Serum factors inhibitory for in vitro development of Plasmodium falciparum blood-stage parasites

Detalhes bibliográficos
Autor(a) principal: Perlaza,Blanca L.
Data de Publicação: 1994
Outros Autores: Plata,Cecilia de, Zapata,Constanza, Ramírez,Jaime A., Herrera,Socrates
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761994000600005
Resumo: Sera from 29 individuals residing in a malaria-endemic region of Colombia were evaluated by an inhibition assay for their capacity to retard the growth of Plasmodium falciparum in vitro. The inhibitory activity was found to be independent of antibody activity. Furthermore, the degree of inhibition of parasite development was variable, depending on the parasite isolate used for the assay and the season of malaria transmission. We selected sera with high inhibitory activity and carried out partial analytical characterization by anion exchange fast protein liquid chromatography (FPLC) to identify the chemical nature of the inhibitory factor(s). The results suggested that the in vitro inhibitory activity might result from the additive effect of different molecules. It appears that these molecules could be non-specifically induced by stimulation of the immune system, they seem to play a role in the immunity to malaria.
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spelling Serum factors inhibitory for in vitro development of Plasmodium falciparum blood-stage parasitesmalariaPlasmodium falciparumimmunitycrisis factorinhibitionSera from 29 individuals residing in a malaria-endemic region of Colombia were evaluated by an inhibition assay for their capacity to retard the growth of Plasmodium falciparum in vitro. The inhibitory activity was found to be independent of antibody activity. Furthermore, the degree of inhibition of parasite development was variable, depending on the parasite isolate used for the assay and the season of malaria transmission. We selected sera with high inhibitory activity and carried out partial analytical characterization by anion exchange fast protein liquid chromatography (FPLC) to identify the chemical nature of the inhibitory factor(s). The results suggested that the in vitro inhibitory activity might result from the additive effect of different molecules. It appears that these molecules could be non-specifically induced by stimulation of the immune system, they seem to play a role in the immunity to malaria.Instituto Oswaldo Cruz, Ministério da Saúde1994-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761994000600005Memórias do Instituto Oswaldo Cruz v.89 suppl.2 1994reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02761994000600005info:eu-repo/semantics/openAccessPerlaza,Blanca L.Plata,Cecilia deZapata,ConstanzaRamírez,Jaime A.Herrera,Socrateseng2020-04-25T17:47:19Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:06:16.37Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Serum factors inhibitory for in vitro development of Plasmodium falciparum blood-stage parasites
title Serum factors inhibitory for in vitro development of Plasmodium falciparum blood-stage parasites
spellingShingle Serum factors inhibitory for in vitro development of Plasmodium falciparum blood-stage parasites
Perlaza,Blanca L.
malaria
Plasmodium falciparum
immunity
crisis factor
inhibition
title_short Serum factors inhibitory for in vitro development of Plasmodium falciparum blood-stage parasites
title_full Serum factors inhibitory for in vitro development of Plasmodium falciparum blood-stage parasites
title_fullStr Serum factors inhibitory for in vitro development of Plasmodium falciparum blood-stage parasites
title_full_unstemmed Serum factors inhibitory for in vitro development of Plasmodium falciparum blood-stage parasites
title_sort Serum factors inhibitory for in vitro development of Plasmodium falciparum blood-stage parasites
author Perlaza,Blanca L.
author_facet Perlaza,Blanca L.
Plata,Cecilia de
Zapata,Constanza
Ramírez,Jaime A.
Herrera,Socrates
author_role author
author2 Plata,Cecilia de
Zapata,Constanza
Ramírez,Jaime A.
Herrera,Socrates
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Perlaza,Blanca L.
Plata,Cecilia de
Zapata,Constanza
Ramírez,Jaime A.
Herrera,Socrates
dc.subject.por.fl_str_mv malaria
Plasmodium falciparum
immunity
crisis factor
inhibition
topic malaria
Plasmodium falciparum
immunity
crisis factor
inhibition
dc.description.none.fl_txt_mv Sera from 29 individuals residing in a malaria-endemic region of Colombia were evaluated by an inhibition assay for their capacity to retard the growth of Plasmodium falciparum in vitro. The inhibitory activity was found to be independent of antibody activity. Furthermore, the degree of inhibition of parasite development was variable, depending on the parasite isolate used for the assay and the season of malaria transmission. We selected sera with high inhibitory activity and carried out partial analytical characterization by anion exchange fast protein liquid chromatography (FPLC) to identify the chemical nature of the inhibitory factor(s). The results suggested that the in vitro inhibitory activity might result from the additive effect of different molecules. It appears that these molecules could be non-specifically induced by stimulation of the immune system, they seem to play a role in the immunity to malaria.
description Sera from 29 individuals residing in a malaria-endemic region of Colombia were evaluated by an inhibition assay for their capacity to retard the growth of Plasmodium falciparum in vitro. The inhibitory activity was found to be independent of antibody activity. Furthermore, the degree of inhibition of parasite development was variable, depending on the parasite isolate used for the assay and the season of malaria transmission. We selected sera with high inhibitory activity and carried out partial analytical characterization by anion exchange fast protein liquid chromatography (FPLC) to identify the chemical nature of the inhibitory factor(s). The results suggested that the in vitro inhibitory activity might result from the additive effect of different molecules. It appears that these molecules could be non-specifically induced by stimulation of the immune system, they seem to play a role in the immunity to malaria.
publishDate 1994
dc.date.none.fl_str_mv 1994-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761994000600005
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761994000600005
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02761994000600005
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.89 suppl.2 1994
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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