14T10:28:35Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1516-635X2004000200007
Brazilian Journal of Poultry Science. Campinas, SP, Brazil: Fundação APINCO de Ciência e Tecnologia Avícolas, v. 6, n. 2, p. 111-115, 2004.
1516-635X
1806-9061
http://hdl.handle.net/11449/211719
10.1590/S1516-635X2004000200007
S1516-635X2004000200007
S1516-635X2004000200007.pdf
url http://dx.doi.org/10.1590/S1516-635X2004000200007
http://hdl.handle.net/11449/211719
identifier_str_mv Brazilian Journal of Poultry Science. Campinas, SP, Brazil: Fundação APINCO de Ciência e Tecnologia Avícolas, v. 6, n. 2, p. 111-115, 2004.
1516-635X
1806-9061
10.1590/S1516-635X2004000200007
S1516-635X2004000200007
S1516-635X2004000200007.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Brazilian Journal of Poultry Science
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 111-115
application/pdf
dc.publisher.none.fl_str_mv Fundação APINCO de Ciência e Tecnologia Avícolas
publisher.none.fl_str_mv Fundação APINCO de Ciência e Tecnologia Avícolas
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1822182553065357312
dc.identifier.doi.none.fl_str_mv 10.1590/S1516-635X2004000200007

Registros relacionados