Primary endemic Cryptococcosis gattii by molecular type VGII in the state of Pará, Brazil
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Memórias do Instituto Oswaldo Cruz |
Texto Completo: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762008000800012 |
Resumo: | In order to study the infectious agents causing human disseminated cryptococcosis in the state of Pará, North Brazil, 56 isolates of Cryptococcusspp. (54 isolated from cerebral spinal fluid and two from blood cultures) from 43 cases diagnosed between 2003-2007 were analysed. The species were determined through morphological and physiological tests and genotypes were determined by URA5-RFLP and PCR-fingerprinting (wild-type phage M13). The following species and genotypes were identified: Cryptococcus neoformans VNI (28/56, 50%), Cryptococcus gattii VGII (25/56, 44.64%) and C. gattii VGI (3/56, 5.26%). The genotype VNI occurred in 12 out of 14 HIV-positive adults, whereas the genotype VGII occurred in 11 out of 21 HIV-negative adults (p < 0.02, OR = 6.6 IC95% 0.98-56.0). All patients less than 12 years old were HIV negative and six cases were caused by the VGII genotype, one by the VGI and one by VNI. Therefore, endemic primary mycosis in HIV-negative individuals, including an unexpectedly high number of children, caused by the VGII genotype deserves further study and suggests the need for surveillance on cryptococcal infection in the state of Pará, Eastern Amazon. |
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Primary endemic Cryptococcosis gattii by molecular type VGII in the state of Pará, BrazilCryptococcus gattiiVGII genotypeendemic mycosischildrenstate of ParáIn order to study the infectious agents causing human disseminated cryptococcosis in the state of Pará, North Brazil, 56 isolates of Cryptococcusspp. (54 isolated from cerebral spinal fluid and two from blood cultures) from 43 cases diagnosed between 2003-2007 were analysed. The species were determined through morphological and physiological tests and genotypes were determined by URA5-RFLP and PCR-fingerprinting (wild-type phage M13). The following species and genotypes were identified: Cryptococcus neoformans VNI (28/56, 50%), Cryptococcus gattii VGII (25/56, 44.64%) and C. gattii VGI (3/56, 5.26%). The genotype VNI occurred in 12 out of 14 HIV-positive adults, whereas the genotype VGII occurred in 11 out of 21 HIV-negative adults (p < 0.02, OR = 6.6 IC95% 0.98-56.0). All patients less than 12 years old were HIV negative and six cases were caused by the VGII genotype, one by the VGI and one by VNI. Therefore, endemic primary mycosis in HIV-negative individuals, including an unexpectedly high number of children, caused by the VGII genotype deserves further study and suggests the need for surveillance on cryptococcal infection in the state of Pará, Eastern Amazon.Instituto Oswaldo Cruz, Ministério da Saúde2008-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762008000800012Memórias do Instituto Oswaldo Cruz v.103 n.8 2008reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762008000800012info:eu-repo/semantics/openAccessSantos,Wallace Raimundo A dosMeyer,WielandWanke,BodoCosta,Solange PS EvangelistaTrilles,LucianaNascimento,Jose Luiz MMedeiros,RitaMorales,Bernardina PernarrietaBezerra,Claudia de Carvalho FalciMacêdo,Regina Célia Lima deFerreira,Silvana OBarbosa,Gláucia GonçalvesPerez,Mauricio ANishikawa,Marília MartinsLazéra,Márcia dos Santoseng2020-04-25T17:50:22Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:15:53.04Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue |
dc.title.none.fl_str_mv |
Primary endemic Cryptococcosis gattii by molecular type VGII in the state of Pará, Brazil |
title |
Primary endemic Cryptococcosis gattii by molecular type VGII in the state of Pará, Brazil |
spellingShingle |
Primary endemic Cryptococcosis gattii by molecular type VGII in the state of Pará, Brazil Santos,Wallace Raimundo A dos Cryptococcus gattii VGII genotype endemic mycosis children state of Pará |
title_short |
Primary endemic Cryptococcosis gattii by molecular type VGII in the state of Pará, Brazil |
title_full |
Primary endemic Cryptococcosis gattii by molecular type VGII in the state of Pará, Brazil |
title_fullStr |
Primary endemic Cryptococcosis gattii by molecular type VGII in the state of Pará, Brazil |
title_full_unstemmed |
Primary endemic Cryptococcosis gattii by molecular type VGII in the state of Pará, Brazil |
title_sort |
Primary endemic Cryptococcosis gattii by molecular type VGII in the state of Pará, Brazil |
author |
