Study of some parameters affecting the in vitro cultivation of Plasmodium falciparum within saimiri sciureus red blood cells
Autor(a) principal: | |
---|---|
Data de Publicação: | 1986 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Memórias do Instituto Oswaldo Cruz |
Texto Completo: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761986000200005 |
Resumo: | The in vitro growth and multiplication of the erythrocytic stages of Plasmodium falciparum within Saimiri sciureus (squirrel monkey) red blood cells have been studied. Various parameters, such as the origin of the red blood cells and serum supplement, nature of the buffer, influence of the final pH of the medium, role of proteose peptone and glucose addition, were investigated. The selection of the best culture conditions led to the obtention of a reproducible in vitro growth of two parasite cycles in Saimiri erythrocytes, which is an useful achievement for in vitro studies. Our failure to establish a continuous culture line for longer than 19 days, could be explained by a dramatic increasing of osmotic fragility of the Saimiri red blood cells related to their small size. |
id |
FIOCRUZ-4_3a000f6eeec861718325130f895970fd |
---|---|
oai_identifier_str |
oai:scielo:S0074-02761986000200005 |
network_acronym_str |
FIOCRUZ-4 |
network_name_str |
Memórias do Instituto Oswaldo Cruz |
spelling |
Study of some parameters affecting the in vitro cultivation of Plasmodium falciparum within saimiri sciureus red blood cellsPlasmodium falciparumSaïmiri sciureusin vitro cultureSaïmiri erythrocytesThe in vitro growth and multiplication of the erythrocytic stages of Plasmodium falciparum within Saimiri sciureus (squirrel monkey) red blood cells have been studied. Various parameters, such as the origin of the red blood cells and serum supplement, nature of the buffer, influence of the final pH of the medium, role of proteose peptone and glucose addition, were investigated. The selection of the best culture conditions led to the obtention of a reproducible in vitro growth of two parasite cycles in Saimiri erythrocytes, which is an useful achievement for in vitro studies. Our failure to establish a continuous culture line for longer than 19 days, could be explained by a dramatic increasing of osmotic fragility of the Saimiri red blood cells related to their small size.Instituto Oswaldo Cruz, Ministério da Saúde1986-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761986000200005Memórias do Instituto Oswaldo Cruz v.81 n.2 1986reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02761986000200005info:eu-repo/semantics/openAccessFandeur,T.Dedet,J. P.eng2020-04-25T17:45:41Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:01:18.202Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue |
dc.title.none.fl_str_mv |
Study of some parameters affecting the in vitro cultivation of Plasmodium falciparum within saimiri sciureus red blood cells |
title |
Study of some parameters affecting the in vitro cultivation of Plasmodium falciparum within saimiri sciureus red blood cells |
spellingShingle |
Study of some parameters affecting the in vitro cultivation of Plasmodium falciparum within saimiri sciureus red blood cells Fandeur,T. Plasmodium falciparum Saïmiri sciureus in vitro culture Saïmiri erythrocytes |
title_short |
Study of some parameters affecting the in vitro cultivation of Plasmodium falciparum within saimiri sciureus red blood cells |
title_full |
Study of some parameters affecting the in vitro cultivation of Plasmodium falciparum within saimiri sciureus red blood cells |
title_fullStr |
Study of some parameters affecting the in vitro cultivation of Plasmodium falciparum within saimiri sciureus red blood cells |
title_full_unstemmed |
Study of some parameters affecting the in vitro cultivation of Plasmodium falciparum within saimiri sciureus red blood cells |
title_sort |
Study of some parameters affecting the in vitro cultivation of Plasmodium falciparum within saimiri sciureus red blood cells |
author |
Fandeur,T. |
author_facet |
Fandeur,T. Dedet,J. P. |
author_role |
author |
author2 |
Dedet,J. P. |
author2_role |
author |
dc.contributor.author.fl_str_mv |
Fandeur,T. Dedet,J. P. |
dc.subject.por.fl_str_mv |
Plasmodium falciparum Saïmiri sciureus in vitro culture Saïmiri erythrocytes |
topic |
Plasmodium falciparum Saïmiri sciureus in vitro culture Saïmiri erythrocytes |
dc.description.none.fl_txt_mv |
The in vitro growth and multiplication of the erythrocytic stages of Plasmodium falciparum within Saimiri sciureus (squirrel monkey) red blood cells have been studied. Various parameters, such as the origin of the red blood cells and serum supplement, nature of the buffer, influence of the final pH of the medium, role of proteose peptone and glucose addition, were investigated. The selection of the best culture conditions led to the obtention of a reproducible in vitro growth of two parasite cycles in Saimiri erythrocytes, which is an useful achievement for in vitro studies. Our failure to establish a continuous culture line for longer than 19 days, could be explained by a dramatic increasing of osmotic fragility of the Saimiri red blood cells related to their small size. |
description |
The in vitro growth and multiplication of the erythrocytic stages of Plasmodium falciparum within Saimiri sciureus (squirrel monkey) red blood cells have been studied. Various parameters, such as the origin of the red blood cells and serum supplement, nature of the buffer, influence of the final pH of the medium, role of proteose peptone and glucose addition, were investigated. The selection of the best culture conditions led to the obtention of a reproducible in vitro growth of two parasite cycles in Saimiri erythrocytes, which is an useful achievement for in vitro studies. Our failure to establish a continuous culture line for longer than 19 days, could be explained by a dramatic increasing of osmotic fragility of the Saimiri red blood cells related to their small size. |
publishDate |
1986 |
dc.date.none.fl_str_mv |
1986-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761986000200005 |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761986000200005 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0074-02761986000200005 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
dc.source.none.fl_str_mv |
Memórias do Instituto Oswaldo Cruz v.81 n.2 1986 reponame:Memórias do Instituto Oswaldo Cruz instname:Fundação Oswaldo Cruz instacron:FIOCRUZ |
reponame_str |
Memórias do Instituto Oswaldo Cruz |
collection |
Memórias do Instituto Oswaldo Cruz |
instname_str |
Fundação Oswaldo Cruz |
instacron_str |
FIOCRUZ |
institution |
FIOCRUZ |
repository.name.fl_str_mv |
Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz |
repository.mail.fl_str_mv |
|
_version_ |
1669937646508441600 |