Study of some parameters affecting the in vitro cultivation of Plasmodium falciparum within saimiri sciureus red blood cells

Detalhes bibliográficos
Autor(a) principal: Fandeur,T.
Data de Publicação: 1986
Outros Autores: Dedet,J. P.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761986000200005
Resumo: The in vitro growth and multiplication of the erythrocytic stages of Plasmodium falciparum within Saimiri sciureus (squirrel monkey) red blood cells have been studied. Various parameters, such as the origin of the red blood cells and serum supplement, nature of the buffer, influence of the final pH of the medium, role of proteose peptone and glucose addition, were investigated. The selection of the best culture conditions led to the obtention of a reproducible in vitro growth of two parasite cycles in Saimiri erythrocytes, which is an useful achievement for in vitro studies. Our failure to establish a continuous culture line for longer than 19 days, could be explained by a dramatic increasing of osmotic fragility of the Saimiri red blood cells related to their small size.
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spelling Study of some parameters affecting the in vitro cultivation of Plasmodium falciparum within saimiri sciureus red blood cellsPlasmodium falciparumSaïmiri sciureusin vitro cultureSaïmiri erythrocytesThe in vitro growth and multiplication of the erythrocytic stages of Plasmodium falciparum within Saimiri sciureus (squirrel monkey) red blood cells have been studied. Various parameters, such as the origin of the red blood cells and serum supplement, nature of the buffer, influence of the final pH of the medium, role of proteose peptone and glucose addition, were investigated. The selection of the best culture conditions led to the obtention of a reproducible in vitro growth of two parasite cycles in Saimiri erythrocytes, which is an useful achievement for in vitro studies. Our failure to establish a continuous culture line for longer than 19 days, could be explained by a dramatic increasing of osmotic fragility of the Saimiri red blood cells related to their small size.Instituto Oswaldo Cruz, Ministério da Saúde1986-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761986000200005Memórias do Instituto Oswaldo Cruz v.81 n.2 1986reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02761986000200005info:eu-repo/semantics/openAccessFandeur,T.Dedet,J. P.eng2020-04-25T17:45:41Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:01:18.202Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Study of some parameters affecting the in vitro cultivation of Plasmodium falciparum within saimiri sciureus red blood cells
title Study of some parameters affecting the in vitro cultivation of Plasmodium falciparum within saimiri sciureus red blood cells
spellingShingle Study of some parameters affecting the in vitro cultivation of Plasmodium falciparum within saimiri sciureus red blood cells
Fandeur,T.
Plasmodium falciparum
Saïmiri sciureus
in vitro culture
Saïmiri erythrocytes
title_short Study of some parameters affecting the in vitro cultivation of Plasmodium falciparum within saimiri sciureus red blood cells
title_full Study of some parameters affecting the in vitro cultivation of Plasmodium falciparum within saimiri sciureus red blood cells
title_fullStr Study of some parameters affecting the in vitro cultivation of Plasmodium falciparum within saimiri sciureus red blood cells
title_full_unstemmed Study of some parameters affecting the in vitro cultivation of Plasmodium falciparum within saimiri sciureus red blood cells
title_sort Study of some parameters affecting the in vitro cultivation of Plasmodium falciparum within saimiri sciureus red blood cells
author Fandeur,T.
author_facet Fandeur,T.
Dedet,J. P.
author_role author
author2 Dedet,J. P.
author2_role author
dc.contributor.author.fl_str_mv Fandeur,T.
Dedet,J. P.
dc.subject.por.fl_str_mv Plasmodium falciparum
Saïmiri sciureus
in vitro culture
Saïmiri erythrocytes
topic Plasmodium falciparum
Saïmiri sciureus
in vitro culture
Saïmiri erythrocytes
dc.description.none.fl_txt_mv The in vitro growth and multiplication of the erythrocytic stages of Plasmodium falciparum within Saimiri sciureus (squirrel monkey) red blood cells have been studied. Various parameters, such as the origin of the red blood cells and serum supplement, nature of the buffer, influence of the final pH of the medium, role of proteose peptone and glucose addition, were investigated. The selection of the best culture conditions led to the obtention of a reproducible in vitro growth of two parasite cycles in Saimiri erythrocytes, which is an useful achievement for in vitro studies. Our failure to establish a continuous culture line for longer than 19 days, could be explained by a dramatic increasing of osmotic fragility of the Saimiri red blood cells related to their small size.
description The in vitro growth and multiplication of the erythrocytic stages of Plasmodium falciparum within Saimiri sciureus (squirrel monkey) red blood cells have been studied. Various parameters, such as the origin of the red blood cells and serum supplement, nature of the buffer, influence of the final pH of the medium, role of proteose peptone and glucose addition, were investigated. The selection of the best culture conditions led to the obtention of a reproducible in vitro growth of two parasite cycles in Saimiri erythrocytes, which is an useful achievement for in vitro studies. Our failure to establish a continuous culture line for longer than 19 days, could be explained by a dramatic increasing of osmotic fragility of the Saimiri red blood cells related to their small size.
publishDate 1986
dc.date.none.fl_str_mv 1986-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761986000200005
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761986000200005
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02761986000200005
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.81 n.2 1986
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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