Santos,Wallace Raimundo A dos |
author_facet |
Santos,Wallace Raimundo A dos Meyer,Wieland Wanke,Bodo Costa,Solange PS Evangelista Trilles,Luciana Nascimento,Jose Luiz M Medeiros,Rita Morales,Bernardina Pernarrieta Bezerra,Claudia de Carvalho Falci Macêdo,Regina Célia Lima de Ferreira,Silvana O Barbosa,Gláucia Gonçalves Perez,Mauricio A Nishikawa,Marília Martins Lazéra,Márcia dos Santos |
author_role |
author |
author2 |
Meyer,Wieland Wanke,Bodo Costa,Solange PS Evangelista Trilles,Luciana Nascimento,Jose Luiz M Medeiros,Rita Morales,Bernardina Pernarrieta Bezerra,Claudia de Carvalho Falci Macêdo,Regina Célia Lima de Ferreira,Silvana O Barbosa,Gláucia Gonçalves Perez,Mauricio A Nishikawa,Marília Martins Lazéra,Márcia dos Santos |
author2_role |
author author author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Santos,Wallace Raimundo A dos Meyer,Wieland Wanke,Bodo Costa,Solange PS Evangelista Trilles,Luciana Nascimento,Jose Luiz M Medeiros,Rita Morales,Bernardina Pernarrieta Bezerra,Claudia de Carvalho Falci Macêdo,Regina Célia Lima de Ferreira,Silvana O Barbosa,Gláucia Gonçalves Perez,Mauricio A Nishikawa,Marília Martins Lazéra,Márcia dos Santos |
dc.subject.por.fl_str_mv |
Cryptococcus gattii VGII genotype endemic mycosis children state of Pará |
topic |
Cryptococcus gattii VGII genotype endemic mycosis children state of Pará |
dc.description.none.fl_txt_mv |
In order to study the infectious agents causing human disseminated cryptococcosis in the state of Pará, North Brazil, 56 isolates of Cryptococcusspp. (54 isolated from cerebral spinal fluid and two from blood cultures) from 43 cases diagnosed between 2003-2007 were analysed. The species were determined through morphological and physiological tests and genotypes were determined by URA5-RFLP and PCR-fingerprinting (wild-type phage M13). The following species and genotypes were identified: Cryptococcus neoformans VNI (28/56, 50%), Cryptococcus gattii VGII (25/56, 44.64%) and C. gattii VGI (3/56, 5.26%). The genotype VNI occurred in 12 out of 14 HIV-positive adults, whereas the genotype VGII occurred in 11 out of 21 HIV-negative adults (p < 0.02, OR = 6.6 IC95% 0.98-56.0). All patients less than 12 years old were HIV negative and six cases were caused by the VGII genotype, one by the VGI and one by VNI. Therefore, endemic primary mycosis in HIV-negative individuals, including an unexpectedly high number of children, caused by the VGII genotype deserves further study and suggests the need for surveillance on cryptococcal infection in the state of Pará, Eastern Amazon. |
description |
In order to study the infectious agents causing human disseminated cryptococcosis in the state of Pará, North Brazil, 56 isolates of Cryptococcusspp. (54 isolated from cerebral spinal fluid and two from blood cultures) from 43 cases diagnosed between 2003-2007 were analysed. The species were determined through morphological and physiological tests and genotypes were determined by URA5-RFLP and PCR-fingerprinting (wild-type phage M13). The following species and genotypes were identified: Cryptococcus neoformans VNI (28/56, 50%), Cryptococcus gattii VGII (25/56, 44.64%) and C. gattii VGI (3/56, 5.26%). The genotype VNI occurred in 12 out of 14 HIV-positive adults, whereas the genotype VGII occurred in 11 out of 21 HIV-negative adults (p < 0.02, OR = 6.6 IC95% 0.98-56.0). All patients less than 12 years old were HIV negative and six cases were caused by the VGII genotype, one by the VGI and one by VNI. Therefore, endemic primary mycosis in HIV-negative individuals, including an unexpectedly high number of children, caused by the VGII genotype deserves further study and suggests the need for surveillance on cryptococcal infection in the state of Pará, Eastern Amazon. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762008000800012 |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762008000800012 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0074-02762008000800012 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
dc.source.none.fl_str_mv |
Memórias do Instituto Oswaldo Cruz v.103 n.8 2008 reponame:Memórias do Instituto Oswaldo Cruz instname:Fundação Oswaldo Cruz instacron:FIOCRUZ |
reponame_str |
Memórias do Instituto Oswaldo Cruz |
collection |
Memórias do Instituto Oswaldo Cruz |
instname_str |
Fundação Oswaldo Cruz |
instacron_str |
FIOCRUZ |
institution |
FIOCRUZ |
repository.name.fl_str_mv |
Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz |
repository.mail.fl_str_mv |
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