The in vitro cytopathology of a porcine and the simian (SA-11) strains of rotavirus

Detalhes bibliográficos
Autor(a) principal: Castilho,Juliana Galera
Data de Publicação: 2004
Outros Autores: Botelho,Maria Valéria Jacobucci, Lauretti,Flávio, Taniwaki,Noemi, Linhares,Rosa Elisa Carvalho, Nozawa,Carlos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762004000300013
Resumo: Rotaviruses have been implicated as the major causal agents of acute diarrhoea in mammals and fowls. Experimental rotavirus infection have been associated to a series of sub-cellular pathologic alterations leading to cell lysis which may represent key functions in the pathogenesis of the diarrhoeic disease. The current work describes the cytopathic changes in cultured MA-104 cells infected by a simian (SA-11) and a porcine (1154) rotavirus strains. Trypan blue exclusion staining showed increased cell permeability after infection by both strains, as demonstrated by cell viability. This effect was confirmed by the leakage of infected cells evaluated by chromium release. Nuclear fragmentation was observed by acridine orange and Wright staining but specific DNA cleavage was not detected. Ultrastructural changes, such as chromatin condensation, cytoplasm vacuolisation, and loss of intercellular contact were shown in infected cells for both strains. In situ terminal deoxynucleotidyl transferase (Tunel) assay did not show positive result. In conclusion, we demonstrated that both strains of rotavirus induced necrosis as the major degenerative effect.
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spelling The in vitro cytopathology of a porcine and the simian (SA-11) strains of rotavirusrotaviruscytopathologynecrosisapoptosiscell cultureRotaviruses have been implicated as the major causal agents of acute diarrhoea in mammals and fowls. Experimental rotavirus infection have been associated to a series of sub-cellular pathologic alterations leading to cell lysis which may represent key functions in the pathogenesis of the diarrhoeic disease. The current work describes the cytopathic changes in cultured MA-104 cells infected by a simian (SA-11) and a porcine (1154) rotavirus strains. Trypan blue exclusion staining showed increased cell permeability after infection by both strains, as demonstrated by cell viability. This effect was confirmed by the leakage of infected cells evaluated by chromium release. Nuclear fragmentation was observed by acridine orange and Wright staining but specific DNA cleavage was not detected. Ultrastructural changes, such as chromatin condensation, cytoplasm vacuolisation, and loss of intercellular contact were shown in infected cells for both strains. In situ terminal deoxynucleotidyl transferase (Tunel) assay did not show positive result. In conclusion, we demonstrated that both strains of rotavirus induced necrosis as the major degenerative effect.Instituto Oswaldo Cruz, Ministério da Saúde2004-05-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762004000300013Memórias do Instituto Oswaldo Cruz v.99 n.3 2004reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762004000300013info:eu-repo/semantics/openAccessCastilho,Juliana GaleraBotelho,Maria Valéria JacobucciLauretti,FlávioTaniwaki,NoemiLinhares,Rosa Elisa CarvalhoNozawa,Carloseng2020-04-25T17:49:15Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:12:35.948Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv The in vitro cytopathology of a porcine and the simian (SA-11) strains of rotavirus
title The in vitro cytopathology of a porcine and the simian (SA-11) strains of rotavirus
spellingShingle The in vitro cytopathology of a porcine and the simian (SA-11) strains of rotavirus
Castilho,Juliana Galera
rotavirus
cytopathology
necrosis
apoptosis
cell culture
title_short The in vitro cytopathology of a porcine and the simian (SA-11) strains of rotavirus
title_full The in vitro cytopathology of a porcine and the simian (SA-11) strains of rotavirus
title_fullStr The in vitro cytopathology of a porcine and the simian (SA-11) strains of rotavirus
title_full_unstemmed The in vitro cytopathology of a porcine and the simian (SA-11) strains of rotavirus
title_sort The in vitro cytopathology of a porcine and the simian (SA-11) strains of rotavirus
author Castilho,Juliana Galera
author_facet Castilho,Juliana Galera
Botelho,Maria Valéria Jacobucci
Lauretti,Flávio
Taniwaki,Noemi
Linhares,Rosa Elisa Carvalho
Nozawa,Carlos
author_role author
author2 Botelho,Maria Valéria Jacobucci
Lauretti,Flávio
Taniwaki,Noemi
Linhares,Rosa Elisa Carvalho
Nozawa,Carlos
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Castilho,Juliana Galera
Botelho,Maria Valéria Jacobucci
Lauretti,Flávio
Taniwaki,Noemi
Linhares,Rosa Elisa Carvalho
Nozawa,Carlos
dc.subject.por.fl_str_mv rotavirus
cytopathology
necrosis
apoptosis
cell culture
topic rotavirus
cytopathology
necrosis
apoptosis
cell culture
dc.description.none.fl_txt_mv Rotaviruses have been implicated as the major causal agents of acute diarrhoea in mammals and fowls. Experimental rotavirus infection have been associated to a series of sub-cellular pathologic alterations leading to cell lysis which may represent key functions in the pathogenesis of the diarrhoeic disease. The current work describes the cytopathic changes in cultured MA-104 cells infected by a simian (SA-11) and a porcine (1154) rotavirus strains. Trypan blue exclusion staining showed increased cell permeability after infection by both strains, as demonstrated by cell viability. This effect was confirmed by the leakage of infected cells evaluated by chromium release. Nuclear fragmentation was observed by acridine orange and Wright staining but specific DNA cleavage was not detected. Ultrastructural changes, such as chromatin condensation, cytoplasm vacuolisation, and loss of intercellular contact were shown in infected cells for both strains. In situ terminal deoxynucleotidyl transferase (Tunel) assay did not show positive result. In conclusion, we demonstrated that both strains of rotavirus induced necrosis as the major degenerative effect.
description Rotaviruses have been implicated as the major causal agents of acute diarrhoea in mammals and fowls. Experimental rotavirus infection have been associated to a series of sub-cellular pathologic alterations leading to cell lysis which may represent key functions in the pathogenesis of the diarrhoeic disease. The current work describes the cytopathic changes in cultured MA-104 cells infected by a simian (SA-11) and a porcine (1154) rotavirus strains. Trypan blue exclusion staining showed increased cell permeability after infection by both strains, as demonstrated by cell viability. This effect was confirmed by the leakage of infected cells evaluated by chromium release. Nuclear fragmentation was observed by acridine orange and Wright staining but specific DNA cleavage was not detected. Ultrastructural changes, such as chromatin condensation, cytoplasm vacuolisation, and loss of intercellular contact were shown in infected cells for both strains. In situ terminal deoxynucleotidyl transferase (Tunel) assay did not show positive result. In conclusion, we demonstrated that both strains of rotavirus induced necrosis as the major degenerative effect.
publishDate 2004
dc.date.none.fl_str_mv 2004-05-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762004000300013
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762004000300013
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02762004000300013
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.99 n.3 2004
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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